Limits...
Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Guangdong in 2012.

Xiao H, Guan D, Chen R, Chen P, Monagin C, Li W, Su J, Ma C, Zhang W, Ke C - Virol. J. (2013)

Bottom Line: Amplification and sequencing of the VP1 region of enterovirus isolates revealed Echovirus 30 (E30) was the most common isolated serotype (80% of 40 enterovirus strains).For the molecular characterization of the E30 isolates, the VP1 gene sequence of 20 Luoding E30 isolates was compared pairwise using the MegAlign with reference strains from GenBank.Reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences resulted in a monophyletic tree, with seven clustered lineage groups.The study also demonstrated that different E30 variants existed in the local meningitis outbreak.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory for Emergency Pathogen Detection, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong, China. kecw2011@sina.com.

ABSTRACT

Background: Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. An outbreak of aseptic meningitis occurred from May to June, 2012, in Guangdong Province, China. In order to determine the etiologic agent, CSF specimens from 121 children hospitalized for aseptic meningitis at Luoding People's Hospital of Guangdong Province were tested for virus isolation and identification.

Results: Enterovirus RNA was positive in 62.0% of 121 CSF sspecimens by real-time polymerase chain reaction (RT-PCR). Amplification and sequencing of the VP1 region of enterovirus isolates revealed Echovirus 30 (E30) was the most common isolated serotype (80% of 40 enterovirus strains).For the molecular characterization of the E30 isolates, the VP1 gene sequence of 20 Luoding E30 isolates was compared pairwise using the MegAlign with reference strains from GenBank. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the strains differed from those of lineage groups C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences resulted in a monophyletic tree, with seven clustered lineage groups. Most of the isolates were segregated from other lineage groups. Four E30 isolates causing this outbreak aggregated into the Lineage A cluster which was derived from E30 strains that circulated in other regions of China from 2003-2010.

Conclusions: This study demonstrated the Luoding strains were a distinct lineage of E30, and a probable cause of this outbreak. The study also demonstrated that different E30 variants existed in the local meningitis outbreak.

Show MeSH

Related in: MedlinePlus

Phylogenetic analysis of 2012 Luoding isolates and reference strains of E30. Part nucleotide sequences of VP1 (nt 2,627-2,951) were analyzed via the neighbor-joining methods and the tree was constructed using the MEGA (version 4.0, software package). The nucleotide position is relative to the sequence of Bastianni strain of E30 prototype; Luoding E30 strains are marked using the symbol; The bracketed regions indicate Lineage, marked with A, B, C, D, E, F, G.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4016494&req=5

Figure 2: Phylogenetic analysis of 2012 Luoding isolates and reference strains of E30. Part nucleotide sequences of VP1 (nt 2,627-2,951) were analyzed via the neighbor-joining methods and the tree was constructed using the MEGA (version 4.0, software package). The nucleotide position is relative to the sequence of Bastianni strain of E30 prototype; Luoding E30 strains are marked using the symbol; The bracketed regions indicate Lineage, marked with A, B, C, D, E, F, G.

Mentions: In order to analyze the genetic characteristics of E30, the sequences of 20 E30 strains isolated from aseptic meningitis patients were selected (patient information listed in Table 1). The VP1 sequences of 20 isolates were determined and compared with 39 reference strains from GenBank database. The identity of the nucleotide sequence for the VP1 region within these isolates was high (> 90%) in the 324 bp. The 16 nucleotide sequences of 2012EM isolates differed from the Group A lineage of E30 isolated in China between 2003 ~ 2010. Pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the strains differed from those of the isolates of lineage groups C, D, E, F and G (Figure 2). Nucleotide sequences encoding for VP1 (nt 2,627-2,951) were analyzed via the neighbor-joining methods and trees were constructed using the MEGA software package, version 4.0. The reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences of the isolates and the E30 reference strains supported a monophyletic VP1 tree. According to the clusters formed via phylogenetic analysis, E30 was clustered into seven lineage groups. Sixteen isolates were segregated from the other previously reported lineage groups, thus suggesting that the strains were a distinct lineage of E30, and may plausibly have been the major cause of this outbreak.


Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Guangdong in 2012.

Xiao H, Guan D, Chen R, Chen P, Monagin C, Li W, Su J, Ma C, Zhang W, Ke C - Virol. J. (2013)

Phylogenetic analysis of 2012 Luoding isolates and reference strains of E30. Part nucleotide sequences of VP1 (nt 2,627-2,951) were analyzed via the neighbor-joining methods and the tree was constructed using the MEGA (version 4.0, software package). The nucleotide position is relative to the sequence of Bastianni strain of E30 prototype; Luoding E30 strains are marked using the symbol; The bracketed regions indicate Lineage, marked with A, B, C, D, E, F, G.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016494&req=5

Figure 2: Phylogenetic analysis of 2012 Luoding isolates and reference strains of E30. Part nucleotide sequences of VP1 (nt 2,627-2,951) were analyzed via the neighbor-joining methods and the tree was constructed using the MEGA (version 4.0, software package). The nucleotide position is relative to the sequence of Bastianni strain of E30 prototype; Luoding E30 strains are marked using the symbol; The bracketed regions indicate Lineage, marked with A, B, C, D, E, F, G.
Mentions: In order to analyze the genetic characteristics of E30, the sequences of 20 E30 strains isolated from aseptic meningitis patients were selected (patient information listed in Table 1). The VP1 sequences of 20 isolates were determined and compared with 39 reference strains from GenBank database. The identity of the nucleotide sequence for the VP1 region within these isolates was high (> 90%) in the 324 bp. The 16 nucleotide sequences of 2012EM isolates differed from the Group A lineage of E30 isolated in China between 2003 ~ 2010. Pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the strains differed from those of the isolates of lineage groups C, D, E, F and G (Figure 2). Nucleotide sequences encoding for VP1 (nt 2,627-2,951) were analyzed via the neighbor-joining methods and trees were constructed using the MEGA software package, version 4.0. The reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences of the isolates and the E30 reference strains supported a monophyletic VP1 tree. According to the clusters formed via phylogenetic analysis, E30 was clustered into seven lineage groups. Sixteen isolates were segregated from the other previously reported lineage groups, thus suggesting that the strains were a distinct lineage of E30, and may plausibly have been the major cause of this outbreak.

Bottom Line: Amplification and sequencing of the VP1 region of enterovirus isolates revealed Echovirus 30 (E30) was the most common isolated serotype (80% of 40 enterovirus strains).For the molecular characterization of the E30 isolates, the VP1 gene sequence of 20 Luoding E30 isolates was compared pairwise using the MegAlign with reference strains from GenBank.Reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences resulted in a monophyletic tree, with seven clustered lineage groups.The study also demonstrated that different E30 variants existed in the local meningitis outbreak.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory for Emergency Pathogen Detection, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong, China. kecw2011@sina.com.

ABSTRACT

Background: Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. An outbreak of aseptic meningitis occurred from May to June, 2012, in Guangdong Province, China. In order to determine the etiologic agent, CSF specimens from 121 children hospitalized for aseptic meningitis at Luoding People's Hospital of Guangdong Province were tested for virus isolation and identification.

Results: Enterovirus RNA was positive in 62.0% of 121 CSF sspecimens by real-time polymerase chain reaction (RT-PCR). Amplification and sequencing of the VP1 region of enterovirus isolates revealed Echovirus 30 (E30) was the most common isolated serotype (80% of 40 enterovirus strains).For the molecular characterization of the E30 isolates, the VP1 gene sequence of 20 Luoding E30 isolates was compared pairwise using the MegAlign with reference strains from GenBank. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the strains differed from those of lineage groups C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the VP1 nucleotide sequences resulted in a monophyletic tree, with seven clustered lineage groups. Most of the isolates were segregated from other lineage groups. Four E30 isolates causing this outbreak aggregated into the Lineage A cluster which was derived from E30 strains that circulated in other regions of China from 2003-2010.

Conclusions: This study demonstrated the Luoding strains were a distinct lineage of E30, and a probable cause of this outbreak. The study also demonstrated that different E30 variants existed in the local meningitis outbreak.

Show MeSH
Related in: MedlinePlus