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Acute oral intake of a higenamine-based dietary supplement increases circulating free fatty acids and energy expenditure in human subjects.

Lee SR, Schriefer JM, Gunnels TA, Harvey IC, Bloomer RJ - Lipids Health Dis (2013)

Bottom Line: A condition effect was noted for both FFA (p < 0.0001) and kilocalorie expenditure (p = 0.001), with values higher for supplement compared to placebo at 60, 120, and 180 minutes post ingestion.No statistically significant effects were noted for glycerol or RER (p > 0.05).A condition effect was noted for heart rate (p = 0.03) and systolic blood pressure (p < 0.0001), with values higher for supplement compared to placebo.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Health and Sport Sciences, Cardiorespiratory/Metabolic Laboratory, University of Memphis, 106 Roane Field House, Memphis, TN 38152, USA. rbloomer@memphis.edu.

ABSTRACT

Background: Higenamine, also known as norcoclaurine, is an herbal constituent thought to act as a beta-2 adrenergic receptor agonist-possibly stimulating lipolysis. It was the purpose of this study to determine the impact of a higenamine-based dietary supplement on plasma free fatty acids and energy expenditure following acute oral ingestion.

Methods: Sixteen healthy subjects (8 men; 26.1 ± 2.5 yrs; 8 women 22.4 ± 3.1 yrs) ingested a dietary supplement containing a combination of higenamine, caffeine (270 mg), and yohimbe bark extract or a placebo, on two separate occasions in a double-blind, randomized, cross-over design, separated by 6-8 days. Blood samples were collected immediately before ingestion, and at 30, 60, 120, and 180 minutes post ingestion, and analyzed for plasma free fatty acids (FFA) and glycerol. Breath samples were collected at the same times for a measure of kilocalorie expenditure and respiratory exchange ratio (RER) using indirect calorimetry. Heart rate and blood pressure were recorded at all times. Data collection occurred in the morning following a 10 hour overnight fast.

Results: A condition effect was noted for both FFA (p < 0.0001) and kilocalorie expenditure (p = 0.001), with values higher for supplement compared to placebo at 60, 120, and 180 minutes post ingestion. No statistically significant effects were noted for glycerol or RER (p > 0.05). A condition effect was noted for heart rate (p = 0.03) and systolic blood pressure (p < 0.0001), with values higher for supplement compared to placebo.

Conclusion: Ingestion of a higenamine-based dietary supplement stimulates lipolysis and energy expenditure, as evidenced by a significant increase in circulating FFA and kilocalorie expenditure. The same supplement results in a moderate increase in heart rate (~3 bpm) and systolic blood pressure (~12 mmHg), which is consistent with previous studies evaluating moderate doses of caffeine and yohimbine, suggesting that higenamine contributes little to the increase in these hemodynamic variables. These findings are in reference to young, healthy and active men and women.

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Plasma free fatty acids (A) and glycerol (B) before and following ingestion of supplement or placebo. Data are mean ± SEM. *Condition effect noted for free fatty acids (p < 0.0001). **Time effect noted for free fatty acids (p = 0.0009); values higher at 60 min, 120 min, and 180 min compared to 30 min; values higher at 180 min compared to pre. †Difference noted at 60 min (p = 0.0004), 120 min (p = 0.0004), and 180 min (p = 0.004) between supplement and placebo. Interaction effect noted for free fatty acids (p = 0.05). No statistically significant effects noted for glycerol (p > 0.05).
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Figure 1: Plasma free fatty acids (A) and glycerol (B) before and following ingestion of supplement or placebo. Data are mean ± SEM. *Condition effect noted for free fatty acids (p < 0.0001). **Time effect noted for free fatty acids (p = 0.0009); values higher at 60 min, 120 min, and 180 min compared to 30 min; values higher at 180 min compared to pre. †Difference noted at 60 min (p = 0.0004), 120 min (p = 0.0004), and 180 min (p = 0.004) between supplement and placebo. Interaction effect noted for free fatty acids (p = 0.05). No statistically significant effects noted for glycerol (p > 0.05).

Mentions: Regarding FFA, a condition effect was noted (p < 0.0001), with values higher for the supplement compared to placebo. A time effect was also noted (p = 0.0009), with values higher at 60 minutes, 120 minutes, and 180 minutes compared to 30 minutes; values were also higher at 180 minutes compared to pre. An interaction effect was noted (p = 0.05). Contrasts revealed significant differences between supplement and placebo at 60 minutes (p = 0.0004), 120 minutes (p = 0.0004), and 180 minutes post ingestion (p = 0.004). Regarding glycerol, no condition (p = 0.20), time (p = 0.27), or interaction (p = 0.72) effects were noted. Data for FFA and glycerol are presented in Figure 1. Men and women responded in a similar manner to supplement and placebo with regards to FFA and glycerol.


