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Inhibitory effect of allicin and garlic extracts on growth of cultured hyphae.

Aala F, Yusuf UK, Nulit R, Rezaie S - Iran J Basic Med Sci (2014)

Bottom Line: Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported.The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Mycology & Parasitology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Kurdistan, Iran.

ABSTRACT

Objective(s): Trichophyton rubrum (T. rubrum) is one of the most common dermatophytes worldwide. This fungus invaded skin appendages of humans and animals. Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported. Besides, using some plant extracts have been indicated in herbal medicine as an alternative treatment of these fungal infections. The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.

Materials and methods: This study was carried out to observe the morphological changes of T. rubrum treated with allicin as well as aqueous garlic extract using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Results: SEM surveys, showed that hypha treated with allicin has rough and granular like surface, abnormal and irregularly-shape. However, hypha treated with garlic extract had rough and fluffy surface and also irregularly-shape. TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

Conclusion: The present study revealed that pure allicin (6.25 µg/ml and 12.5 µg/ml) is more efficient in inhibition of the growth in hyphal cells compare to the garlic extract (2 mg/ml and 4 mg/ml) and they could be used as alternatives in treatment of dermatophytosis.

No MeSH data available.


Related in: MedlinePlus

TEM studies of a longitudinal section of Trichophyton rubrum. a. Normal untreated hypha showing typical cell wall, cell membrane and organelles. b. Hyphae treated with allicin (12.5 µg/ml) showing disintegration and deterioration of cytoplasm, break down of the cell membrane and cell wall, and collapse of hyphae. c. Hyphae treated with garlic extract (4 mg/ml) indicating degradation and dissolution of cytoplasm components, demolition of the cell wall and cell membrane, and hyphae appeared to break. Scale bar=0.5 µm
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Figure 4: TEM studies of a longitudinal section of Trichophyton rubrum. a. Normal untreated hypha showing typical cell wall, cell membrane and organelles. b. Hyphae treated with allicin (12.5 µg/ml) showing disintegration and deterioration of cytoplasm, break down of the cell membrane and cell wall, and collapse of hyphae. c. Hyphae treated with garlic extract (4 mg/ml) indicating degradation and dissolution of cytoplasm components, demolition of the cell wall and cell membrane, and hyphae appeared to break. Scale bar=0.5 µm

Mentions: TEM results of the treatment of T. rubrum isolates with allicin and garlic extract are shown in Figure 4. Micrograph exhibits normal/untreated hypha with typical cell wall, cell membrane and organelles. In contrast, hyphae treated with allicin (12.5 µg/ml) showed disintegration and deteriora-tion of cytoplasm parts, as well as breakdown of the cell membrane and cell wall, and collapse hyphae. Besides, hyphae treated with garlic extract (4 mg/ml) exhibit degradation and dissolution of cytoplasm components in addition to destruction of cell wall and cell membrane. Hyphae appeared to break in this condition (Figure 4).


Inhibitory effect of allicin and garlic extracts on growth of cultured hyphae.

Aala F, Yusuf UK, Nulit R, Rezaie S - Iran J Basic Med Sci (2014)

TEM studies of a longitudinal section of Trichophyton rubrum. a. Normal untreated hypha showing typical cell wall, cell membrane and organelles. b. Hyphae treated with allicin (12.5 µg/ml) showing disintegration and deterioration of cytoplasm, break down of the cell membrane and cell wall, and collapse of hyphae. c. Hyphae treated with garlic extract (4 mg/ml) indicating degradation and dissolution of cytoplasm components, demolition of the cell wall and cell membrane, and hyphae appeared to break. Scale bar=0.5 µm
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016684&req=5

Figure 4: TEM studies of a longitudinal section of Trichophyton rubrum. a. Normal untreated hypha showing typical cell wall, cell membrane and organelles. b. Hyphae treated with allicin (12.5 µg/ml) showing disintegration and deterioration of cytoplasm, break down of the cell membrane and cell wall, and collapse of hyphae. c. Hyphae treated with garlic extract (4 mg/ml) indicating degradation and dissolution of cytoplasm components, demolition of the cell wall and cell membrane, and hyphae appeared to break. Scale bar=0.5 µm
Mentions: TEM results of the treatment of T. rubrum isolates with allicin and garlic extract are shown in Figure 4. Micrograph exhibits normal/untreated hypha with typical cell wall, cell membrane and organelles. In contrast, hyphae treated with allicin (12.5 µg/ml) showed disintegration and deteriora-tion of cytoplasm parts, as well as breakdown of the cell membrane and cell wall, and collapse hyphae. Besides, hyphae treated with garlic extract (4 mg/ml) exhibit degradation and dissolution of cytoplasm components in addition to destruction of cell wall and cell membrane. Hyphae appeared to break in this condition (Figure 4).

Bottom Line: Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported.The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Mycology & Parasitology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Kurdistan, Iran.

ABSTRACT

Objective(s): Trichophyton rubrum (T. rubrum) is one of the most common dermatophytes worldwide. This fungus invaded skin appendages of humans and animals. Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported. Besides, using some plant extracts have been indicated in herbal medicine as an alternative treatment of these fungal infections. The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.

Materials and methods: This study was carried out to observe the morphological changes of T. rubrum treated with allicin as well as aqueous garlic extract using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Results: SEM surveys, showed that hypha treated with allicin has rough and granular like surface, abnormal and irregularly-shape. However, hypha treated with garlic extract had rough and fluffy surface and also irregularly-shape. TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

Conclusion: The present study revealed that pure allicin (6.25 µg/ml and 12.5 µg/ml) is more efficient in inhibition of the growth in hyphal cells compare to the garlic extract (2 mg/ml and 4 mg/ml) and they could be used as alternatives in treatment of dermatophytosis.

No MeSH data available.


Related in: MedlinePlus