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Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

Li J, Zhu L, Lu G, Zhan XB, Lin CC, Zheng ZY - PLoS ONE (2014)

Bottom Line: In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv.The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves.Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.

ABSTRACT
Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

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Long-term defense activities of potato in response to elicitors.The potato leaves were treated with various curdlan oligosaccharides (degree of polymerization (DP) from 3 to DP 7). Sampling was done at 20 d after elicitation for each set. Potato leaves treated with deionized water were used as elicitor-free control, CK. All tests were carried out in triplicates. PAL, phenylalanine ammonia-lyase; GLU, β-1,3-glucanase. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).
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pone-0097197-g003: Long-term defense activities of potato in response to elicitors.The potato leaves were treated with various curdlan oligosaccharides (degree of polymerization (DP) from 3 to DP 7). Sampling was done at 20 d after elicitation for each set. Potato leaves treated with deionized water were used as elicitor-free control, CK. All tests were carried out in triplicates. PAL, phenylalanine ammonia-lyase; GLU, β-1,3-glucanase. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).

Mentions: To determine the changes of the defense factors, potato plants were separately treated with various curdlan oligosaccharides (from DP 3 to DP 7). Accumulations of H2O2 and SA and the enzymatic activity of GLU were detected in potato leaves (Fig. 2). Within minutes, the early-defense response was observed, as the concentration of H2O2 increased considerably 5 min after elicitation and reached the maximum level at 10 min. The peak values of SA and GLU were both observed at 4 h. The response time for SA and GLU was much longer than H2O2, which indicated that the late-defense response was occurring in the cells. (Fig. 2, B and C). Additionally, the levels of all the three factors dropped gradually when the elicitation time prolonged which indicated that the CurdOs effect only lasted a short time in the leaf cells. Therefore, the H2O2 concentration, GLU and PAL activities were determined at 20 d after elicitation (Fig. 3). No significant difference was observed between the CurdOs treated plants and blank control (F(6,14), H2O2 = 0.050, pH2O2 = 0.999; F(6,14), GLU = 0.230, pGLU = 0.960; F(6,14), PAL = 0.098, pPAL = 0.995), which confirmed the conclusion that CurdOs activated the short-term elicitation effect in potato leaf cells.


Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

Li J, Zhu L, Lu G, Zhan XB, Lin CC, Zheng ZY - PLoS ONE (2014)

Long-term defense activities of potato in response to elicitors.The potato leaves were treated with various curdlan oligosaccharides (degree of polymerization (DP) from 3 to DP 7). Sampling was done at 20 d after elicitation for each set. Potato leaves treated with deionized water were used as elicitor-free control, CK. All tests were carried out in triplicates. PAL, phenylalanine ammonia-lyase; GLU, β-1,3-glucanase. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016274&req=5

pone-0097197-g003: Long-term defense activities of potato in response to elicitors.The potato leaves were treated with various curdlan oligosaccharides (degree of polymerization (DP) from 3 to DP 7). Sampling was done at 20 d after elicitation for each set. Potato leaves treated with deionized water were used as elicitor-free control, CK. All tests were carried out in triplicates. PAL, phenylalanine ammonia-lyase; GLU, β-1,3-glucanase. Values are means±SD. Lowercase letters indicate significant differences (LSD test at p<0.05).
Mentions: To determine the changes of the defense factors, potato plants were separately treated with various curdlan oligosaccharides (from DP 3 to DP 7). Accumulations of H2O2 and SA and the enzymatic activity of GLU were detected in potato leaves (Fig. 2). Within minutes, the early-defense response was observed, as the concentration of H2O2 increased considerably 5 min after elicitation and reached the maximum level at 10 min. The peak values of SA and GLU were both observed at 4 h. The response time for SA and GLU was much longer than H2O2, which indicated that the late-defense response was occurring in the cells. (Fig. 2, B and C). Additionally, the levels of all the three factors dropped gradually when the elicitation time prolonged which indicated that the CurdOs effect only lasted a short time in the leaf cells. Therefore, the H2O2 concentration, GLU and PAL activities were determined at 20 d after elicitation (Fig. 3). No significant difference was observed between the CurdOs treated plants and blank control (F(6,14), H2O2 = 0.050, pH2O2 = 0.999; F(6,14), GLU = 0.230, pGLU = 0.960; F(6,14), PAL = 0.098, pPAL = 0.995), which confirmed the conclusion that CurdOs activated the short-term elicitation effect in potato leaf cells.

Bottom Line: In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv.The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves.Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.

ABSTRACT
Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H₂O₂ and salicylic acid and the activities of phenylalanine amino-lyase, β-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually.

Show MeSH
Related in: MedlinePlus