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A phylogeny for the pomatiopsidae (Gastropoda: Rissooidea): a resource for taxonomic, parasitological and biodiversity studies.

Liu L, Huo GN, He HB, Zhou B, Attwood SW - BMC Evol. Biol. (2014)

Bottom Line: Consequently, the aim of the study was to collect DNA-sequence data for as many pomatiopsid taxa as possible, as a first step in providing a resource for identification of epidemiologically significant species (by non-malacologists), for use in resolving taxonomic confusion and for testing phylogeographical hypotheses.The molecular dates and phylogenetic estimates in this study are consistent with an Australasian origin for the Pomatiopsidae and an East to West radiation via Oligocene Borneo-Philippines island hopping to Japan and then China (Triculinae arising mid-Miocene in Southeast China), and less so with a triculine origin in Tibet.The lack of monophyly in the medically important genera and indications of taxonomic inaccuracies, call for further work to identify epidemiologically significant taxa (e.g., Halewisia may be potential hosts for Schistosoma mekongi) and highlight the need for surveys to determine the true biodiversity of the Triculinae.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, 1 KeYuan 4 Lu, Chengdu, Sichuan 610041, People's Republic of China. swahuaxi@yahoo.com.

ABSTRACT

Background: The Pomatiopsidae are reported from northern India into southern China and Southeast Asia, with two sub-families, the Pomatiopsinae (which include freshwater, amphibious, terrestrial and marine species) and the freshwater Triculinae. Both include species acting as intermediate host for species of the blood-fluke Schistosoma which cause a public health problem in East Asia. Also, with around 120 species, triculine biodiversity exceeds that of any other endemic freshwater molluscan fauna. Nevertheless, the origins of the Pomatiopsidae, the factors driving such a diverse radiation and aspects of their co-evolution with Schistosoma are not fully understood. Many taxonomic questions remain; there are problems identifying medically relevant species. The predicted range is mostly unsurveyed and the true biodiversity of the family is underestimated. Consequently, the aim of the study was to collect DNA-sequence data for as many pomatiopsid taxa as possible, as a first step in providing a resource for identification of epidemiologically significant species (by non-malacologists), for use in resolving taxonomic confusion and for testing phylogeographical hypotheses.

Results: The evolutionary radiation of the Triculinae was shown to have been rapid and mostly post late Miocene. Molecular dating indicated that the radiation of these snails was driven first by the uplift of the Himalaya and onset of a monsoon system, and then by late-Pliocene global warming. The status of Erhaia as Anmicolidae is supported. The genera Tricula and Neotricula are shown to be non-monophyletic and the tribe Jullieniini may be polyphyletic (based on convergent characters). Triculinae from northern Vietnam could be derived from Gammatricula of Fujian/Yunnan, China.

Conclusions: The molecular dates and phylogenetic estimates in this study are consistent with an Australasian origin for the Pomatiopsidae and an East to West radiation via Oligocene Borneo-Philippines island hopping to Japan and then China (Triculinae arising mid-Miocene in Southeast China), and less so with a triculine origin in Tibet. The lack of monophyly in the medically important genera and indications of taxonomic inaccuracies, call for further work to identify epidemiologically significant taxa (e.g., Halewisia may be potential hosts for Schistosoma mekongi) and highlight the need for surveys to determine the true biodiversity of the Triculinae.

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Maximum clade credibility tree estimated by Beast. Ultrametric tree estimated using Beast with “Recent” dating priors (see Hypotheses testing in Methods) and 486540000 generations after burnin. Clade credibilities are shown for each node on the tree. No outgroup was specified, but this tree has been rooted at Lithoglyphus naticoides. Colour scheme: Lacunopsini are classed with Triculini.
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Figure 5: Maximum clade credibility tree estimated by Beast. Ultrametric tree estimated using Beast with “Recent” dating priors (see Hypotheses testing in Methods) and 486540000 generations after burnin. Clade credibilities are shown for each node on the tree. No outgroup was specified, but this tree has been rooted at Lithoglyphus naticoides. Colour scheme: Lacunopsini are classed with Triculini.

