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ABCG5 gene responses to treadmill running with or without administration of Pistachio atlantica in female rats.

Ghanbari-Niaki A, Zare-Kookandeh N, Zare-Kookandeh A - Iran J Basic Med Sci (2014)

Bottom Line: The current findings indicate that the Baneh-treated tissues had significantly lower levels of ABCG5 gene expression in the liver, small intestine, and kidneys (P< 0.001, P< 0.003, P< 0.001, respectively), when compared with saline-treated tissues.A lower level of HDL-c but not triglyceride (TG) and total cholesterol (TC) levels were found in Baneh-treated animals at rest.Exercise training increases ABCG5 relative gene expression in the liver, small intestine and kidney tissues; therefore exercise training may adjust the reduction of ABCG5 relative gene expression in Baneh-training group.

View Article: PubMed Central - PubMed

Affiliation: Exercise Biochemistry Division, Faculty of Physical Education and Sport Science, University of Mazandaran, Baboulsar, Mazandaran, Iran.

ABSTRACT

Objective(s): ABC transporters comprise a large family of transmembrane proteins that use the energy provided by ATP hydrolysis to translocate a variety of substrates across biological membranes. All members of the human ABCG subfamily, except for ABCG2, are cholesterol-transporter. The aim of this study was to determine the liver, the small intestine and kidney ABCG5 relative gene expression in response to treadmill-running training in female rats.

Materials and methods: Twenty Wistar rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned to saline-control (SC), saline-training (ST), and Baneh-control (BC), and Baneh-training (BT) groups. Training groups did the exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day for eight weeks (5 days/week). Rats were fed orally, with Baneh extraction and saline for six weeks. The two-way ANOVA was employed for statistical analysis. ABCG5 relative gene expression was detected by Real-time PCR method.

Results: The current findings indicate that the Baneh-treated tissues had significantly lower levels of ABCG5 gene expression in the liver, small intestine, and kidneys (P< 0.001, P< 0.003, P< 0.001, respectively), when compared with saline-treated tissues. However, a higher level of gene expression was observed in exercise groups. A lower level of HDL-c but not triglyceride (TG) and total cholesterol (TC) levels were found in Baneh-treated animals at rest.

Conclusion: Exercise training increases ABCG5 relative gene expression in the liver, small intestine and kidney tissues; therefore exercise training may adjust the reduction of ABCG5 relative gene expression in Baneh-training group.

No MeSH data available.


Related in: MedlinePlus

Real-time PCR kidney ABCG5 mRNA relative expression in saline- control (SC), saline-training (ST), Baneh-control (BC), and Baneh-training (BT) wild-type female rats. Wild-type female rats data expressed as mean ± SEM. Each column is assigned to one group and 5 rats per each group) **,ST vs BT, (P < 0.003) +, BT vs BC , (P < 0.015)
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Figure 3: Real-time PCR kidney ABCG5 mRNA relative expression in saline- control (SC), saline-training (ST), Baneh-control (BC), and Baneh-training (BT) wild-type female rats. Wild-type female rats data expressed as mean ± SEM. Each column is assigned to one group and 5 rats per each group) **,ST vs BT, (P < 0.003) +, BT vs BC , (P < 0.015)

Mentions: ABCG5 relative gene expression in the liver, small intestine and kidney were determined in female rats. Data analysis revealed a significant difference in liver ABCG5 mRNA relative abundance between groups (F=14.50, P<0.001) (Figure 1). Using a suitable post hoc test, it was shown that the liver relative expression of ABCG5 was higher in ST group (2.2813±0.2285) when compared with other groups at the end of the experiment (Figure 1). Also, a significant difference was found in the small intestine relative mRNA expression of ABCG5 at the end of treadmill running program (F=8.158, P<0.003). In this regard, the ABCG5 mRNA relative abundance was lower in Baneh treated animals (1.0219±0.21305) when compared with saline-treated groups (1.4928±0.14083) (Figure 2). Considering the kidneys, the same results as the liver and intestine have been observed (F=17.61, P<0.001) (Figure 3). The levels of plasma HDL-c were significantly (P<0.05) lower in Baneh-treated animals when compare with their counterparts (Figure 4). There were no significant changes in plasma TG and TC concentrations, (Figure 5 and 6). Collectively, it should be noted that training groups, particularly saline-trained tissues had higher levels of ABCG5 gene expression than other groups.


