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Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

Li J, Zhu L, Lu G, Zhan XB, Lin CC, Zheng ZY - PLoS ONE (2014)

Bottom Line: In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv.The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves.Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.

ABSTRACT
Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

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Related in: MedlinePlus

Comparison of protein spots from the elicitor-treated sets with the elicitor-free control.Proteins were extracted from the leaf cells at 12-free sample (blank control). DP, degree of polymerization.
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pone-0097197-g006: Comparison of protein spots from the elicitor-treated sets with the elicitor-free control.Proteins were extracted from the leaf cells at 12-free sample (blank control). DP, degree of polymerization.

Mentions: As shown in Figure 6, the spot intensity increased along with the increment of DP of the curdlan oligosaccharide. In addition, the spot intensity kept visually stable from DP 5 to DP 7. A quantitative analysis of the spots intensity (illustrated as Figure S1) confirmed the observation that glucopentaose (DP 5) was the shortest active elicitor for potato plant treatment.


Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

Li J, Zhu L, Lu G, Zhan XB, Lin CC, Zheng ZY - PLoS ONE (2014)

Comparison of protein spots from the elicitor-treated sets with the elicitor-free control.Proteins were extracted from the leaf cells at 12-free sample (blank control). DP, degree of polymerization.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016274&req=5

pone-0097197-g006: Comparison of protein spots from the elicitor-treated sets with the elicitor-free control.Proteins were extracted from the leaf cells at 12-free sample (blank control). DP, degree of polymerization.
Mentions: As shown in Figure 6, the spot intensity increased along with the increment of DP of the curdlan oligosaccharide. In addition, the spot intensity kept visually stable from DP 5 to DP 7. A quantitative analysis of the spots intensity (illustrated as Figure S1) confirmed the observation that glucopentaose (DP 5) was the shortest active elicitor for potato plant treatment.

Bottom Line: In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv.The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves.Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.

ABSTRACT
Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

Show MeSH
Related in: MedlinePlus