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Expression of hypoxia-inducible factor-1α and vascular density in mammary adenomas and adenocarcinomas in bitches.

Madej JA, Madej JP, Dziegiel P, Pula B, Nowak M - Acta Vet. Scand. (2013)

Bottom Line: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation.A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004).Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland. marcin.nowak@up.wroc.pl.

ABSTRACT

Background: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.

Results: A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

Conclusion: Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.

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Hypoxia-inducible factor-1α (HIF-1α) expression in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001; ****P < 0.0001, Mann–Whitney test.
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Figure 2: Hypoxia-inducible factor-1α (HIF-1α) expression in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001; ****P < 0.0001, Mann–Whitney test.

Mentions: Expression of HIF-1α protein was demonstrated both in mammary adenomas and adenocarcinomas in bitches (Figures 1A, 1B). In all the cases with positive reaction (90% adenocarcinomas and in 86% adenomas) a nuclear-cytoplasmic HIF-1α expression was noted. Moreover, in the two groups of tumours evident differences were seen in intensity of the protein expression. In adenocarcinomas, over 39% tumours manifested HIF-1α expression evaluated at +, over 26% expression evaluated at ++ and 25% at +++. In adenomas, 86% of the examined tumours manifested expression of the protein which, however, did not exceed the +intensity. It should be noted that over 9% of adenocarcinomas and over 13% of adenomas manifested no HIF-1α expression. Statistical analysis using Mann-Whitney test demonstrated a significantly higher HIF-1α expression in adenocarcinomas than in adenomas (P = 0.0004) (Figure 2A).


Expression of hypoxia-inducible factor-1α and vascular density in mammary adenomas and adenocarcinomas in bitches.

Madej JA, Madej JP, Dziegiel P, Pula B, Nowak M - Acta Vet. Scand. (2013)

Hypoxia-inducible factor-1α (HIF-1α) expression in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001; ****P < 0.0001, Mann–Whitney test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016321&req=5

Figure 2: Hypoxia-inducible factor-1α (HIF-1α) expression in adenomas and adenocarcinomas (A) and in relation to the grade of malignancy of the studied adenocarcinomas (B). ***P < 0.001; ****P < 0.0001, Mann–Whitney test.
Mentions: Expression of HIF-1α protein was demonstrated both in mammary adenomas and adenocarcinomas in bitches (Figures 1A, 1B). In all the cases with positive reaction (90% adenocarcinomas and in 86% adenomas) a nuclear-cytoplasmic HIF-1α expression was noted. Moreover, in the two groups of tumours evident differences were seen in intensity of the protein expression. In adenocarcinomas, over 39% tumours manifested HIF-1α expression evaluated at +, over 26% expression evaluated at ++ and 25% at +++. In adenomas, 86% of the examined tumours manifested expression of the protein which, however, did not exceed the +intensity. It should be noted that over 9% of adenocarcinomas and over 13% of adenomas manifested no HIF-1α expression. Statistical analysis using Mann-Whitney test demonstrated a significantly higher HIF-1α expression in adenocarcinomas than in adenomas (P = 0.0004) (Figure 2A).

Bottom Line: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation.A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004).Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland. marcin.nowak@up.wroc.pl.

ABSTRACT

Background: The study aimed at examining hypoxia-inducible factor (HIF)1α expression in adenocarcinomas and adenomas in bitches in regard to tumour malignancy grade, proliferation, apoptosis and vascularisation. Therefore, paraffin sections of 15 adenomas and 64 adenocarcinomas sampled from 79 dogs aged 6 to 16 years were analysed.

Results: A significantly higher HIF-1α expression was noted in adenocarcinomas in comparison to adenomas (P < 0.0004). Moreover, HIF-1α expression in adenocarcinomas correlated positively with tumour malignancy grade (r = 0.59, P < 0.05), Ki-67 antigen expression (r = 0.43; P < 0.0005), TUNEL-positive cells (r = 0.62, P < 0001) and tumour vascularity measured by quantification of vessels characterized by the expression of von Willebrand Factor (r = 0.57, P < 0.05).

Conclusion: Results of this study indicate a similar biological role of HIF-1α in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.

Show MeSH
Related in: MedlinePlus