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Glucose-regulated protein 78 and heparanase expression in oral squamous cell carcinoma: correlations and prognostic significance.

Xia F, Xu JC, Zhang P, Zhang YY, Zhang QW, Chao ZH, Wang F - World J Surg Oncol (2014)

Bottom Line: Patients with OSCC showed a statistically significant higher prevalence of GRP78 and HPA expression than normal oral tissues.HPA expression was found to be positively correlated with GRP78 expression.GRP78 and HPA are biomarkers that may have the potential to guide the treatment of oral cancer patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, No 287, Chang-Huai Road, Bengbu 233000, Anhui, People's Republic of China. xjch9999@163.com.

ABSTRACT

Background: The aim of the present study was to investigate the expression of glucose-related protein 78 (GRP78) and heparanase (HPA) in oral squamous cell carcinoma (OSCC) and their relationship with clinicopathological parameters and potential implications for survival.

Methods: A total of 46 patients with OSCC and 10 normal individuals were recruited for the study. GRP78 and HPA expression were determined in the lesion tissues using immunohistochemical analysis. The correlation between GRP78 and HPA was assessed using the Spearman correlation analysis. The associations of GRP78 and HPA with clinicopathological characteristics and survival were examined using the x2-test, Kaplan-Meier, or Cox regression.

Results: Patients with OSCC showed a statistically significant higher prevalence of GRP78 and HPA expression than normal oral tissues. GRP78 and HPA expression was positively correlated with size, TNM stage, histological grade, lymphatic metastasis, and distant metastasis in OSCC patients. GRP78 expression was also positively correlated with HPA expression. Positive GRP78 and HPA expression was inversely correlated with survival in OSCC patients.

Conclusions: HPA expression was found to be positively correlated with GRP78 expression. GRP78 and HPA are biomarkers that may have the potential to guide the treatment of oral cancer patients.

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Related in: MedlinePlus

Immunohistochemistry staining patterns of GRP78 and HPA expressions in normal tissues and OSCC. (A) GRP78 expression in OSCC and normal tissues by immunostaining (×400). A-1: GRP78 in normal tissues: no expression; A-2: GRP78 in OSCC tissues: negative expression; A-3: GRP78 in OSCC tissues: positive expression. (B) HPA expression in OSCC and normal tissues by immunostaining (c × 400). B-1: HPA in normal tissues: no expression; B-2: HPA in OSCC tissues: negative expression; B-3: HPA in OSCC tissues: positive expression. GRP78, glucose-related protein 78; HPA, heparanase; OSCC, oral squamous cell carcinoma.
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Figure 1: Immunohistochemistry staining patterns of GRP78 and HPA expressions in normal tissues and OSCC. (A) GRP78 expression in OSCC and normal tissues by immunostaining (×400). A-1: GRP78 in normal tissues: no expression; A-2: GRP78 in OSCC tissues: negative expression; A-3: GRP78 in OSCC tissues: positive expression. (B) HPA expression in OSCC and normal tissues by immunostaining (c × 400). B-1: HPA in normal tissues: no expression; B-2: HPA in OSCC tissues: negative expression; B-3: HPA in OSCC tissues: positive expression. GRP78, glucose-related protein 78; HPA, heparanase; OSCC, oral squamous cell carcinoma.

Mentions: Clinical samples from 10 normal individuals and 46 OSCC patients were stained to confirm that GRP78 and HPA expression differed between these two groups. GRP78 was expressed in the cytoplasm in all OSCC samples (Figure 1). No GRP78 was expressed in any of the normal oral mucosa samples. In this way, there was a significant difference in the rate of expression of GRP78 between the OSCC group and the control group (P = 0.003). Similarly, HPA-positive expression signals were observed in 19 of 46 OSCC samples, but faint staining signals were observed in addition to the more commonly observed cytoplasmic staining. HPA was also found to localize in the nucleus and cytomembrane (Figure 1). There was a significant difference in the rate of expression of HPA between the OSCC group and the control group (P = 0.033).


