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Stereological analysis of cornu ammonis in prenatally stressed rats: a heuristic neurodevelopmental model of schizophrenia.

Hosseini-Sharifabad M, Sabahi A - Iran J Basic Med Sci (2014)

Bottom Line: Controls remained in the animal room and were exposed only to normal animal room conditions.Cavalieri's principle, physical disector and nucleator were applied for stereological analyses.However, there were no markedly differences in the numerical density, total number of CA3 pyramidal neurons and stereological parameters in CA1 of prenatally stressed and control animals.

View Article: PubMed Central - PubMed

ABSTRACT

Objective(s): The hippocampus has been implicated in pathophysiology of schizophrenia. Prenatal stress is a contributing risk factor for a wide variety of neuropsychiatric diseases including schizophrenia. This study examined long-term effects of prenatal restraint stress on the stereological parameters in the Cornu Ammonis (CA) of adult male rats as an animal model of schizophrenia.

Materials and methods: Wistar pregnant dams in experimental group were stressed in a cylindrical Plexiglas restrainer daily for 1 hr during last week of gestation. Controls remained in the animal room and were exposed only to normal animal room conditions. At 2 months of age, the volume of the pyramidal cell layer of the CA, the numerical density and the somal volume of the respective neurons were assessed in the male offspring generated from stressed and control pregnancies. Cavalieri's principle, physical disector and nucleator were applied for stereological analyses.

Results: This study showed that prenatal stress significantly decreased the volume of CA3 pyramidal cell layer and the individual somal volume of CA3 pyramidal neurons. However, there were no markedly differences in the numerical density, total number of CA3 pyramidal neurons and stereological parameters in CA1 of prenatally stressed and control animals.

Conclusion: These data indicate that prenatal stress exposure induced neuronal changes in the CA3 subfield of hippocampus which are similar to what is observed in schizophrenia.

No MeSH data available.


Related in: MedlinePlus

Low-power photographs of the hippocampi of control (a) and prenatally stressed rats (b). Photograph (a) also illustrates neuron containing layers of the hippocamal subdivisions (DG; Dentate gyrus, CA; Cornu Ammonis). Higher magnification (×1800) of CA1 and CA3 pyramidal cells shown in the left and right upper boxes respectively. Scale bar =600 µm and applies to both frames
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Figure 1: Low-power photographs of the hippocampi of control (a) and prenatally stressed rats (b). Photograph (a) also illustrates neuron containing layers of the hippocamal subdivisions (DG; Dentate gyrus, CA; Cornu Ammonis). Higher magnification (×1800) of CA1 and CA3 pyramidal cells shown in the left and right upper boxes respectively. Scale bar =600 µm and applies to both frames

Mentions: Discrimination between the different subdivisions of the hippocampal formation was based on cell morphology (27). Cornu Ammonis (CA) was subdivided into CA1, CA2, and CA3 regions (Figure 1). The CA2 region was considered as belonging to the CA3 region because the boundaries between these two fields of the hippocampus are not discrete in conventionally stained sections.


Stereological analysis of cornu ammonis in prenatally stressed rats: a heuristic neurodevelopmental model of schizophrenia.

Hosseini-Sharifabad M, Sabahi A - Iran J Basic Med Sci (2014)

Low-power photographs of the hippocampi of control (a) and prenatally stressed rats (b). Photograph (a) also illustrates neuron containing layers of the hippocamal subdivisions (DG; Dentate gyrus, CA; Cornu Ammonis). Higher magnification (×1800) of CA1 and CA3 pyramidal cells shown in the left and right upper boxes respectively. Scale bar =600 µm and applies to both frames
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016689&req=5

Figure 1: Low-power photographs of the hippocampi of control (a) and prenatally stressed rats (b). Photograph (a) also illustrates neuron containing layers of the hippocamal subdivisions (DG; Dentate gyrus, CA; Cornu Ammonis). Higher magnification (×1800) of CA1 and CA3 pyramidal cells shown in the left and right upper boxes respectively. Scale bar =600 µm and applies to both frames
Mentions: Discrimination between the different subdivisions of the hippocampal formation was based on cell morphology (27). Cornu Ammonis (CA) was subdivided into CA1, CA2, and CA3 regions (Figure 1). The CA2 region was considered as belonging to the CA3 region because the boundaries between these two fields of the hippocampus are not discrete in conventionally stained sections.

Bottom Line: Controls remained in the animal room and were exposed only to normal animal room conditions.Cavalieri's principle, physical disector and nucleator were applied for stereological analyses.However, there were no markedly differences in the numerical density, total number of CA3 pyramidal neurons and stereological parameters in CA1 of prenatally stressed and control animals.

View Article: PubMed Central - PubMed

ABSTRACT

Objective(s): The hippocampus has been implicated in pathophysiology of schizophrenia. Prenatal stress is a contributing risk factor for a wide variety of neuropsychiatric diseases including schizophrenia. This study examined long-term effects of prenatal restraint stress on the stereological parameters in the Cornu Ammonis (CA) of adult male rats as an animal model of schizophrenia.

Materials and methods: Wistar pregnant dams in experimental group were stressed in a cylindrical Plexiglas restrainer daily for 1 hr during last week of gestation. Controls remained in the animal room and were exposed only to normal animal room conditions. At 2 months of age, the volume of the pyramidal cell layer of the CA, the numerical density and the somal volume of the respective neurons were assessed in the male offspring generated from stressed and control pregnancies. Cavalieri's principle, physical disector and nucleator were applied for stereological analyses.

Results: This study showed that prenatal stress significantly decreased the volume of CA3 pyramidal cell layer and the individual somal volume of CA3 pyramidal neurons. However, there were no markedly differences in the numerical density, total number of CA3 pyramidal neurons and stereological parameters in CA1 of prenatally stressed and control animals.

Conclusion: These data indicate that prenatal stress exposure induced neuronal changes in the CA3 subfield of hippocampus which are similar to what is observed in schizophrenia.

No MeSH data available.


Related in: MedlinePlus