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Ocular and neural distribution of feline herpesvirus-1 during active and latent experimental infection in cats.

Townsend WM, Jacobi S, Tai SH, Kiupel M, Wise AG, Maes RK - BMC Vet. Res. (2013)

Bottom Line: The correlation between clinical score and day 30 viral DNA copy number suggests the severity of the acute clinical infection is related to the quantity of latent viral DNA.The histologic response was similar to that seen during HSV-1 or VZV infection.To the author's knowledge this is the first report of FeHV-1 infection involving intraocular structures and autonomic ganglia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, D208 Veterinary Medical Center, 48824-1314 East Lansing, MI, USA. townsenw@purdue.edu.

ABSTRACT

Background: Herpes simplex virus 1 (HSV-1) and varicella zoster virus (VZV) cause extensive intra-ocular and neural infections in humans and are closely related to Felid herpes virus 1 (FeHV-1). We report the extent of intra-ocular replication and the extent and morphological aspects of neural replication during the acute and latent phases of FeHV-1 infection. Juvenile, SPF cats were inoculated with FeHV-1. Additional cats were used as negative controls. Cats were euthanized on days 6, 10, and 30 post-inoculation.

Results: FeHV-1 was isolated from the conjunctiva, cornea, uveal tract, retina, optic nerve, ciliary ganglion (CG), pterygopalatine ganglion (PTPG), trigeminal ganglion (TG), brainstem, visual cortex, cerebellum, and olfactory bulb of infected cats during the acute phase, but not the cranial cervical ganglion (CCG) and optic chiasm. Viral DNA was detected in all tissues during acute infection by a real-time quantitative PCR assay. On day 30, viral DNA was detected in all TG, all CCG, and 2 PTPG. Histologically mild inflammation and ganglion cell loss were noted within the TG during acute, but not latent infection. Using linear regression, a strong correlation existed between clinical score and day 30 viral DNA copy number within the TG.

Conclusions: The correlation between clinical score and day 30 viral DNA copy number suggests the severity of the acute clinical infection is related to the quantity of latent viral DNA. The histologic response was similar to that seen during HSV-1 or VZV infection. To the author's knowledge this is the first report of FeHV-1 infection involving intraocular structures and autonomic ganglia.

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Related in: MedlinePlus

FeHV-1 copy number versus days post inoculation. The average number of copies of FeHV-1 genome per 100 cells was plotted against the days post inoculation. The FeHV-1 copy number decreases over time in most tissues sampled. However, the ciliary ganglia, trigeminal ganglia, cranial cervical ganglia, and optic chiasm had higher levels of virus present at day 10 than at day 6.
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Figure 1: FeHV-1 copy number versus days post inoculation. The average number of copies of FeHV-1 genome per 100 cells was plotted against the days post inoculation. The FeHV-1 copy number decreases over time in most tissues sampled. However, the ciliary ganglia, trigeminal ganglia, cranial cervical ganglia, and optic chiasm had higher levels of virus present at day 10 than at day 6.

Mentions: The PCR results for each individual tissue type and the FeHV-1 DNA copy numbers reported per 100 cells of the homogenized tissues examined are shown in Table 2 and Figure 1. As the number of FeHV-1 genomes/μg of sample DNA was known, the FeHV-1 copy number per 100 cells was calculated based on 260,000 haploid genome equivalents/μg of feline cellular DNA[30]. Individual cell types were not examined. The FeHV-1 copy number per 100 cells was reported to standardize comparisons between the various tissues as different tissues yielded different amounts of DNA. FeHV-1 DNA was not detected by real-time PCR in any of the samples obtained from the control cats. For each group, the total of the clinical scores from Day 0 to the day of euthanasia and the FeHV-1 DNA copy number per 100 cells in the TG were compared by linear regression (Figure 2). The total clinical score and FeHV-1 copy number were strongly correlated (p=0.021, R2=0.9583) for group 3. The clinical score and FeHV-1 copy number were not correlated for groups 1 (p=0.39, R2=0.37) or 2 (p=0.41, R2=0.36).


