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Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.

Yao H, Nabika T - PLoS ONE (2014)

Bottom Line: Salt loading did not affect blood pressure levels in SHR and WKY.After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group.These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Neurochemistry, National Hospital Organization Hizen Psychiatric Center, Saga, Japan.

ABSTRACT
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

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Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in WKY/Izm.Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14 in Wistar-Kyoto rats/Izumo strain (WKY/Izm)(A). Infarct volume after distal middle cerebral artery occlusion (MCAO) was not significantly different between the salt-loaded and the control groups. Dada are presented as mean±S.D. (n = 5–6) (B).
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pone-0097109-g005: Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in WKY/Izm.Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14 in Wistar-Kyoto rats/Izumo strain (WKY/Izm)(A). Infarct volume after distal middle cerebral artery occlusion (MCAO) was not significantly different between the salt-loaded and the control groups. Dada are presented as mean±S.D. (n = 5–6) (B).

Mentions: Two-way analysis of variance revealed a significant effect of saline on the volumes of drinking (p = 0.004), but not significant for Day 1, 7 and 14 (p = 0.159) (Figure 5A). Resting MABP was 98±16 mmHg in the salt loaded group, which was not significantly different from 100±14 mmHg in the control groups. Body weight, head and rectal temperatures, blood gases, and glucose were within the normal range, and were not different between the groups (data not shown). Infarct volumes were not different between the salt-loaded and control groups (45±19 mm3 vs. 38±9 mm3, respectively) (Figure 5B).


Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.

Yao H, Nabika T - PLoS ONE (2014)

Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in WKY/Izm.Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14 in Wistar-Kyoto rats/Izumo strain (WKY/Izm)(A). Infarct volume after distal middle cerebral artery occlusion (MCAO) was not significantly different between the salt-loaded and the control groups. Dada are presented as mean±S.D. (n = 5–6) (B).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016244&req=5

pone-0097109-g005: Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in WKY/Izm.Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14 in Wistar-Kyoto rats/Izumo strain (WKY/Izm)(A). Infarct volume after distal middle cerebral artery occlusion (MCAO) was not significantly different between the salt-loaded and the control groups. Dada are presented as mean±S.D. (n = 5–6) (B).
Mentions: Two-way analysis of variance revealed a significant effect of saline on the volumes of drinking (p = 0.004), but not significant for Day 1, 7 and 14 (p = 0.159) (Figure 5A). Resting MABP was 98±16 mmHg in the salt loaded group, which was not significantly different from 100±14 mmHg in the control groups. Body weight, head and rectal temperatures, blood gases, and glucose were within the normal range, and were not different between the groups (data not shown). Infarct volumes were not different between the salt-loaded and control groups (45±19 mm3 vs. 38±9 mm3, respectively) (Figure 5B).

Bottom Line: Salt loading did not affect blood pressure levels in SHR and WKY.After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group.These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Neurochemistry, National Hospital Organization Hizen Psychiatric Center, Saga, Japan.

ABSTRACT
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

Show MeSH
Related in: MedlinePlus