Acute oral intake of a higenamine-based dietary supplement increases circulating free fatty acids and energy expenditure in human subjects.

Lee SR, Schriefer JM, Gunnels TA, Harvey IC, Bloomer RJ - Lipids Health Dis (2013)

Plasma free fatty acids (A) and glycerol (B) before and following ingestion of supplement or placebo. Data are mean ± SEM. *Condition effect noted for free fatty acids (p < 0.0001). **Time effect noted for free fatty acids (p = 0.0009); values higher at 60 min, 120 min, and 180 min compared to 30 min; values higher at 180 min compared to pre. †Difference noted at 60 min (p = 0.0004), 120 min (p = 0.0004), and 180 min (p = 0.004) between supplement and placebo. Interaction effect noted for free fatty acids (p = 0.05). No statistically significant effects noted for glycerol (p > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016229&req=5

Figure 1: Plasma free fatty acids (A) and glycerol (B) before and following ingestion of supplement or placebo. Data are mean ± SEM. *Condition effect noted for free fatty acids (p < 0.0001). **Time effect noted for free fatty acids (p = 0.0009); values higher at 60 min, 120 min, and 180 min compared to 30 min; values higher at 180 min compared to pre. †Difference noted at 60 min (p = 0.0004), 120 min (p = 0.0004), and 180 min (p = 0.004) between supplement and placebo. Interaction effect noted for free fatty acids (p = 0.05). No statistically significant effects noted for glycerol (p > 0.05).
Mentions: Regarding FFA, a condition effect was noted (p < 0.0001), with values higher for the supplement compared to placebo. A time effect was also noted (p = 0.0009), with values higher at 60 minutes, 120 minutes, and 180 minutes compared to 30 minutes; values were also higher at 180 minutes compared to pre. An interaction effect was noted (p = 0.05). Contrasts revealed significant differences between supplement and placebo at 60 minutes (p = 0.0004), 120 minutes (p = 0.0004), and 180 minutes post ingestion (p = 0.004). Regarding glycerol, no condition (p = 0.20), time (p = 0.27), or interaction (p = 0.72) effects were noted. Data for FFA and glycerol are presented in Figure 1. Men and women responded in a similar manner to supplement and placebo with regards to FFA and glycerol.

Bottom Line: A condition effect was noted for both FFA (p < 0.0001) and kilocalorie expenditure (p = 0.001), with values higher for supplement compared to placebo at 60, 120, and 180 minutes post ingestion.No statistically significant effects were noted for glycerol or RER (p > 0.05).A condition effect was noted for heart rate (p = 0.03) and systolic blood pressure (p < 0.0001), with values higher for supplement compared to placebo.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Health and Sport Sciences, Cardiorespiratory/Metabolic Laboratory, University of Memphis, 106 Roane Field House, Memphis, TN 38152, USA. rbloomer@memphis.edu.

ABSTRACT

Background: Higenamine, also known as norcoclaurine, is an herbal constituent thought to act as a beta-2 adrenergic receptor agonist-possibly stimulating lipolysis. It was the purpose of this study to determine the impact of a higenamine-based dietary supplement on plasma free fatty acids and energy expenditure following acute oral ingestion.

Methods: Sixteen healthy subjects (8 men; 26.1 ± 2.5 yrs; 8 women 22.4 ± 3.1 yrs) ingested a dietary supplement containing a combination of higenamine, caffeine (270 mg), and yohimbe bark extract or a placebo, on two separate occasions in a double-blind, randomized, cross-over design, separated by 6-8 days. Blood samples were collected immediately before ingestion, and at 30, 60, 120, and 180 minutes post ingestion, and analyzed for plasma free fatty acids (FFA) and glycerol. Breath samples were collected at the same times for a measure of kilocalorie expenditure and respiratory exchange ratio (RER) using indirect calorimetry. Heart rate and blood pressure were recorded at all times. Data collection occurred in the morning following a 10 hour overnight fast.

Results: A condition effect was noted for both FFA (p < 0.0001) and kilocalorie expenditure (p = 0.001), with values higher for supplement compared to placebo at 60, 120, and 180 minutes post ingestion. No statistically significant effects were noted for glycerol or RER (p > 0.05). A condition effect was noted for heart rate (p = 0.03) and systolic blood pressure (p < 0.0001), with values higher for supplement compared to placebo.

Conclusion: Ingestion of a higenamine-based dietary supplement stimulates lipolysis and energy expenditure, as evidenced by a significant increase in circulating FFA and kilocalorie expenditure. The same supplement results in a moderate increase in heart rate (~3 bpm) and systolic blood pressure (~12 mmHg), which is consistent with previous studies evaluating moderate doses of caffeine and yohimbine, suggesting that higenamine contributes little to the increase in these hemodynamic variables. These findings are in reference to young, healthy and active men and women.

Show MeSH
Related in: MedlinePlus