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High expression of prolactin receptor is associated with cell survival in cervical cancer cells.

Lopez-Pulido EI, Muñoz-Valle JF, Del Toro-Arreola S, Jave-Suárez LF, Bueno-Topete MR, Estrada-Chávez C, Pereira-Suárez AL - Cancer Cell Int. (2013)

Bottom Line: The aim of this study was to evaluate the expression of PRL/PRLR and the effect of PRL treatment on cell proliferation and apoptosis in cervical cancer cell lines.Treatment with PRL (200 ng/ml) increased cell proliferation in HeLa cells determined by the MTT assay at day 3 and after 1 day a protective effect against etoposide induced apoptosis in HeLa, SiHa and C-33A cervical cancer cell lines analyzed by the TUNEL assay.The use of an effective PRL antagonist may provide a better therapeutic intervention in cervical cancer.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratorio de Inmunología, Departamento de Fisiología, Centro Universitario de, Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México. analauraps@hotmail.com.

ABSTRACT

Background: The altered expression of prolactin (PRL) and its receptor (PRLR) has been implicated in breast and other types of cancer. There are few studies that have focused on the analysis of PRL/PRLR in cervical cancer where the development of neoplastic lesions is influenced by the variation of the hormonal status. The aim of this study was to evaluate the expression of PRL/PRLR and the effect of PRL treatment on cell proliferation and apoptosis in cervical cancer cell lines.

Results: High expression of multiple PRLR forms and PRLvariants of 60-80 kDa were observed in cervical cancer cell lines compared with non-tumorigenic keratinocytes evaluated by Western blot, immunofluorecence and real time PCR. Treatment with PRL (200 ng/ml) increased cell proliferation in HeLa cells determined by the MTT assay at day 3 and after 1 day a protective effect against etoposide induced apoptosis in HeLa, SiHa and C-33A cervical cancer cell lines analyzed by the TUNEL assay.

Conclusions: Our data suggests that PRL/PRLR signaling could act as an important survival factor for cervical cancer. The use of an effective PRL antagonist may provide a better therapeutic intervention in cervical cancer.

No MeSH data available.


Related in: MedlinePlus

Effects of PRL and PRL or PRLR blocking antibodies on proliferation of cervical cancer cells. Effects on metabolic activity after the incubation with PRL (200 ng/ml), PRL-AB (200 ng/ml) or PRLR-AB (2.5 μg) for 3 or 5 days in HeLa, SiHa, C-33A (A, B, C) and control cells MCF-7, T-47D and HaCaT (D, E, F). Graphs show experiments performed in triplicate, which are repeated at least three times. *p<.05.
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Figure 3: Effects of PRL and PRL or PRLR blocking antibodies on proliferation of cervical cancer cells. Effects on metabolic activity after the incubation with PRL (200 ng/ml), PRL-AB (200 ng/ml) or PRLR-AB (2.5 μg) for 3 or 5 days in HeLa, SiHa, C-33A (A, B, C) and control cells MCF-7, T-47D and HaCaT (D, E, F). Graphs show experiments performed in triplicate, which are repeated at least three times. *p<.05.

Mentions: To test whether PRL had an effect on the proliferation in cervical cancer cells, we cultured cells in the absence and presence of PRL, PRLR-AB or PRL-AB for 3 and 5 days. Treatment with PRL (200 ng/ml) increased the viable cell number in HeLa about 9% after 3 days of incubation (Figure 3A); while no effect was observed in SiHa and C-33A (Figure 3B, C). The incubation with PRLR-AB (2.5 μg) or PRL-AB (200 ng/ml) had not impact over the total cell number in cervical cancer cells. Similar dose response analyzes with PRL and blocking antibodies were performed in breast cancer and HaCat cells, observing an increase of 6% in the cell number of T47-D after 3 days of incubation with PRL. Moreover, the treatment with PRL and PRLR antibodies decreased the total cell number in a range of 5–8% in T47-D and 12-18% in MCF-7 at day 3 (Figure 3D, E). But, this decrease was statistically significant only in MCF-7 after 3 days of incubation with the PRL-AB. No effect on cell proliferation in HaCat cells was observed (Figure 3F). Other dose and time conditions were used in all the cell lines and no significant difference was detected in the total cell number (data not shown).


