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Expression of hypoxia-inducible factor-1α and vascular density in mammary adenomas and adenocarcinomas in bitches.

Madej JA, Madej JP, Dziegiel P, Pula B, Nowak M - Acta Vet. Scand. (2013)

Bottom Line: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation.A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004).Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland. marcin.nowak@up.wroc.pl.

ABSTRACT

Background: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.

Results: A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

Conclusion: Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.

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Correlations between expressions of HIF-1α, Ki-67 antigen (A), TUNEL (B) and vWF MVD (C) in the analyzed adenocarcinomas.
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Figure 3: Correlations between expressions of HIF-1α, Ki-67 antigen (A), TUNEL (B) and vWF MVD (C) in the analyzed adenocarcinomas.

Mentions: Tumour cell proliferation was determined by assessing the expression of the Ki-67 antigen (Figure 1C). Similarly, positive correlations were disclosed between tumour’s malignancy grade and expression of Ki-67 proliferation antigen (r = 0.61; P < 0.0001), and between expressions of HIF-1α and Ki-67 (r = 0.43; P < 0.0005; Figure 3A).


Expression of hypoxia-inducible factor-1α and vascular density in mammary adenomas and adenocarcinomas in bitches.

Madej JA, Madej JP, Dziegiel P, Pula B, Nowak M - Acta Vet. Scand. (2013)

Correlations between expressions of HIF-1α, Ki-67 antigen (A), TUNEL (B) and vWF MVD (C) in the analyzed adenocarcinomas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016321&req=5

Figure 3: Correlations between expressions of HIF-1α, Ki-67 antigen (A), TUNEL (B) and vWF MVD (C) in the analyzed adenocarcinomas.
Mentions: Tumour cell proliferation was determined by assessing the expression of the Ki-67 antigen (Figure 1C). Similarly, positive correlations were disclosed between tumour’s malignancy grade and expression of Ki-67 proliferation antigen (r = 0.61; P < 0.0001), and between expressions of HIF-1α and Ki-67 (r = 0.43; P < 0.0005; Figure 3A).

Bottom Line: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation.A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004).Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland. marcin.nowak@up.wroc.pl.

ABSTRACT

Background: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.

Results: A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

Conclusion: Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.

Show MeSH
Related in: MedlinePlus