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Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

Li J, Zhu L, Lu G, Zhan XB, Lin CC, Zheng ZY - PLoS ONE (2014)

Bottom Line: In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv.The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves.Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.

ABSTRACT
Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

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Potato yield under various treatments.Potatoes were harvested and weighed after 3-month cultivation. P. infestans infected plants were treated by 105 mL−1 of Phytophthora infestans zoospores at 45-day of cultivation. 1 d+P.i., 250 µg/mL curdlan oligosaccharides (CurdOs, the mixture of glucopentaose, glucohexaose, and glucoseptaose) was sprayed on the potato leaves 1 d before P. infestans treatment. 5 d+Pi, 250 µg/mL CurdOs was used 5 d before P. infestans treatment. CK, control without treatment. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).
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pone-0097197-g008: Potato yield under various treatments.Potatoes were harvested and weighed after 3-month cultivation. P. infestans infected plants were treated by 105 mL−1 of Phytophthora infestans zoospores at 45-day of cultivation. 1 d+P.i., 250 µg/mL curdlan oligosaccharides (CurdOs, the mixture of glucopentaose, glucohexaose, and glucoseptaose) was sprayed on the potato leaves 1 d before P. infestans treatment. 5 d+Pi, 250 µg/mL CurdOs was used 5 d before P. infestans treatment. CK, control without treatment. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).

Mentions: The potato yield after 3-month of cultivation was determined (Fig. 8). When treated with P. infestans, the whole plant yield of potato after harvest reached 6.82±2.80 g. Significant increase of potato yield was observed when the CurdOs were applied 1 d prior to P. infestans treatment (10.37±3.75 g, t′ = 2.741, t0.05(22) = 2.074, p = 0.012). However, the potato yield did not show significant difference from the P. infestans set when the plants were treated with CurdOs 5 d before the infection of the pathogen (p = 0.146). In comparison with the control set (CK), the potato plant yield in 1 d+P. i. set kept at the same level, which indicates that the CurdOs treatment shows protection effect on the plants. In addition, the average potato weight was not affected by the application of CurdOs. The results support the conclusion that CurdOs activate the potato defense responses against P. infestans infection for a short period of time.


Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

Li J, Zhu L, Lu G, Zhan XB, Lin CC, Zheng ZY - PLoS ONE (2014)

Potato yield under various treatments.Potatoes were harvested and weighed after 3-month cultivation. P. infestans infected plants were treated by 105 mL−1 of Phytophthora infestans zoospores at 45-day of cultivation. 1 d+P.i., 250 µg/mL curdlan oligosaccharides (CurdOs, the mixture of glucopentaose, glucohexaose, and glucoseptaose) was sprayed on the potato leaves 1 d before P. infestans treatment. 5 d+Pi, 250 µg/mL CurdOs was used 5 d before P. infestans treatment. CK, control without treatment. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016274&req=5

pone-0097197-g008: Potato yield under various treatments.Potatoes were harvested and weighed after 3-month cultivation. P. infestans infected plants were treated by 105 mL−1 of Phytophthora infestans zoospores at 45-day of cultivation. 1 d+P.i., 250 µg/mL curdlan oligosaccharides (CurdOs, the mixture of glucopentaose, glucohexaose, and glucoseptaose) was sprayed on the potato leaves 1 d before P. infestans treatment. 5 d+Pi, 250 µg/mL CurdOs was used 5 d before P. infestans treatment. CK, control without treatment. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).
Mentions: The potato yield after 3-month of cultivation was determined (Fig. 8). When treated with P. infestans, the whole plant yield of potato after harvest reached 6.82±2.80 g. Significant increase of potato yield was observed when the CurdOs were applied 1 d prior to P. infestans treatment (10.37±3.75 g, t′ = 2.741, t0.05(22) = 2.074, p = 0.012). However, the potato yield did not show significant difference from the P. infestans set when the plants were treated with CurdOs 5 d before the infection of the pathogen (p = 0.146). In comparison with the control set (CK), the potato plant yield in 1 d+P. i. set kept at the same level, which indicates that the CurdOs treatment shows protection effect on the plants. In addition, the average potato weight was not affected by the application of CurdOs. The results support the conclusion that CurdOs activate the potato defense responses against P. infestans infection for a short period of time.

Bottom Line: In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv.The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves.Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.

ABSTRACT
Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

Show MeSH
Related in: MedlinePlus