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Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SS.

Deng Y, Boon C, Chen S, Lim A, Zhang LH - BMC Microbiol. (2013)

Bottom Line: Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level.Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, Singapore, 138673, Singapore. ydeng@imcb.a-star.edu.sg.

ABSTRACT

Background: Cis-2-dodecenoic acid (BDSF) is well known for its important functions in intraspecies signaling in Burkholderia cenocepacia. Previous work has also established an important role of BDSF in interspecies and inter-kingdom communications. It was identified that BDSF modulates virulence of Pseudomonas aeruginosa. However, how BDSF interferes with virulence of P. aeruginosa is still not clear.

Results: We report here that BDSF mediates the cross-talk between B. cenocepacia and P. aeruginosa through interference with quorum sensing (QS) systems and type III secretion system (T3SS) of P. aeruginosa. Bioassay results revealed that exogenous addition of BDSF not only reduced the transcriptional expression of the regulator encoding gene of QS systems, i.e., lasR, pqsR, and rhlR, but also simultaneously decreased the production of QS signals including 3-oxo-C12-HSL, Pseudomonas quinolone signal (PQS) and C4-HSL, consequently resulting in the down-regulation of biofilm formation and virulence factor production of P. aeruginosa. Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level. Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.

Conclusions: These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

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Related in: MedlinePlus

BDSF attenuates the virulence of P. aeruginosa PA14 in both in vitro and in vivo model. Cytotoxicity was assayed by monitoring LDH released by the HeLa cells infected with a MOI of about 50. Experiments were performed with DMEM medium supplemented with BDSF. The data are the means of three repeats and error bars indicate the standard deviations (A). Zebra-fish model was assayed by testing the survival rate of the fish after infection with P. aeruginosa PA14, without (■) and with (□) treatment of BDSF. PBS containing relevant BDSF was injected in the same way as for a mock control (♦). The experiment was repeated three times, and a representative set of data is shown (B).
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Figure 6: BDSF attenuates the virulence of P. aeruginosa PA14 in both in vitro and in vivo model. Cytotoxicity was assayed by monitoring LDH released by the HeLa cells infected with a MOI of about 50. Experiments were performed with DMEM medium supplemented with BDSF. The data are the means of three repeats and error bars indicate the standard deviations (A). Zebra-fish model was assayed by testing the survival rate of the fish after infection with P. aeruginosa PA14, without (■) and with (□) treatment of BDSF. PBS containing relevant BDSF was injected in the same way as for a mock control (♦). The experiment was repeated three times, and a representative set of data is shown (B).

Mentions: To determine the effect of BDSF on the virulence of P. aeruginosa PA14, HeLa cells were firstly used as the in vitro model. Results indicated that exogenous addition of BDSF significantly decreased the cytotoxicity of P. aeruginosa PA14 to HeLa cell. For 2 hours inoculation, the cytotoxicity was reduced by 41% and 75% with treatment of 5 μM and 25 μM BDSF, respectively (Figure 6A). While for 5 hours inoculation, the reductions were 16% and 73%, respectively (Figure 6A).


Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SS.

Deng Y, Boon C, Chen S, Lim A, Zhang LH - BMC Microbiol. (2013)

BDSF attenuates the virulence of P. aeruginosa PA14 in both in vitro and in vivo model. Cytotoxicity was assayed by monitoring LDH released by the HeLa cells infected with a MOI of about 50. Experiments were performed with DMEM medium supplemented with BDSF. The data are the means of three repeats and error bars indicate the standard deviations (A). Zebra-fish model was assayed by testing the survival rate of the fish after infection with P. aeruginosa PA14, without (■) and with (□) treatment of BDSF. PBS containing relevant BDSF was injected in the same way as for a mock control (♦). The experiment was repeated three times, and a representative set of data is shown (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016476&req=5

Figure 6: BDSF attenuates the virulence of P. aeruginosa PA14 in both in vitro and in vivo model. Cytotoxicity was assayed by monitoring LDH released by the HeLa cells infected with a MOI of about 50. Experiments were performed with DMEM medium supplemented with BDSF. The data are the means of three repeats and error bars indicate the standard deviations (A). Zebra-fish model was assayed by testing the survival rate of the fish after infection with P. aeruginosa PA14, without (■) and with (□) treatment of BDSF. PBS containing relevant BDSF was injected in the same way as for a mock control (♦). The experiment was repeated three times, and a representative set of data is shown (B).
Mentions: To determine the effect of BDSF on the virulence of P. aeruginosa PA14, HeLa cells were firstly used as the in vitro model. Results indicated that exogenous addition of BDSF significantly decreased the cytotoxicity of P. aeruginosa PA14 to HeLa cell. For 2 hours inoculation, the cytotoxicity was reduced by 41% and 75% with treatment of 5 μM and 25 μM BDSF, respectively (Figure 6A). While for 5 hours inoculation, the reductions were 16% and 73%, respectively (Figure 6A).

Bottom Line: Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level.Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, Singapore, 138673, Singapore. ydeng@imcb.a-star.edu.sg.

ABSTRACT

Background: Cis-2-dodecenoic acid (BDSF) is well known for its important functions in intraspecies signaling in Burkholderia cenocepacia. Previous work has also established an important role of BDSF in interspecies and inter-kingdom communications. It was identified that BDSF modulates virulence of Pseudomonas aeruginosa. However, how BDSF interferes with virulence of P. aeruginosa is still not clear.

Results: We report here that BDSF mediates the cross-talk between B. cenocepacia and P. aeruginosa through interference with quorum sensing (QS) systems and type III secretion system (T3SS) of P. aeruginosa. Bioassay results revealed that exogenous addition of BDSF not only reduced the transcriptional expression of the regulator encoding gene of QS systems, i.e., lasR, pqsR, and rhlR, but also simultaneously decreased the production of QS signals including 3-oxo-C12-HSL, Pseudomonas quinolone signal (PQS) and C4-HSL, consequently resulting in the down-regulation of biofilm formation and virulence factor production of P. aeruginosa. Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level. Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.

Conclusions: These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

Show MeSH
Related in: MedlinePlus