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Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.

Yao H, Nabika T - PLoS ONE (2014)

Bottom Line: Salt loading did not affect blood pressure levels in SHR and WKY.After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group.These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Neurochemistry, National Hospital Organization Hizen Psychiatric Center, Saga, Japan.

ABSTRACT
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

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Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14.The volume of water intake was stable during the experimental period, while the volume of saline intake progressively increased from Day 1 to Day 14. Values are mean±S.D. (n = 8–9).
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pone-0097109-g002: Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14.The volume of water intake was stable during the experimental period, while the volume of saline intake progressively increased from Day 1 to Day 14. Values are mean±S.D. (n = 8–9).

Mentions: The volumes of drinking water during 24-hour period were approximately 30 mL in the control group, while SHR showed an exaggerated preference for NaCl (n = 8–9) (Figure 2). Two-way analysis of variance revealed a significant effect of saline on the volumes of drinking (p<0.001), and a strong tendency to an increased intake of saline from Day 1 to Day 14 (p = 0.052).


Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.

Yao H, Nabika T - PLoS ONE (2014)

Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14.The volume of water intake was stable during the experimental period, while the volume of saline intake progressively increased from Day 1 to Day 14. Values are mean±S.D. (n = 8–9).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016244&req=5

pone-0097109-g002: Amount of drinking of water or saline (0.9% NaCl) determined at Day 1, Day 7, and Day 14.The volume of water intake was stable during the experimental period, while the volume of saline intake progressively increased from Day 1 to Day 14. Values are mean±S.D. (n = 8–9).
Mentions: The volumes of drinking water during 24-hour period were approximately 30 mL in the control group, while SHR showed an exaggerated preference for NaCl (n = 8–9) (Figure 2). Two-way analysis of variance revealed a significant effect of saline on the volumes of drinking (p<0.001), and a strong tendency to an increased intake of saline from Day 1 to Day 14 (p = 0.052).

Bottom Line: Salt loading did not affect blood pressure levels in SHR and WKY.After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group.These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Neurochemistry, National Hospital Organization Hizen Psychiatric Center, Saga, Japan.

ABSTRACT
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

Show MeSH
Related in: MedlinePlus