Mentions: Figure 5 shows the phylogeny estimated by Beast with all three date priors (see Table 2); this is the MCCT (Maximum Clade Credibility Tree) over all 6000 trees sampled post burnin. The posterior probabilities on the clades ranged from 0.21 to 1.0 (78% of nodes showed support values > 0.50). The posterior probability of the tree itself is −10924.1375 ± 0.1375. The relationships within the West China Triculini clade were the least well supported, as was the relationship between the Jullieniini and the Triculini (P = 0.21). The Amnicolidae form a fairly well supported monophyletic group (P = 0.63); the position of Hydrobia is not well supported (P = 0.39) and it is located adjacent to the root of the tree, whereas Bithynia tentaculata is found at the root of the Pomatiopsidae (with good support P = 0.99). The Jullieniinine clade is well supported (P = 0.99), but Hubendickia spiralis is found at the root of the Pomatiopsidae (after Bithynia) and this is well supported (P = 1.0). The West China Triculini appear to have arisen at almost the same time as the Jullieniini, and both clades appear to stem from the Triculini of northern Thailand (although the support for this is weaker P < 0.35). The pachydrobiinine radiation of Sundaland, together with Gammatricula and Jinghongia, form a large well supported (P = 1.0) clade distinct from the Triculini/Jullieniini clade, which appears to have arisen from Chinese and North Vietnam Pachydrobiini. In addition to the position of the Jullieniini, the Beast tree differs from those of RAxML and MrBayes most strikingly in that it shows the Pomatiopsinae to be a monophyletic clade stemming from the base of the triculine clade (P = 0.51). The two species of Oncomelania are also seen to form a monophyletic, though poorly supported, clade (P = 0.27). Additional file 2: Figure S2 provides a 50% majority rule consensus tree for the 6000 sampled trees and serves to highlight uncertain areas in the phylogeny (i.e., where polytomies occur on the tree).


A phylogeny for the pomatiopsidae (Gastropoda: Rissooidea): a resource for taxonomic, parasitological and biodiversity studies.

Liu L, Huo GN, He HB, Zhou B, Attwood SW - BMC Evol. Biol. (2014)

Maximum clade credibility tree estimated by Beast. Ultrametric tree estimated using Beast with “Recent” dating priors (see Hypotheses testing in Methods) and 486540000 generations after burnin. Clade credibilities are shown for each node on the tree. No outgroup was specified, but this tree has been rooted at Lithoglyphus naticoides. Colour scheme: Lacunopsini are classed with Triculini.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4016560&req=5

Figure 5: Maximum clade credibility tree estimated by Beast. Ultrametric tree estimated using Beast with “Recent” dating priors (see Hypotheses testing in Methods) and 486540000 generations after burnin. Clade credibilities are shown for each node on the tree. No outgroup was specified, but this tree has been rooted at Lithoglyphus naticoides. Colour scheme: Lacunopsini are classed with Triculini.
Mentions: Figure 5 shows the phylogeny estimated by Beast with all three date priors (see Table 2); this is the MCCT (Maximum Clade Credibility Tree) over all 6000 trees sampled post burnin. The posterior probabilities on the clades ranged from 0.21 to 1.0 (78% of nodes showed support values > 0.50). The posterior probability of the tree itself is −10924.1375 ± 0.1375. The relationships within the West China Triculini clade were the least well supported, as was the relationship between the Jullieniini and the Triculini (P = 0.21). The Amnicolidae form a fairly well supported monophyletic group (P = 0.63); the position of Hydrobia is not well supported (P = 0.39) and it is located adjacent to the root of the tree, whereas Bithynia tentaculata is found at the root of the Pomatiopsidae (with good support P = 0.99). The Jullieniinine clade is well supported (P = 0.99), but Hubendickia spiralis is found at the root of the Pomatiopsidae (after Bithynia) and this is well supported (P = 1.0). The West China Triculini appear to have arisen at almost the same time as the Jullieniini, and both clades appear to stem from the Triculini of northern Thailand (although the support for this is weaker P < 0.35). The pachydrobiinine radiation of Sundaland, together with Gammatricula and Jinghongia, form a large well supported (P = 1.0) clade distinct from the Triculini/Jullieniini clade, which appears to have arisen from Chinese and North Vietnam Pachydrobiini. In addition to the position of the Jullieniini, the Beast tree differs from those of RAxML and MrBayes most strikingly in that it shows the Pomatiopsinae to be a monophyletic clade stemming from the base of the triculine clade (P = 0.51). The two species of Oncomelania are also seen to form a monophyletic, though poorly supported, clade (P = 0.27). Additional file 2: Figure S2 provides a 50% majority rule consensus tree for the 6000 sampled trees and serves to highlight uncertain areas in the phylogeny (i.e., where polytomies occur on the tree).

