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Prenatal morphine exposure reduces pyramidal neurons in CA1, CA2 and CA3 subfields of mice hippocampus.

Ghafari S, Golalipour MJ - Iran J Basic Med Sci (2014)

Bottom Line: Quantitative computer-assisted morphometric study was done on hippocampus.The number of pyramidal neurons in CA1, CA2 and CA3 in treated groups was significantly reduced in postnatal day 18 and 32 (P18, P32) compared to control groups (P<0.05).The mean thickness of the stratum pyramidal layer was decreased in the treated groups in comparison with controls (P<0.05), whereas the mean thickness of the stratum oriens, stratum radiatum and stratum lacunosum-moleculare in CA1 field and stratum oriens, stratum lucidum, stratum radiatum and stratum lacunosum-moleculare in CA3 were significantly increased in morphine treated group in comparison with controls (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran.

ABSTRACT

Objective(s): This study was carried out to evaluate the effect of maternal morphine exposure during gestational and lactation period on pyramidal neurons of hippocampus in 18 and 32 day mice offspring.

Materials and methods: Thirty female mice were randomly allocated into cases and controls. In case group, animals received morphine sulfate 10 mg/kg.body weight intraperitoneally during 7 days before mating, gestational period (GD 0-21), 18 and 32 days after delivery in the experimental groups. The control animals received an equivalent volume of normal saline. Cerebrum of six offsprings in each group was removed and stained with cresyl violet and a monoclonal antibody NeuN for immunohistochemical detection of surviving pyramidal neurons. Quantitative computer-assisted morphometric study was done on hippocampus.

Results: The number of pyramidal neurons in CA1, CA2 and CA3 in treated groups was significantly reduced in postnatal day 18 and 32 (P18, P32) compared to control groups (P<0.05). The mean thickness of the stratum pyramidal layer was decreased in the treated groups in comparison with controls (P<0.05), whereas the mean thickness of the stratum oriens, stratum radiatum and stratum lacunosum-moleculare in CA1 field and stratum oriens, stratum lucidum, stratum radiatum and stratum lacunosum-moleculare in CA3 were significantly increased in morphine treated group in comparison with controls (P<0.05).

Conclusion: Morphine administration before and during pregnancy and during lactation period causes pyramidal neurons loss in 18 and 32 days old infant mice.

No MeSH data available.


Related in: MedlinePlus

The mean number of pyramidal cells in (a) hippocampal CA1, (b) CA2 and (c) CA3 subfield in P18 and P32 mice of control and morphine sulphate treated mothers. The cells were expressed as the numb er of pyramidal cells per 10000 μm2, (results are means± SEM, *Compared with controls P<0.05, n=6)
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Figure 3: The mean number of pyramidal cells in (a) hippocampal CA1, (b) CA2 and (c) CA3 subfield in P18 and P32 mice of control and morphine sulphate treated mothers. The cells were expressed as the numb er of pyramidal cells per 10000 μm2, (results are means± SEM, *Compared with controls P<0.05, n=6)

Mentions: In each postnatal mouse, ten similar sections of anterior to posterior of CA1, CA2 and CA3 subfields of hippocampus were selected and images were taken and analyzed by Olympus BX 51 microscope and DP12 digital camera attached to OLYSIA autobioreport software (Olympus Optical, Co. LTD, Japan). The pyramidal cells density was evaluated by counting number of pyramidal cells per 10000 μm2 area of pyramidal layer of CA1, CA2 and CA3 subfield in 1000X magnification. The thickness of layers of hippocampus in CA1 and CA2 included stratum oriens (So), stratum pyramidal (Sp), stratum radiatum (Sr) and stratum lacunosum-moleculare (Slm) and in CA3 field included So, Sp, Sr, Slm and stratum lucidum (Slu) were obtained from 400X magnification (Figures 1-3).


Prenatal morphine exposure reduces pyramidal neurons in CA1, CA2 and CA3 subfields of mice hippocampus.

Ghafari S, Golalipour MJ - Iran J Basic Med Sci (2014)

The mean number of pyramidal cells in (a) hippocampal CA1, (b) CA2 and (c) CA3 subfield in P18 and P32 mice of control and morphine sulphate treated mothers. The cells were expressed as the numb er of pyramidal cells per 10000 μm2, (results are means± SEM, *Compared with controls P<0.05, n=6)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016685&req=5

Figure 3: The mean number of pyramidal cells in (a) hippocampal CA1, (b) CA2 and (c) CA3 subfield in P18 and P32 mice of control and morphine sulphate treated mothers. The cells were expressed as the numb er of pyramidal cells per 10000 μm2, (results are means± SEM, *Compared with controls P<0.05, n=6)
Mentions: In each postnatal mouse, ten similar sections of anterior to posterior of CA1, CA2 and CA3 subfields of hippocampus were selected and images were taken and analyzed by Olympus BX 51 microscope and DP12 digital camera attached to OLYSIA autobioreport software (Olympus Optical, Co. LTD, Japan). The pyramidal cells density was evaluated by counting number of pyramidal cells per 10000 μm2 area of pyramidal layer of CA1, CA2 and CA3 subfield in 1000X magnification. The thickness of layers of hippocampus in CA1 and CA2 included stratum oriens (So), stratum pyramidal (Sp), stratum radiatum (Sr) and stratum lacunosum-moleculare (Slm) and in CA3 field included So, Sp, Sr, Slm and stratum lucidum (Slu) were obtained from 400X magnification (Figures 1-3).

Bottom Line: Quantitative computer-assisted morphometric study was done on hippocampus.The number of pyramidal neurons in CA1, CA2 and CA3 in treated groups was significantly reduced in postnatal day 18 and 32 (P18, P32) compared to control groups (P<0.05).The mean thickness of the stratum pyramidal layer was decreased in the treated groups in comparison with controls (P<0.05), whereas the mean thickness of the stratum oriens, stratum radiatum and stratum lacunosum-moleculare in CA1 field and stratum oriens, stratum lucidum, stratum radiatum and stratum lacunosum-moleculare in CA3 were significantly increased in morphine treated group in comparison with controls (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran.

ABSTRACT

Objective(s): This study was carried out to evaluate the effect of maternal morphine exposure during gestational and lactation period on pyramidal neurons of hippocampus in 18 and 32 day mice offspring.

Materials and methods: Thirty female mice were randomly allocated into cases and controls. In case group, animals received morphine sulfate 10 mg/kg.body weight intraperitoneally during 7 days before mating, gestational period (GD 0-21), 18 and 32 days after delivery in the experimental groups. The control animals received an equivalent volume of normal saline. Cerebrum of six offsprings in each group was removed and stained with cresyl violet and a monoclonal antibody NeuN for immunohistochemical detection of surviving pyramidal neurons. Quantitative computer-assisted morphometric study was done on hippocampus.

Results: The number of pyramidal neurons in CA1, CA2 and CA3 in treated groups was significantly reduced in postnatal day 18 and 32 (P18, P32) compared to control groups (P<0.05). The mean thickness of the stratum pyramidal layer was decreased in the treated groups in comparison with controls (P<0.05), whereas the mean thickness of the stratum oriens, stratum radiatum and stratum lacunosum-moleculare in CA1 field and stratum oriens, stratum lucidum, stratum radiatum and stratum lacunosum-moleculare in CA3 were significantly increased in morphine treated group in comparison with controls (P<0.05).

Conclusion: Morphine administration before and during pregnancy and during lactation period causes pyramidal neurons loss in 18 and 32 days old infant mice.

No MeSH data available.


Related in: MedlinePlus