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In silico analysis of chimeric TF, Omp31 and BP26 fragments of Brucella melitensis for development of a multi subunit vaccine candidate.

Ghasemi A, Ranjbar R, Amani J - Iran J Basic Med Sci (2014)

Bottom Line: Validation results showed that 91.1% of residues lie in favored or additional allowed region of Ramachandran plot.The epitopes in the chimeric protein are likely to induce both the B-cell and T-cell mediated immune responses.Conclusion : The chimeric protein may be used as multi subunit for development of Brucella vaccine candidates.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objective(s): Brucellosis, especially caused by Brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. The commonly used live attenuated vaccine in ovine brucellosis prophylaxis is B. melitensis Rev1. But due to different problems caused by the administration of this vaccine, a protective subunit vaccine against B. melitensis is strongly demanded. Brucella BP26, Omp31 and TF proteins have shown a considerable potential as protective antigens for brucellosis. Chimeric proteins carrying epitopes or adjuvant sequences increase the possibility of eliciting a broad cellular or humoral immune response. In silico tools are highly suited to study, design and evaluate vaccine strategies.

Materials and methods: In this study, a synthetic chimeric gene, encoding TF, BP26 (93-111) and Omp31(48-74) was designed. In order to predict the 3D structure of protein, modeling was carried out.

Results: Validation results showed that 91.1% of residues lie in favored or additional allowed region of Ramachandran plot. The epitopes in the chimeric protein are likely to induce both the B-cell and T-cell mediated immune responses. Conclusion : The chimeric protein may be used as multi subunit for development of Brucella vaccine candidates.

No MeSH data available.


Related in: MedlinePlus

Analysis of mRNA stability and start codon in the structure
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Figure 2: Analysis of mRNA stability and start codon in the structure

Mentions: In order to determine the potential folding of the chimeric gene, genetic algorithm-based RNA secondary structure prediction was combined with comparative sequence analysis. The 5' terminus of the gene was folded typically as in all bacterial gene structures. Prediction of the minimum free energy for secondary structures formed by RNA molecules was also carried out. All 15 structural elements obtained in this analysis revealed folding of the RNA construct. ΔG of the best predicted structure was −217.92 kcal/mol and the first nucleotides at 5′ did not have a long stable hairpin or pseudoknot (Figure 2) (42).


In silico analysis of chimeric TF, Omp31 and BP26 fragments of Brucella melitensis for development of a multi subunit vaccine candidate.

Ghasemi A, Ranjbar R, Amani J - Iran J Basic Med Sci (2014)

Analysis of mRNA stability and start codon in the structure
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016687&req=5

Figure 2: Analysis of mRNA stability and start codon in the structure
Mentions: In order to determine the potential folding of the chimeric gene, genetic algorithm-based RNA secondary structure prediction was combined with comparative sequence analysis. The 5' terminus of the gene was folded typically as in all bacterial gene structures. Prediction of the minimum free energy for secondary structures formed by RNA molecules was also carried out. All 15 structural elements obtained in this analysis revealed folding of the RNA construct. ΔG of the best predicted structure was −217.92 kcal/mol and the first nucleotides at 5′ did not have a long stable hairpin or pseudoknot (Figure 2) (42).

Bottom Line: Validation results showed that 91.1% of residues lie in favored or additional allowed region of Ramachandran plot.The epitopes in the chimeric protein are likely to induce both the B-cell and T-cell mediated immune responses.Conclusion : The chimeric protein may be used as multi subunit for development of Brucella vaccine candidates.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objective(s): Brucellosis, especially caused by Brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. The commonly used live attenuated vaccine in ovine brucellosis prophylaxis is B. melitensis Rev1. But due to different problems caused by the administration of this vaccine, a protective subunit vaccine against B. melitensis is strongly demanded. Brucella BP26, Omp31 and TF proteins have shown a considerable potential as protective antigens for brucellosis. Chimeric proteins carrying epitopes or adjuvant sequences increase the possibility of eliciting a broad cellular or humoral immune response. In silico tools are highly suited to study, design and evaluate vaccine strategies.

Materials and methods: In this study, a synthetic chimeric gene, encoding TF, BP26 (93-111) and Omp31(48-74) was designed. In order to predict the 3D structure of protein, modeling was carried out.

Results: Validation results showed that 91.1% of residues lie in favored or additional allowed region of Ramachandran plot. The epitopes in the chimeric protein are likely to induce both the B-cell and T-cell mediated immune responses. Conclusion : The chimeric protein may be used as multi subunit for development of Brucella vaccine candidates.

No MeSH data available.


Related in: MedlinePlus