ABCG5 gene responses to treadmill running with or without administration of Pistachio atlantica in female rats.

Ghanbari-Niaki A, Zare-Kookandeh N, Zare-Kookandeh A - Iran J Basic Med Sci (2014)

Real-time PCR kidney ABCG5 mRNA relative expression in saline- control (SC), saline-training (ST), Baneh-control (BC), and Baneh-training (BT) wild-type female rats. Wild-type female rats data expressed as mean ± SEM. Each column is assigned to one group and 5 rats per each group) **,ST vs BT, (P < 0.003) +, BT vs BC , (P < 0.015)
© Copyright Policy
Related In: Results  -  Collection

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Figure 3: Real-time PCR kidney ABCG5 mRNA relative expression in saline- control (SC), saline-training (ST), Baneh-control (BC), and Baneh-training (BT) wild-type female rats. Wild-type female rats data expressed as mean ± SEM. Each column is assigned to one group and 5 rats per each group) **,ST vs BT, (P < 0.003) +, BT vs BC , (P < 0.015)
Mentions: ABCG5 relative gene expression in the liver, small intestine and kidney were determined in female rats. Data analysis revealed a significant difference in liver ABCG5 mRNA relative abundance between groups (F=14.50, P<0.001) (Figure 1). Using a suitable post hoc test, it was shown that the liver relative expression of ABCG5 was higher in ST group (2.2813±0.2285) when compared with other groups at the end of the experiment (Figure 1). Also, a significant difference was found in the small intestine relative mRNA expression of ABCG5 at the end of treadmill running program (F=8.158, P<0.003). In this regard, the ABCG5 mRNA relative abundance was lower in Baneh treated animals (1.0219±0.21305) when compared with saline-treated groups (1.4928±0.14083) (Figure 2). Considering the kidneys, the same results as the liver and intestine have been observed (F=17.61, P<0.001) (Figure 3). The levels of plasma HDL-c were significantly (P<0.05) lower in Baneh-treated animals when compare with their counterparts (Figure 4). There were no significant changes in plasma TG and TC concentrations, (Figure 5 and 6). Collectively, it should be noted that training groups, particularly saline-trained tissues had higher levels of ABCG5 gene expression than other groups.

Bottom Line: The current findings indicate that the Baneh-treated tissues had significantly lower levels of ABCG5 gene expression in the liver, small intestine, and kidneys (P< 0.001, P< 0.003, P< 0.001, respectively), when compared with saline-treated tissues.A lower level of HDL-c but not triglyceride (TG) and total cholesterol (TC) levels were found in Baneh-treated animals at rest.Exercise training increases ABCG5 relative gene expression in the liver, small intestine and kidney tissues; therefore exercise training may adjust the reduction of ABCG5 relative gene expression in Baneh-training group.

View Article: PubMed Central - PubMed

Affiliation: Exercise Biochemistry Division, Faculty of Physical Education and Sport Science, University of Mazandaran, Baboulsar, Mazandaran, Iran.

ABSTRACT

Objective(s): ABC transporters comprise a large family of transmembrane proteins that use the energy provided by ATP hydrolysis to translocate a variety of substrates across biological membranes. All members of the human ABCG subfamily, except for ABCG2, are cholesterol-transporter. The aim of this study was to determine the liver, the small intestine and kidney ABCG5 relative gene expression in response to treadmill-running training in female rats.

Materials and methods: Twenty Wistar rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned to saline-control (SC), saline-training (ST), and Baneh-control (BC), and Baneh-training (BT) groups. Training groups did the exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day for eight weeks (5 days/week). Rats were fed orally, with Baneh extraction and saline for six weeks. The two-way ANOVA was employed for statistical analysis. ABCG5 relative gene expression was detected by Real-time PCR method.

Results: The current findings indicate that the Baneh-treated tissues had significantly lower levels of ABCG5 gene expression in the liver, small intestine, and kidneys (P< 0.001, P< 0.003, P< 0.001, respectively), when compared with saline-treated tissues. However, a higher level of gene expression was observed in exercise groups. A lower level of HDL-c but not triglyceride (TG) and total cholesterol (TC) levels were found in Baneh-treated animals at rest.

Conclusion: Exercise training increases ABCG5 relative gene expression in the liver, small intestine and kidney tissues; therefore exercise training may adjust the reduction of ABCG5 relative gene expression in Baneh-training group.

No MeSH data available.


Related in: MedlinePlus