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Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

Arshad W, Haq IU, Waheed MT, Mysore KS, Mirza B - PLoS ONE (2014)

Bottom Line: Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum.Contrarily, transformed plants exhibited altered morphology and reduced fruit yield.In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT
Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

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Morphological analysis of rolB transgenic tomato plants cv. Rio Grande.(a) mature plants (b) leaves (c) roots (d) tomato fruit (whole and TS).
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pone-0096979-g002: Morphological analysis of rolB transgenic tomato plants cv. Rio Grande.(a) mature plants (b) leaves (c) roots (d) tomato fruit (whole and TS).

Mentions: The rolB expressing transgenic plants had a change in their vegetative growth and morphology when compared to WT tomato plants. However, no significant difference was observed between transformed control (GUS transformed plants) and WT tomato plants. All rolB transgenic lines were significantly shorter and produced smaller fruits when compared to WT plants (Figure 2a). The rolB expressing tomato plants had an average height of 42.3 cm as compared to 94 cm for WT plants. A reduction in apical dominance along with shorter internodal length was also observed in rolB expressing plants. Leaves were also smaller, less serrated and more oval in shape as compared to GUS control and WT. In some rolB transformed plants, mild wrinkled leaves were observed (Figure 2c). Interestingly, rolB expressing plants had profuse, long and hairy root system as compared to control (Figure 2d). The flowers were normal in size but some of them were infertile and did not produce fruits. Fruits that developed in rolB transgenic plants were smaller in size (Figure 2b) and matured earlier when compared to the fruits of control and WT plants. Number of fruits and their weight was calculated from 24 rolB expressing plants that were derived from nine independent transgenic lines. The rolB expressing tomato plants produced an average of 11 fruits per plant whereas control and WT plants produced and an average of 15 fruits per plant. Average fruit weight of rolB transgenic tomatoes was 38 g and was significantly lower than control/WT plants that had 74 g.


Agrobacterium-mediated transformation of tomato with rolB gene results in enhancement of fruit quality and foliar resistance against fungal pathogens.

Arshad W, Haq IU, Waheed MT, Mysore KS, Mirza B - PLoS ONE (2014)

Morphological analysis of rolB transgenic tomato plants cv. Rio Grande.(a) mature plants (b) leaves (c) roots (d) tomato fruit (whole and TS).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016209&req=5

pone-0096979-g002: Morphological analysis of rolB transgenic tomato plants cv. Rio Grande.(a) mature plants (b) leaves (c) roots (d) tomato fruit (whole and TS).
Mentions: The rolB expressing transgenic plants had a change in their vegetative growth and morphology when compared to WT tomato plants. However, no significant difference was observed between transformed control (GUS transformed plants) and WT tomato plants. All rolB transgenic lines were significantly shorter and produced smaller fruits when compared to WT plants (Figure 2a). The rolB expressing tomato plants had an average height of 42.3 cm as compared to 94 cm for WT plants. A reduction in apical dominance along with shorter internodal length was also observed in rolB expressing plants. Leaves were also smaller, less serrated and more oval in shape as compared to GUS control and WT. In some rolB transformed plants, mild wrinkled leaves were observed (Figure 2c). Interestingly, rolB expressing plants had profuse, long and hairy root system as compared to control (Figure 2d). The flowers were normal in size but some of them were infertile and did not produce fruits. Fruits that developed in rolB transgenic plants were smaller in size (Figure 2b) and matured earlier when compared to the fruits of control and WT plants. Number of fruits and their weight was calculated from 24 rolB expressing plants that were derived from nine independent transgenic lines. The rolB expressing tomato plants produced an average of 11 fruits per plant whereas control and WT plants produced and an average of 15 fruits per plant. Average fruit weight of rolB transgenic tomatoes was 38 g and was significantly lower than control/WT plants that had 74 g.

Bottom Line: Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum.Contrarily, transformed plants exhibited altered morphology and reduced fruit yield.In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT
Tomato (Solanum lycopersicum L.) is the second most important cultivated crop next to potato, worldwide. Tomato serves as an important source of antioxidants in human diet. Alternaria solani and Fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. The foremost objective of the present study was to generate transgenic tomato plants with rolB gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance against fungal infection. Tomato cv. Rio Grande was transformed via Agrobacterium tumefaciens harbouring rolB gene of Agrobacterium rhizogenes. rolB. Biochemical analyses showed considerable improvement in nutritional quality of transgenic tomato fruits as indicated by 62% increase in lycopene content, 225% in ascorbic acid content, 58% in total phenolics and 26% in free radical scavenging activity. Furthermore, rolB gene significantly improved the defence response of leaves of transgenic plants against two pathogenic fungal strains A. solani and F. oxysporum. Contrarily, transformed plants exhibited altered morphology and reduced fruit yield. In conclusion, rolB gene from A. rhizogenes can be used to generate transgenic tomato with increased nutritional contents of fruits as well as improved foliar tolerance against fungal pathogens.

Show MeSH
Related in: MedlinePlus