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Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SS.

Deng Y, Boon C, Chen S, Lim A, Zhang LH - BMC Microbiol. (2013)

Bottom Line: Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level.Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, Singapore, 138673, Singapore. ydeng@imcb.a-star.edu.sg.

ABSTRACT

Background: Cis-2-dodecenoic acid (BDSF) is well known for its important functions in intraspecies signaling in Burkholderia cenocepacia. Previous work has also established an important role of BDSF in interspecies and inter-kingdom communications. It was identified that BDSF modulates virulence of Pseudomonas aeruginosa. However, how BDSF interferes with virulence of P. aeruginosa is still not clear.

Results: We report here that BDSF mediates the cross-talk between B. cenocepacia and P. aeruginosa through interference with quorum sensing (QS) systems and type III secretion system (T3SS) of P. aeruginosa. Bioassay results revealed that exogenous addition of BDSF not only reduced the transcriptional expression of the regulator encoding gene of QS systems, i.e., lasR, pqsR, and rhlR, but also simultaneously decreased the production of QS signals including 3-oxo-C12-HSL, Pseudomonas quinolone signal (PQS) and C4-HSL, consequently resulting in the down-regulation of biofilm formation and virulence factor production of P. aeruginosa. Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level. Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.

Conclusions: These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

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Inhibitory effect of BDSF on biofilm formation (A), production of extracellular protease (B) and pyocyanin (C) of P. aeruginosa PA14. The data are the means of three repeats and error bars indicate the standard deviations.
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Figure 2: Inhibitory effect of BDSF on biofilm formation (A), production of extracellular protease (B) and pyocyanin (C) of P. aeruginosa PA14. The data are the means of three repeats and error bars indicate the standard deviations.

Mentions: It is well known that QS systems in P. aeruginosa modulate biofilm development, virulence factor production, and antimicrobial resistance [7]. Linking with the finding of the inhibitory activity of BDSF on the QS systems, we then continued to determine the effect of BDSF on the biofilm formation and virulence factor production. As shown in Figure 1A, exogenous addition of BDSF showed no obvious effect on the growth rate of P. aeruginosa PA14; while it remarkably decreased the biofilm formation. Addition of 0.05, 0.1, 0.25 and 0.5 mM BDSF reduced the biofilm formation by 10.2%, 20.2%, 27.9%, and 44.%, respectively (Figure 2A).


Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SS.

Deng Y, Boon C, Chen S, Lim A, Zhang LH - BMC Microbiol. (2013)

Inhibitory effect of BDSF on biofilm formation (A), production of extracellular protease (B) and pyocyanin (C) of P. aeruginosa PA14. The data are the means of three repeats and error bars indicate the standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016476&req=5

Figure 2: Inhibitory effect of BDSF on biofilm formation (A), production of extracellular protease (B) and pyocyanin (C) of P. aeruginosa PA14. The data are the means of three repeats and error bars indicate the standard deviations.
Mentions: It is well known that QS systems in P. aeruginosa modulate biofilm development, virulence factor production, and antimicrobial resistance [7]. Linking with the finding of the inhibitory activity of BDSF on the QS systems, we then continued to determine the effect of BDSF on the biofilm formation and virulence factor production. As shown in Figure 1A, exogenous addition of BDSF showed no obvious effect on the growth rate of P. aeruginosa PA14; while it remarkably decreased the biofilm formation. Addition of 0.05, 0.1, 0.25 and 0.5 mM BDSF reduced the biofilm formation by 10.2%, 20.2%, 27.9%, and 44.%, respectively (Figure 2A).

Bottom Line: Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level.Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, Singapore, 138673, Singapore. ydeng@imcb.a-star.edu.sg.

ABSTRACT

Background: Cis-2-dodecenoic acid (BDSF) is well known for its important functions in intraspecies signaling in Burkholderia cenocepacia. Previous work has also established an important role of BDSF in interspecies and inter-kingdom communications. It was identified that BDSF modulates virulence of Pseudomonas aeruginosa. However, how BDSF interferes with virulence of P. aeruginosa is still not clear.

Results: We report here that BDSF mediates the cross-talk between B. cenocepacia and P. aeruginosa through interference with quorum sensing (QS) systems and type III secretion system (T3SS) of P. aeruginosa. Bioassay results revealed that exogenous addition of BDSF not only reduced the transcriptional expression of the regulator encoding gene of QS systems, i.e., lasR, pqsR, and rhlR, but also simultaneously decreased the production of QS signals including 3-oxo-C12-HSL, Pseudomonas quinolone signal (PQS) and C4-HSL, consequently resulting in the down-regulation of biofilm formation and virulence factor production of P. aeruginosa. Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level. Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models.

Conclusions: These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.

Show MeSH
Related in: MedlinePlus