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Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea.

Hong HC, Li SL, Zhang XQ, Ye WC, Zhang QW - Chin Med (2013)

Bottom Line: The four flavonoids (1-4) showed α-glucosidase inhibitory activities, with rutin (1) and astragalin (4) showing high α-glucosidase inhibitory activities (IC50 values of 13.19 ± 1.10 and 15.82 ± 1.11 μM, respectively).The total contents of the four flavonoids were different among eight samples examined, ranging from 4.34 mg/g to 0.53 mg/g.The four flavonoids in M. atropurpurea leaves could inhibit α-glucosidase activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. qwzhang@umac.mo.

ABSTRACT

Background: This study aims to isolate the α-glucosidase inhibitory compounds from mulberry leaves (Morus atropurpurea Roxb., Moraceae) and to develop an analytical method for quantification of the compounds.

Methods: Four flavonoids, rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3) and astragalin (4), were isolated by column chromatography from mulberry leaf water extracts (MWE). The α-glucosidase inhibitory activities of MWE and the four isolated compounds were evaluated by a microplate-based in vitro assay. The content of the isolated flavonoids in M. atropurpurea leaves purchased from different local herbal stores or collected in different locations was determined by high performance liquid chromatography.

Results: The four flavonoids (1-4) showed α-glucosidase inhibitory activities, with rutin (1) and astragalin (4) showing high α-glucosidase inhibitory activities (IC50 values of 13.19 ± 1.10 and 15.82 ± 1.11 μM, respectively). The total contents of the four flavonoids were different among eight samples examined, ranging from 4.34 mg/g to 0.53 mg/g.

Conclusions: The four flavonoids in M. atropurpurea leaves could inhibit α-glucosidase activity.

No MeSH data available.


Structures of the four flavonoids (1–4) extracted from M. atropurpurea.
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Figure 1: Structures of the four flavonoids (1–4) extracted from M. atropurpurea.

Mentions: Four flavonoids isolated from the M. atropurpurea leaves were identified as rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3), and astragalin (4) (shown in Figure 1) by comparing the MS and NMR data with the literature[10]. The 1H NMR spectra revealed that their purities were all above 98%, which was also confirmed by HPLC.


Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea.

Hong HC, Li SL, Zhang XQ, Ye WC, Zhang QW - Chin Med (2013)

Structures of the four flavonoids (1–4) extracted from M. atropurpurea.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016240&req=5

Figure 1: Structures of the four flavonoids (1–4) extracted from M. atropurpurea.
Mentions: Four flavonoids isolated from the M. atropurpurea leaves were identified as rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3), and astragalin (4) (shown in Figure 1) by comparing the MS and NMR data with the literature[10]. The 1H NMR spectra revealed that their purities were all above 98%, which was also confirmed by HPLC.

Bottom Line: The four flavonoids (1-4) showed α-glucosidase inhibitory activities, with rutin (1) and astragalin (4) showing high α-glucosidase inhibitory activities (IC50 values of 13.19 ± 1.10 and 15.82 ± 1.11 μM, respectively).The total contents of the four flavonoids were different among eight samples examined, ranging from 4.34 mg/g to 0.53 mg/g.The four flavonoids in M. atropurpurea leaves could inhibit α-glucosidase activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. qwzhang@umac.mo.

ABSTRACT

Background: This study aims to isolate the α-glucosidase inhibitory compounds from mulberry leaves (Morus atropurpurea Roxb., Moraceae) and to develop an analytical method for quantification of the compounds.

Methods: Four flavonoids, rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3) and astragalin (4), were isolated by column chromatography from mulberry leaf water extracts (MWE). The α-glucosidase inhibitory activities of MWE and the four isolated compounds were evaluated by a microplate-based in vitro assay. The content of the isolated flavonoids in M. atropurpurea leaves purchased from different local herbal stores or collected in different locations was determined by high performance liquid chromatography.

Results: The four flavonoids (1-4) showed α-glucosidase inhibitory activities, with rutin (1) and astragalin (4) showing high α-glucosidase inhibitory activities (IC50 values of 13.19 ± 1.10 and 15.82 ± 1.11 μM, respectively). The total contents of the four flavonoids were different among eight samples examined, ranging from 4.34 mg/g to 0.53 mg/g.

Conclusions: The four flavonoids in M. atropurpurea leaves could inhibit α-glucosidase activity.

No MeSH data available.