Ocular and neural distribution of feline herpesvirus-1 during active and latent experimental infection in cats.

Townsend WM, Jacobi S, Tai SH, Kiupel M, Wise AG, Maes RK - BMC Vet. Res. (2013)

FeHV-1 copy number versus days post inoculation. The average number of copies of FeHV-1 genome per 100 cells was plotted against the days post inoculation. The FeHV-1 copy number decreases over time in most tissues sampled. However, the ciliary ganglia, trigeminal ganglia, cranial cervical ganglia, and optic chiasm had higher levels of virus present at day 10 than at day 6.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016492&req=5

Figure 1: FeHV-1 copy number versus days post inoculation. The average number of copies of FeHV-1 genome per 100 cells was plotted against the days post inoculation. The FeHV-1 copy number decreases over time in most tissues sampled. However, the ciliary ganglia, trigeminal ganglia, cranial cervical ganglia, and optic chiasm had higher levels of virus present at day 10 than at day 6.
Mentions: The PCR results for each individual tissue type and the FeHV-1 DNA copy numbers reported per 100 cells of the homogenized tissues examined are shown in Table 2 and Figure 1. As the number of FeHV-1 genomes/μg of sample DNA was known, the FeHV-1 copy number per 100 cells was calculated based on 260,000 haploid genome equivalents/μg of feline cellular DNA[30]. Individual cell types were not examined. The FeHV-1 copy number per 100 cells was reported to standardize comparisons between the various tissues as different tissues yielded different amounts of DNA. FeHV-1 DNA was not detected by real-time PCR in any of the samples obtained from the control cats. For each group, the total of the clinical scores from Day 0 to the day of euthanasia and the FeHV-1 DNA copy number per 100 cells in the TG were compared by linear regression (Figure 2). The total clinical score and FeHV-1 copy number were strongly correlated (p=0.021, R2=0.9583) for group 3. The clinical score and FeHV-1 copy number were not correlated for groups 1 (p=0.39, R2=0.37) or 2 (p=0.41, R2=0.36).

Bottom Line: The correlation between clinical score and day 30 viral DNA copy number suggests the severity of the acute clinical infection is related to the quantity of latent viral DNA.The histologic response was similar to that seen during HSV-1 or VZV infection.To the author's knowledge this is the first report of FeHV-1 infection involving intraocular structures and autonomic ganglia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, D208 Veterinary Medical Center, 48824-1314 East Lansing, MI, USA. townsenw@purdue.edu.

ABSTRACT

Background: Herpes simplex virus 1 (HSV-1) and varicella zoster virus (VZV) cause extensive intra-ocular and neural infections in humans and are closely related to Felid herpes virus 1 (FeHV-1). We report the extent of intra-ocular replication and the extent and morphological aspects of neural replication during the acute and latent phases of FeHV-1 infection. Juvenile, SPF cats were inoculated with FeHV-1. Additional cats were used as negative controls. Cats were euthanized on days 6, 10, and 30 post-inoculation.

Results: FeHV-1 was isolated from the conjunctiva, cornea, uveal tract, retina, optic nerve, ciliary ganglion (CG), pterygopalatine ganglion (PTPG), trigeminal ganglion (TG), brainstem, visual cortex, cerebellum, and olfactory bulb of infected cats during the acute phase, but not the cranial cervical ganglion (CCG) and optic chiasm. Viral DNA was detected in all tissues during acute infection by a real-time quantitative PCR assay. On day 30, viral DNA was detected in all TG, all CCG, and 2 PTPG. Histologically mild inflammation and ganglion cell loss were noted within the TG during acute, but not latent infection. Using linear regression, a strong correlation existed between clinical score and day 30 viral DNA copy number within the TG.

Conclusions: The correlation between clinical score and day 30 viral DNA copy number suggests the severity of the acute clinical infection is related to the quantity of latent viral DNA. The histologic response was similar to that seen during HSV-1 or VZV infection. To the author's knowledge this is the first report of FeHV-1 infection involving intraocular structures and autonomic ganglia.

Show MeSH
Related in: MedlinePlus