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Inhibitory effect of allicin and garlic extracts on growth of cultured hyphae.

Aala F, Yusuf UK, Nulit R, Rezaie S - Iran J Basic Med Sci (2014)

Bottom Line: Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported.The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Mycology & Parasitology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Kurdistan, Iran.

ABSTRACT

Objective(s): Trichophyton rubrum (T. rubrum) is one of the most common dermatophytes worldwide. This fungus invaded skin appendages of humans and animals. Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported. Besides, using some plant extracts have been indicated in herbal medicine as an alternative treatment of these fungal infections. The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.

Materials and methods: This study was carried out to observe the morphological changes of T. rubrum treated with allicin as well as aqueous garlic extract using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Results: SEM surveys, showed that hypha treated with allicin has rough and granular like surface, abnormal and irregularly-shape. However, hypha treated with garlic extract had rough and fluffy surface and also irregularly-shape. TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

Conclusion: The present study revealed that pure allicin (6.25 µg/ml and 12.5 µg/ml) is more efficient in inhibition of the growth in hyphal cells compare to the garlic extract (2 mg/ml and 4 mg/ml) and they could be used as alternatives in treatment of dermatophytosis.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrographs of Trichophyton. rubrum a. typical hypha was recognized by smooth like surface b.T. rubrum treated with allicin (12.5 µg/ml) showing hyphae with rough and granular like surface c.T. rubrum treated with garlic extract (4 mg/ml) showing hyphae with rough and fluffy surface
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Figure 2: Scanning electron micrographs of Trichophyton. rubrum a. typical hypha was recognized by smooth like surface b.T. rubrum treated with allicin (12.5 µg/ml) showing hyphae with rough and granular like surface c.T. rubrum treated with garlic extract (4 mg/ml) showing hyphae with rough and fluffy surface

Mentions: Scanning electron micrographs of T. rubrum are shown in Figure 2. The comparison between normal and treated (allicin and garlic extract) hypae showed smooth walls in T. rubrum normal hyphae in SEM observation. However, hyphae which were treated with allicin (12.5 µg/ml) were found with rough and granular like surface. Also, SEM observation revealed rough and fluffy surface for hyphae which were treated with 4 mg/ ml of garlic extract (Figure 2).


Inhibitory effect of allicin and garlic extracts on growth of cultured hyphae.

Aala F, Yusuf UK, Nulit R, Rezaie S - Iran J Basic Med Sci (2014)

Scanning electron micrographs of Trichophyton. rubrum a. typical hypha was recognized by smooth like surface b.T. rubrum treated with allicin (12.5 µg/ml) showing hyphae with rough and granular like surface c.T. rubrum treated with garlic extract (4 mg/ml) showing hyphae with rough and fluffy surface
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016684&req=5

Figure 2: Scanning electron micrographs of Trichophyton. rubrum a. typical hypha was recognized by smooth like surface b.T. rubrum treated with allicin (12.5 µg/ml) showing hyphae with rough and granular like surface c.T. rubrum treated with garlic extract (4 mg/ml) showing hyphae with rough and fluffy surface
Mentions: Scanning electron micrographs of T. rubrum are shown in Figure 2. The comparison between normal and treated (allicin and garlic extract) hypae showed smooth walls in T. rubrum normal hyphae in SEM observation. However, hyphae which were treated with allicin (12.5 µg/ml) were found with rough and granular like surface. Also, SEM observation revealed rough and fluffy surface for hyphae which were treated with 4 mg/ ml of garlic extract (Figure 2).

Bottom Line: Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported.The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Mycology & Parasitology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Kurdistan, Iran.

ABSTRACT

Objective(s): Trichophyton rubrum (T. rubrum) is one of the most common dermatophytes worldwide. This fungus invaded skin appendages of humans and animals. Recently, resistance to antifungal drugs as well as appearance of side effects due to indication of these kinds of antibiotics has been reported. Besides, using some plant extracts have been indicated in herbal medicine as an alternative treatment of these fungal infections. The aim of this study was to investigate the effects of Garlic (Allium sativum) and pure allicin on the growth of hypha in T. rubrum using Electron miscroscopy.

Materials and methods: This study was carried out to observe the morphological changes of T. rubrum treated with allicin as well as aqueous garlic extract using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Results: SEM surveys, showed that hypha treated with allicin has rough and granular like surface, abnormal and irregularly-shape. However, hypha treated with garlic extract had rough and fluffy surface and also irregularly-shape. TEM studies also found that hypha treated with allicin displays disintegration of cytoplasm, breaking down in cell membrane and the cell wall, and collapsing of hypha, meanwhile hypha treated with garlic extract exhibiting degradation and dissolution of cytoplasm components, demolition of cell wall and cell membrane, and hypha appeared to break.

Conclusion: The present study revealed that pure allicin (6.25 µg/ml and 12.5 µg/ml) is more efficient in inhibition of the growth in hyphal cells compare to the garlic extract (2 mg/ml and 4 mg/ml) and they could be used as alternatives in treatment of dermatophytosis.

No MeSH data available.


Related in: MedlinePlus