Bottom Line: Consequently, the aim of the study was to collect DNA-sequence data for as many pomatiopsid taxa as possible, as a first step in providing a resource for identification of epidemiologically significant species (by non-malacologists), for use in resolving taxonomic confusion and for testing phylogeographical hypotheses.The molecular dates and phylogenetic estimates in this study are consistent with an Australasian origin for the Pomatiopsidae and an East to West radiation via Oligocene Borneo-Philippines island hopping to Japan and then China (Triculinae arising mid-Miocene in Southeast China), and less so with a triculine origin in Tibet.The lack of monophyly in the medically important genera and indications of taxonomic inaccuracies, call for further work to identify epidemiologically significant taxa (e.g., Halewisia may be potential hosts for Schistosoma mekongi) and highlight the need for surveys to determine the true biodiversity of the Triculinae.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, 1 KeYuan 4 Lu, Chengdu, Sichuan 610041, People's Republic of China. swahuaxi@yahoo.com.

ABSTRACT

Background: The Pomatiopsidae are reported from northern India into southern China and Southeast Asia, with two sub-families, the Pomatiopsinae (which include freshwater, amphibious, terrestrial and marine species) and the freshwater Triculinae. Both include species acting as intermediate host for species of the blood-fluke Schistosoma which cause a public health problem in East Asia. Also, with around 120 species, triculine biodiversity exceeds that of any other endemic freshwater molluscan fauna. Nevertheless, the origins of the Pomatiopsidae, the factors driving such a diverse radiation and aspects of their co-evolution with Schistosoma are not fully understood. Many taxonomic questions remain; there are problems identifying medically relevant species. The predicted range is mostly unsurveyed and the true biodiversity of the family is underestimated. Consequently, the aim of the study was to collect DNA-sequence data for as many pomatiopsid taxa as possible, as a first step in providing a resource for identification of epidemiologically significant species (by non-malacologists), for use in resolving taxonomic confusion and for testing phylogeographical hypotheses.

Results: The evolutionary radiation of the Triculinae was shown to have been rapid and mostly post late Miocene. Molecular dating indicated that the radiation of these snails was driven first by the uplift of the Himalaya and onset of a monsoon system, and then by late-Pliocene global warming. The status of Erhaia as Anmicolidae is supported. The genera Tricula and Neotricula are shown to be non-monophyletic and the tribe Jullieniini may be polyphyletic (based on convergent characters). Triculinae from northern Vietnam could be derived from Gammatricula of Fujian/Yunnan, China.

Conclusions: The molecular dates and phylogenetic estimates in this study are consistent with an Australasian origin for the Pomatiopsidae and an East to West radiation via Oligocene Borneo-Philippines island hopping to Japan and then China (Triculinae arising mid-Miocene in Southeast China), and less so with a triculine origin in Tibet. The lack of monophyly in the medically important genera and indications of taxonomic inaccuracies, call for further work to identify epidemiologically significant taxa (e.g., Halewisia may be potential hosts for Schistosoma mekongi) and highlight the need for surveys to determine the true biodiversity of the Triculinae.

Show MeSH
Related in: MedlinePlus