Glucose-regulated protein 78 and heparanase expression in oral squamous cell carcinoma: correlations and prognostic significance.

Xia F, Xu JC, Zhang P, Zhang YY, Zhang QW, Chao ZH, Wang F - World J Surg Oncol (2014)

Immunohistochemistry staining patterns of GRP78 and HPA expressions in normal tissues and OSCC. (A) GRP78 expression in OSCC and normal tissues by immunostaining (×400). A-1: GRP78 in normal tissues: no expression; A-2: GRP78 in OSCC tissues: negative expression; A-3: GRP78 in OSCC tissues: positive expression. (B) HPA expression in OSCC and normal tissues by immunostaining (c × 400). B-1: HPA in normal tissues: no expression; B-2: HPA in OSCC tissues: negative expression; B-3: HPA in OSCC tissues: positive expression. GRP78, glucose-related protein 78; HPA, heparanase; OSCC, oral squamous cell carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016628&req=5

Figure 1: Immunohistochemistry staining patterns of GRP78 and HPA expressions in normal tissues and OSCC. (A) GRP78 expression in OSCC and normal tissues by immunostaining (×400). A-1: GRP78 in normal tissues: no expression; A-2: GRP78 in OSCC tissues: negative expression; A-3: GRP78 in OSCC tissues: positive expression. (B) HPA expression in OSCC and normal tissues by immunostaining (c × 400). B-1: HPA in normal tissues: no expression; B-2: HPA in OSCC tissues: negative expression; B-3: HPA in OSCC tissues: positive expression. GRP78, glucose-related protein 78; HPA, heparanase; OSCC, oral squamous cell carcinoma.
Mentions: Clinical samples from 10 normal individuals and 46 OSCC patients were stained to confirm that GRP78 and HPA expression differed between these two groups. GRP78 was expressed in the cytoplasm in all OSCC samples (Figure 1). No GRP78 was expressed in any of the normal oral mucosa samples. In this way, there was a significant difference in the rate of expression of GRP78 between the OSCC group and the control group (P = 0.003). Similarly, HPA-positive expression signals were observed in 19 of 46 OSCC samples, but faint staining signals were observed in addition to the more commonly observed cytoplasmic staining. HPA was also found to localize in the nucleus and cytomembrane (Figure 1). There was a significant difference in the rate of expression of HPA between the OSCC group and the control group (P = 0.033).

Bottom Line: Patients with OSCC showed a statistically significant higher prevalence of GRP78 and HPA expression than normal oral tissues.HPA expression was found to be positively correlated with GRP78 expression.GRP78 and HPA are biomarkers that may have the potential to guide the treatment of oral cancer patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, No 287, Chang-Huai Road, Bengbu 233000, Anhui, People's Republic of China. xjch9999@163.com.

ABSTRACT

Background: The aim of the present study was to investigate the expression of glucose-related protein 78 (GRP78) and heparanase (HPA) in oral squamous cell carcinoma (OSCC) and their relationship with clinicopathological parameters and potential implications for survival.

Methods: A total of 46 patients with OSCC and 10 normal individuals were recruited for the study. GRP78 and HPA expression were determined in the lesion tissues using immunohistochemical analysis. The correlation between GRP78 and HPA was assessed using the Spearman correlation analysis. The associations of GRP78 and HPA with clinicopathological characteristics and survival were examined using the x2-test, Kaplan-Meier, or Cox regression.

Results: Patients with OSCC showed a statistically significant higher prevalence of GRP78 and HPA expression than normal oral tissues. GRP78 and HPA expression was positively correlated with size, TNM stage, histological grade, lymphatic metastasis, and distant metastasis in OSCC patients. GRP78 expression was also positively correlated with HPA expression. Positive GRP78 and HPA expression was inversely correlated with survival in OSCC patients.

Conclusions: HPA expression was found to be positively correlated with GRP78 expression. GRP78 and HPA are biomarkers that may have the potential to guide the treatment of oral cancer patients.

Show MeSH
Related in: MedlinePlus