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Study of antagonistic effects of Lactobacillus strains as probiotics on multi drug resistant (MDR) bacteria isolated from urinary tract infections (UTIs).

Naderi A, Kasra-Kermanshahi R, Gharavi S, Imani Fooladi AA, Abdollahpour Alitappeh M, Saffarian P - Iran J Basic Med Sci (2014)

Bottom Line: However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics.In addition, L. casei was determined to be the most effective probiotic.Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene.

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology, Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.

ABSTRACT

Objective(s): Urinary tract infection (UTI) caused by bacteria is one of the most frequent infections in human population. Inappropriate use of antibiotics, often leads to appearance of drug resistance in bacteria. However, use of probiotic bacteria has been suggested as a partial replacement. This study was aimed to assess the antagonistic effects of Lactobacillus standard strains against bacteria isolated from UTI infections.

Materials and methods: Among 600 samples; those with ≥10,000 cfu/ml were selected as UTI positive samples. Enterococcus sp., Klebsiella pneumoniae, Enterobacter sp., and Escherichia coli were found the most prevalent UTI causative agents. All isolates were screened for multi drug resistance and subjected to the antimicrobial effects of three Lactobacillus strains by using microplate technique and the MICs amounts were determined. In order to verify the origin of antibiotic resistance of isolates, plasmid curing using ethidium bromide and acridine orange was carried out.

Results: No antagonistic activity in Lactobacilli suspension was detected against test on Enterococcus and Enterobacter strains and K. pneumoniae, which were resistant to most antibiotics. However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics. In addition, L. casei was determined to be the most effective probiotic. RESULTS from replica plating suggested one of the plasmids could be related to the gene responsible for ampicillin resistance.

Conclusion: Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene. Moreover, the plasmid profiles obtained from probiotic-treated isolates were identical to untreated isolates.

No MeSH data available.


Related in: MedlinePlus

Isolation of ciprofloxacin sensitive mutant strains created by treating the strain number 5 with Ethidium bromide and acridine orange using replica plating method. Plate A: plate without ciprofloxacin; plate B: plate containing ciprofloxacin
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Figure 7: Isolation of ciprofloxacin sensitive mutant strains created by treating the strain number 5 with Ethidium bromide and acridine orange using replica plating method. Plate A: plate without ciprofloxacin; plate B: plate containing ciprofloxacin

Mentions: Since the effect of treatment of E. coli strains with lactobacilli supernatants was not very considerable between the strains with patterns of resistance to eight or nine antibiotics, those strains which had larger inhibition halo were selected for molecular analyses (strains number 5 and 6). After treating the mentioned strains with ethidium bromide, the resulted mutant strains were isolated. Strain number 5 neither grew on the plates containing AM nor produced much smaller colonies than normal size (Figure 6), while strain number 6 grew normally. Both strains also grew on plates containing CP (Figure 7).


Study of antagonistic effects of Lactobacillus strains as probiotics on multi drug resistant (MDR) bacteria isolated from urinary tract infections (UTIs).

Naderi A, Kasra-Kermanshahi R, Gharavi S, Imani Fooladi AA, Abdollahpour Alitappeh M, Saffarian P - Iran J Basic Med Sci (2014)

Isolation of ciprofloxacin sensitive mutant strains created by treating the strain number 5 with Ethidium bromide and acridine orange using replica plating method. Plate A: plate without ciprofloxacin; plate B: plate containing ciprofloxacin
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016691&req=5

Figure 7: Isolation of ciprofloxacin sensitive mutant strains created by treating the strain number 5 with Ethidium bromide and acridine orange using replica plating method. Plate A: plate without ciprofloxacin; plate B: plate containing ciprofloxacin
Mentions: Since the effect of treatment of E. coli strains with lactobacilli supernatants was not very considerable between the strains with patterns of resistance to eight or nine antibiotics, those strains which had larger inhibition halo were selected for molecular analyses (strains number 5 and 6). After treating the mentioned strains with ethidium bromide, the resulted mutant strains were isolated. Strain number 5 neither grew on the plates containing AM nor produced much smaller colonies than normal size (Figure 6), while strain number 6 grew normally. Both strains also grew on plates containing CP (Figure 7).

Bottom Line: However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics.In addition, L. casei was determined to be the most effective probiotic.Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene.

View Article: PubMed Central - PubMed

Affiliation: Division of Microbiology, Department of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran.

ABSTRACT

Objective(s): Urinary tract infection (UTI) caused by bacteria is one of the most frequent infections in human population. Inappropriate use of antibiotics, often leads to appearance of drug resistance in bacteria. However, use of probiotic bacteria has been suggested as a partial replacement. This study was aimed to assess the antagonistic effects of Lactobacillus standard strains against bacteria isolated from UTI infections.

Materials and methods: Among 600 samples; those with ≥10,000 cfu/ml were selected as UTI positive samples. Enterococcus sp., Klebsiella pneumoniae, Enterobacter sp., and Escherichia coli were found the most prevalent UTI causative agents. All isolates were screened for multi drug resistance and subjected to the antimicrobial effects of three Lactobacillus strains by using microplate technique and the MICs amounts were determined. In order to verify the origin of antibiotic resistance of isolates, plasmid curing using ethidium bromide and acridine orange was carried out.

Results: No antagonistic activity in Lactobacilli suspension was detected against test on Enterococcus and Enterobacter strains and K. pneumoniae, which were resistant to most antibiotics. However, an inhibitory effect was observed for E. coli which were resistant to 8-9 antibiotics. In addition, L. casei was determined to be the most effective probiotic. RESULTS from replica plating suggested one of the plasmids could be related to the gene responsible for ampicillin resistance.

Conclusion: Treatment of E. coli with probiotic suspension was not effective on inhibition of the plasmid carrying hypothetical ampicillin resistant gene. Moreover, the plasmid profiles obtained from probiotic-treated isolates were identical to untreated isolates.

No MeSH data available.


Related in: MedlinePlus