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Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

Arshad W, Haq IU, Waheed MT, Mysore KS, Mirza B - PLoS ONE (2014)

Bottom Line: Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum.Contrarily, transformed plants exhibited altered morphology and reduced fruit yield.In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT
Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

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Biochemical analysis of rolB transformed tomato plants.(A) Determination of Lycopene content (B) Ascorbic acid content (C) Total phenolic content and (D) Antioxidant activity along with their relative increase in fruits of rolB transgenic lines of tomato. RB  =  rolB transformed; GUS =  GUS transformed control; WT  =  Wild-type (untransformed control). Data represents mean of three replicates. Any two values with same alphabet did not differ significantly at 5% probability level using LSD test.
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pone-0096979-g003: Biochemical analysis of rolB transformed tomato plants.(A) Determination of Lycopene content (B) Ascorbic acid content (C) Total phenolic content and (D) Antioxidant activity along with their relative increase in fruits of rolB transgenic lines of tomato. RB  =  rolB transformed; GUS =  GUS transformed control; WT  =  Wild-type (untransformed control). Data represents mean of three replicates. Any two values with same alphabet did not differ significantly at 5% probability level using LSD test.

Mentions: To investigate the effect of rolB expression on important nutritional contents, biochemical analyses were performed on the fruits generated from rolB expressing plants. Strikingly, all the rolB expressing tomato lines showed significant increase (18 to 62%) in their lycopene content when compared to control and WT. The line RB I exhibited maximum increase of 62% in lycopene content (76 µg/g) while two other lines RB VII and RB IX showed an increase of 59% (74 µg/g) and 58% (74 µg/g), respectively in their lycopene content when compared to controls (Figure 3A). All the tested rolB expressing tomato fruits showed significantly higher (24 to 225%) ascorbic acid content than the controls. The highest relative increase (225%) in ascorbic acid level was observed for RB I with 333 µg/g ascorbic acid (Figure 3B). In addition, rolB expressing tomato fruits exhibited 11% to 58% increase in total phenolic content. Two rolB lines RB IX and RB VII exhibited maximum increase of 58% (21 mg/g) and 53% (20 mg/g), respectively, in their phenolic contents (Figure 3C). Considerable increase in the antioxidant activities was also observed for all rolB transformed plants as compared to controls. Highest relative increase (26%) in antioxidant activity was exhibited by RB I (EC50 = 536 µg/mL) followed by RB VII (EC50 = 558 µg/mL) and RB IX (EC50 = 564 µg/mL) which showed 23% and 22% increase in their antioxidant capacities, respectively (Figure 3D).


Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

Arshad W, Haq IU, Waheed MT, Mysore KS, Mirza B - PLoS ONE (2014)

Biochemical analysis of rolB transformed tomato plants.(A) Determination of Lycopene content (B) Ascorbic acid content (C) Total phenolic content and (D) Antioxidant activity along with their relative increase in fruits of rolB transgenic lines of tomato. RB  =  rolB transformed; GUS =  GUS transformed control; WT  =  Wild-type (untransformed control). Data represents mean of three replicates. Any two values with same alphabet did not differ significantly at 5% probability level using LSD test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016209&req=5

pone-0096979-g003: Biochemical analysis of rolB transformed tomato plants.(A) Determination of Lycopene content (B) Ascorbic acid content (C) Total phenolic content and (D) Antioxidant activity along with their relative increase in fruits of rolB transgenic lines of tomato. RB  =  rolB transformed; GUS =  GUS transformed control; WT  =  Wild-type (untransformed control). Data represents mean of three replicates. Any two values with same alphabet did not differ significantly at 5% probability level using LSD test.
Mentions: To investigate the effect of rolB expression on important nutritional contents, biochemical analyses were performed on the fruits generated from rolB expressing plants. Strikingly, all the rolB expressing tomato lines showed significant increase (18 to 62%) in their lycopene content when compared to control and WT. The line RB I exhibited maximum increase of 62% in lycopene content (76 µg/g) while two other lines RB VII and RB IX showed an increase of 59% (74 µg/g) and 58% (74 µg/g), respectively in their lycopene content when compared to controls (Figure 3A). All the tested rolB expressing tomato fruits showed significantly higher (24 to 225%) ascorbic acid content than the controls. The highest relative increase (225%) in ascorbic acid level was observed for RB I with 333 µg/g ascorbic acid (Figure 3B). In addition, rolB expressing tomato fruits exhibited 11% to 58% increase in total phenolic content. Two rolB lines RB IX and RB VII exhibited maximum increase of 58% (21 mg/g) and 53% (20 mg/g), respectively, in their phenolic contents (Figure 3C). Considerable increase in the antioxidant activities was also observed for all rolB transformed plants as compared to controls. Highest relative increase (26%) in antioxidant activity was exhibited by RB I (EC50 = 536 µg/mL) followed by RB VII (EC50 = 558 µg/mL) and RB IX (EC50 = 564 µg/mL) which showed 23% and 22% increase in their antioxidant capacities, respectively (Figure 3D).

Bottom Line: Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum.Contrarily, transformed plants exhibited altered morphology and reduced fruit yield.In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT
Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

Show MeSH
Related in: MedlinePlus