High expression of prolactin receptor is associated with cell survival in cervical cancer cells.

Lopez-Pulido EI, Muñoz-Valle JF, Del Toro-Arreola S, Jave-Suárez LF, Bueno-Topete MR, Estrada-Chávez C, Pereira-Suárez AL - Cancer Cell Int. (2013)

Effects of PRL and PRL or PRLR blocking antibodies on proliferation of cervical cancer cells. Effects on metabolic activity after the incubation with PRL (200 ng/ml), PRL-AB (200 ng/ml) or PRLR-AB (2.5 μg) for 3 or 5 days in HeLa, SiHa, C-33A (A, B, C) and control cells MCF-7, T-47D and HaCaT (D, E, F). Graphs show experiments performed in triplicate, which are repeated at least three times. *p<.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016267&req=5

Figure 3: Effects of PRL and PRL or PRLR blocking antibodies on proliferation of cervical cancer cells. Effects on metabolic activity after the incubation with PRL (200 ng/ml), PRL-AB (200 ng/ml) or PRLR-AB (2.5 μg) for 3 or 5 days in HeLa, SiHa, C-33A (A, B, C) and control cells MCF-7, T-47D and HaCaT (D, E, F). Graphs show experiments performed in triplicate, which are repeated at least three times. *p<.05.
Mentions: To test whether PRL had an effect on the proliferation in cervical cancer cells, we cultured cells in the absence and presence of PRL, PRLR-AB or PRL-AB for 3 and 5 days. Treatment with PRL (200 ng/ml) increased the viable cell number in HeLa about 9% after 3 days of incubation (Figure 3A); while no effect was observed in SiHa and C-33A (Figure 3B, C). The incubation with PRLR-AB (2.5 μg) or PRL-AB (200 ng/ml) had not impact over the total cell number in cervical cancer cells. Similar dose response analyzes with PRL and blocking antibodies were performed in breast cancer and HaCat cells, observing an increase of 6% in the cell number of T47-D after 3 days of incubation with PRL. Moreover, the treatment with PRL and PRLR antibodies decreased the total cell number in a range of 5–8% in T47-D and 12-18% in MCF-7 at day 3 (Figure 3D, E). But, this decrease was statistically significant only in MCF-7 after 3 days of incubation with the PRL-AB. No effect on cell proliferation in HaCat cells was observed (Figure 3F). Other dose and time conditions were used in all the cell lines and no significant difference was detected in the total cell number (data not shown).

Bottom Line: The aim of this study was to evaluate the expression of PRL/PRLR and the effect of PRL treatment on cell proliferation and apoptosis in cervical cancer cell lines.Treatment with PRL (200 ng/ml) increased cell proliferation in HeLa cells determined by the MTT assay at day 3 and after 1 day a protective effect against etoposide induced apoptosis in HeLa, SiHa and C-33A cervical cancer cell lines analyzed by the TUNEL assay.The use of an effective PRL antagonist may provide a better therapeutic intervention in cervical cancer.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratorio de Inmunología, Departamento de Fisiología, Centro Universitario de, Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México. analauraps@hotmail.com.

ABSTRACT

Background: The altered expression of prolactin (PRL) and its receptor (PRLR) has been implicated in breast and other types of cancer. There are few studies that have focused on the analysis of PRL/PRLR in cervical cancer where the development of neoplastic lesions is influenced by the variation of the hormonal status. The aim of this study was to evaluate the expression of PRL/PRLR and the effect of PRL treatment on cell proliferation and apoptosis in cervical cancer cell lines.

Results: High expression of multiple PRLR forms and PRLvariants of 60-80 kDa were observed in cervical cancer cell lines compared with non-tumorigenic keratinocytes evaluated by Western blot, immunofluorecence and real time PCR. Treatment with PRL (200 ng/ml) increased cell proliferation in HeLa cells determined by the MTT assay at day 3 and after 1 day a protective effect against etoposide induced apoptosis in HeLa, SiHa and C-33A cervical cancer cell lines analyzed by the TUNEL assay.

Conclusions: Our data suggests that PRL/PRLR signaling could act as an important survival factor for cervical cancer. The use of an effective PRL antagonist may provide a better therapeutic intervention in cervical cancer.

No MeSH data available.


Related in: MedlinePlus