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Immunohistochemical study of type III collagen expression during pre and post-natal rat skin morphogenesis.

Mohammadzadeh E, Nikravesh MR, Jalali M, Fazel A, Ebrahimi V, Ebrahimzadeh-Bideskan AR - Iran J Basic Med Sci (2014)

Bottom Line: Kruskal-Wallis non-parametric statistical test and SPSS software version 11.5 were used to compare differences between samples.This immunoreactivity pattern was increased afterward on E16, not changed on E18 and decreased in dermal reticularis on E20.Our results showed that type III collagen is expressed and timely regulated during pre and post natal rat skin morphogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objective(s): Skin extracellular matrix, which contains type I and type III collagens, is involved in skin development. The aim of this study was to investigate type III collagen distribution pattern as well as its changes during pre and post-natal skin morphogenesis in rats.

Materials and methods: Ventral skins of Wistar rat embryos at different stages from 10 to 20 gestational day (E10-E20) and also one month and one year post natal rat pups were fixed in normalin, embedded in paraffin and 5 µm thick sections were incubated with Anti type III collagen antibody. In order to detect staining intensity, the reactions were observed and graded by three examiners separately. Kruskal-Wallis non-parametric statistical test and SPSS software version 11.5 were used to compare differences between samples.

Results: Immunoreactivity of type III collagen was distributed weakly in the mesenchymal connective tissue on day 10 (E10). The observed reaction was increased onE12 and E14. This reaction was clear in basement membrane, relatively intensive in dermal papillae and moderate in dermal reticularis on E14. This immunoreactivity pattern was increased afterward on E16, not changed on E18 and decreased in dermal reticularis on E20. The density of collagen type III in dermal papillae and dermal reticularis in skin of one year old rats were decreased comparing to one month old rats.

Conclusion: Our results showed that type III collagen is expressed and timely regulated during pre and post natal rat skin morphogenesis.

No MeSH data available.


Photomicrographs that show the immunolocalization of type III collagen of rat developing skin A, B, C, D, E and F are representative of days 10, 12, 14, 16, 18 and 20 of gestation, respectively. Immunoreactivity is shown with thin arrow in corium in A, B , and C and dermal papillae in D, E, and F. Thick arrow points to immunoreactivity in dermal reticularis, collagen(C)
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Figure 1: Photomicrographs that show the immunolocalization of type III collagen of rat developing skin A, B, C, D, E and F are representative of days 10, 12, 14, 16, 18 and 20 of gestation, respectively. Immunoreactivity is shown with thin arrow in corium in A, B , and C and dermal papillae in D, E, and F. Thick arrow points to immunoreactivity in dermal reticularis, collagen(C)

Mentions: Immunohistochemical staining showed changes of type III collagen distribution in the developing skin on various gestational days from E10 to E20. In fetuses on E10, immunoreactivity specific for type III collagen was weakly and sparsely distributed in the mesenchymal connective tissue (1.125±0.6292) and it was relatively distinct on a fairly wide region of mesenchymal connective tissue just beneath the epithelium of the developing skin, where will be formed dermal papillae (Figure 1A). The observed reaction for type III collagen, which had been seen in the mesenchymal tissue behind the epithelium, was slightly stronger in fetuses on E12 comparing to E10 (1.25±0.5000) but it was not significant. At this embryonic stage, the embryos were covered with an epithelium composed of one layer of cuboidal cells (ectoderm) which reacted with hematoxilin and could be clearly distinguished but showed no immunoreactivity (Figure 1B).


Immunohistochemical study of type III collagen expression during pre and post-natal rat skin morphogenesis.

Mohammadzadeh E, Nikravesh MR, Jalali M, Fazel A, Ebrahimi V, Ebrahimzadeh-Bideskan AR - Iran J Basic Med Sci (2014)

Photomicrographs that show the immunolocalization of type III collagen of rat developing skin A, B, C, D, E and F are representative of days 10, 12, 14, 16, 18 and 20 of gestation, respectively. Immunoreactivity is shown with thin arrow in corium in A, B , and C and dermal papillae in D, E, and F. Thick arrow points to immunoreactivity in dermal reticularis, collagen(C)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016690&req=5

Figure 1: Photomicrographs that show the immunolocalization of type III collagen of rat developing skin A, B, C, D, E and F are representative of days 10, 12, 14, 16, 18 and 20 of gestation, respectively. Immunoreactivity is shown with thin arrow in corium in A, B , and C and dermal papillae in D, E, and F. Thick arrow points to immunoreactivity in dermal reticularis, collagen(C)
Mentions: Immunohistochemical staining showed changes of type III collagen distribution in the developing skin on various gestational days from E10 to E20. In fetuses on E10, immunoreactivity specific for type III collagen was weakly and sparsely distributed in the mesenchymal connective tissue (1.125±0.6292) and it was relatively distinct on a fairly wide region of mesenchymal connective tissue just beneath the epithelium of the developing skin, where will be formed dermal papillae (Figure 1A). The observed reaction for type III collagen, which had been seen in the mesenchymal tissue behind the epithelium, was slightly stronger in fetuses on E12 comparing to E10 (1.25±0.5000) but it was not significant. At this embryonic stage, the embryos were covered with an epithelium composed of one layer of cuboidal cells (ectoderm) which reacted with hematoxilin and could be clearly distinguished but showed no immunoreactivity (Figure 1B).

Bottom Line: Kruskal-Wallis non-parametric statistical test and SPSS software version 11.5 were used to compare differences between samples.This immunoreactivity pattern was increased afterward on E16, not changed on E18 and decreased in dermal reticularis on E20.Our results showed that type III collagen is expressed and timely regulated during pre and post natal rat skin morphogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objective(s): Skin extracellular matrix, which contains type I and type III collagens, is involved in skin development. The aim of this study was to investigate type III collagen distribution pattern as well as its changes during pre and post-natal skin morphogenesis in rats.

Materials and methods: Ventral skins of Wistar rat embryos at different stages from 10 to 20 gestational day (E10-E20) and also one month and one year post natal rat pups were fixed in normalin, embedded in paraffin and 5 µm thick sections were incubated with Anti type III collagen antibody. In order to detect staining intensity, the reactions were observed and graded by three examiners separately. Kruskal-Wallis non-parametric statistical test and SPSS software version 11.5 were used to compare differences between samples.

Results: Immunoreactivity of type III collagen was distributed weakly in the mesenchymal connective tissue on day 10 (E10). The observed reaction was increased onE12 and E14. This reaction was clear in basement membrane, relatively intensive in dermal papillae and moderate in dermal reticularis on E14. This immunoreactivity pattern was increased afterward on E16, not changed on E18 and decreased in dermal reticularis on E20. The density of collagen type III in dermal papillae and dermal reticularis in skin of one year old rats were decreased comparing to one month old rats.

Conclusion: Our results showed that type III collagen is expressed and timely regulated during pre and post natal rat skin morphogenesis.

No MeSH data available.