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Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.

Yao H, Nabika T - PLoS ONE (2014)

Bottom Line: Salt loading did not affect blood pressure levels in SHR and WKY.After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group.These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Neurochemistry, National Hospital Organization Hizen Psychiatric Center, Saga, Japan.

ABSTRACT
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

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Related in: MedlinePlus

Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in SHR/Izm.Representative brain sections stained with 2,3,5-triphenyltetrazolium chloride from spontaneously hypertensive rats/Izumo strain (SHR/Izm) subjected to distal middle cerebral artery occlusion (MCAO) 48 h earlier (A). Average-sized cortical infarcts in rats with or without salt loading are presented. Infarct volume after distal MCAO in the salt-loaded group was significantly increased compared to the control group (*p = 0.002, n = 9–13) (B). Bars represent mean values.
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pone-0097109-g003: Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in SHR/Izm.Representative brain sections stained with 2,3,5-triphenyltetrazolium chloride from spontaneously hypertensive rats/Izumo strain (SHR/Izm) subjected to distal middle cerebral artery occlusion (MCAO) 48 h earlier (A). Average-sized cortical infarcts in rats with or without salt loading are presented. Infarct volume after distal MCAO in the salt-loaded group was significantly increased compared to the control group (*p = 0.002, n = 9–13) (B). Bars represent mean values.

Mentions: Two salt-loaded and 1 control SHR/Izm died during the 48 h of survival period after MCAO. Infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002) (Figure 3B).


Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.

Yao H, Nabika T - PLoS ONE (2014)

Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in SHR/Izm.Representative brain sections stained with 2,3,5-triphenyltetrazolium chloride from spontaneously hypertensive rats/Izumo strain (SHR/Izm) subjected to distal middle cerebral artery occlusion (MCAO) 48 h earlier (A). Average-sized cortical infarcts in rats with or without salt loading are presented. Infarct volume after distal MCAO in the salt-loaded group was significantly increased compared to the control group (*p = 0.002, n = 9–13) (B). Bars represent mean values.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016244&req=5

pone-0097109-g003: Infarct volume after distal middle cerebral artery occlusion (MCAO) in the salt-loaded and the control groups in SHR/Izm.Representative brain sections stained with 2,3,5-triphenyltetrazolium chloride from spontaneously hypertensive rats/Izumo strain (SHR/Izm) subjected to distal middle cerebral artery occlusion (MCAO) 48 h earlier (A). Average-sized cortical infarcts in rats with or without salt loading are presented. Infarct volume after distal MCAO in the salt-loaded group was significantly increased compared to the control group (*p = 0.002, n = 9–13) (B). Bars represent mean values.
Mentions: Two salt-loaded and 1 control SHR/Izm died during the 48 h of survival period after MCAO. Infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002) (Figure 3B).

Bottom Line: Salt loading did not affect blood pressure levels in SHR and WKY.After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group.These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Neurochemistry, National Hospital Organization Hizen Psychiatric Center, Saga, Japan.

ABSTRACT
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

Show MeSH
Related in: MedlinePlus