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Expression of hypoxia-inducible factor-1α and vascular density in mammary adenomas and adenocarcinomas in bitches.

Madej JA, Madej JP, Dziegiel P, Pula B, Nowak M - Acta Vet. Scand. (2013)

Bottom Line: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation.A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004).Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland. marcin.nowak@up.wroc.pl.

ABSTRACT

Background: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.

Results: A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

Conclusion: Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.

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Von Willbrand Factor mean vascular density (vWF MVD) in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001, Mann–Whitney test.
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Figure 4: Von Willbrand Factor mean vascular density (vWF MVD) in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001, Mann–Whitney test.

Mentions: An extremely important and interesting aspect of the study was the examination of a correlation between HIF-1α protein expression and vWF MVD (Figures 1E, 1F). Both in adenomas and in adenocarcinomas the correlation proved to be pronounced and positive. Its level was slightly higher in malignant tumours (adenocarcinomas: r = 0.57; P < 0.05; Figure 3C) than in benign tumours (adenomas r = 0.52; P < 0.05). Mann–Whitney test demonstrated significant differences in vWF MVD between individual malignancy grades (G1 vs. G2 – P = 0.251; G1 vs. G3 – P = 0.098; G2 vs. G3 – P = 0.586) – Figure 4.


Expression of hypoxia-inducible factor-1α and vascular density in mammary adenomas and adenocarcinomas in bitches.

Madej JA, Madej JP, Dziegiel P, Pula B, Nowak M - Acta Vet. Scand. (2013)

Von Willbrand Factor mean vascular density (vWF MVD) in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001, Mann–Whitney test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016321&req=5

Figure 4: Von Willbrand Factor mean vascular density (vWF MVD) in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001, Mann–Whitney test.
Mentions: An extremely important and interesting aspect of the study was the examination of a correlation between HIF-1α protein expression and vWF MVD (Figures 1E, 1F). Both in adenomas and in adenocarcinomas the correlation proved to be pronounced and positive. Its level was slightly higher in malignant tumours (adenocarcinomas: r = 0.57; P < 0.05; Figure 3C) than in benign tumours (adenomas r = 0.52; P < 0.05). Mann–Whitney test demonstrated significant differences in vWF MVD between individual malignancy grades (G1 vs. G2 – P = 0.251; G1 vs. G3 – P = 0.098; G2 vs. G3 – P = 0.586) – Figure 4.

Bottom Line: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation.A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004).Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland. marcin.nowak@up.wroc.pl.

ABSTRACT

Background: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.

Results: A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

Conclusion: Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.

Show MeSH
Related in: MedlinePlus