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Serological cross-reactivity between Merkel cell polyomavirus and two closely related chimpanzee polyomaviruses.

Nicol JT, Liais E, Potier R, Mazzoni E, Tognon M, Coursaget P, Touzé A - PLoS ONE (2014)

Bottom Line: Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related.In contrast, cross-reactivity was not observed between TSPyV and OraPyV1.Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

View Article: PubMed Central - PubMed

Affiliation: Université François Rabelais, Virologie Immunologie Moléculaires, Tours, France; INRA UMR 1282, Infectiologie et Santé Publique, Tours, France.

ABSTRACT
Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related. The existence of cross-reactivity between these polyomaviruses was therefore investigated. The findings indicated serological identity between the two chimpanzee polyomaviruses investigated and a high level of cross-reactivity with Merkel cell polyomavirus. In contrast, cross-reactivity was not observed between TSPyV and OraPyV1. Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

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Correlation between MCPyV and PtvPyV reactivity and between TSPyV and OraPyV1 reactivity.Correlation between ELISA reactivity of human serum samples against the different VLPs. Each point represents one serum sample. Correlation coefficients (Spearman) were determined using XLStat software.
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pone-0097030-g004: Correlation between MCPyV and PtvPyV reactivity and between TSPyV and OraPyV1 reactivity.Correlation between ELISA reactivity of human serum samples against the different VLPs. Each point represents one serum sample. Correlation coefficients (Spearman) were determined using XLStat software.

Mentions: Of the 828 subjects investigated, 433 (52.3%) were found to be seropositive for PtvPyV1 and 462 (55.8%) for PtvPyV2. It should be noted that 429 subjects were found to be positive for both PtvPyV1 and PtvPyV2, while the ELISA OD value was found to be close to the cut-off value for the remaining 37 discordant results. In addition, the positive sera for PtvPyV were also found to be positive for MCPyV (Figure 3). Correlation analysis between PtvPyV1, PtvPyV2 and MCPyV reactivity was performed with the non-parametric Spearman test (Figure 4). Serological identity between PtvPyV1 and PtvPyV2 was observed (RS = 0.970, p<1.10−4) and a strong correlation was found between MCPyV and the two PtvPyVs (RS = 0.686, p<1.10−4, and RS = 0.711, p<1.10−4).


Serological cross-reactivity between Merkel cell polyomavirus and two closely related chimpanzee polyomaviruses.

Nicol JT, Liais E, Potier R, Mazzoni E, Tognon M, Coursaget P, Touzé A - PLoS ONE (2014)

Correlation between MCPyV and PtvPyV reactivity and between TSPyV and OraPyV1 reactivity.Correlation between ELISA reactivity of human serum samples against the different VLPs. Each point represents one serum sample. Correlation coefficients (Spearman) were determined using XLStat software.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016208&req=5

pone-0097030-g004: Correlation between MCPyV and PtvPyV reactivity and between TSPyV and OraPyV1 reactivity.Correlation between ELISA reactivity of human serum samples against the different VLPs. Each point represents one serum sample. Correlation coefficients (Spearman) were determined using XLStat software.
Mentions: Of the 828 subjects investigated, 433 (52.3%) were found to be seropositive for PtvPyV1 and 462 (55.8%) for PtvPyV2. It should be noted that 429 subjects were found to be positive for both PtvPyV1 and PtvPyV2, while the ELISA OD value was found to be close to the cut-off value for the remaining 37 discordant results. In addition, the positive sera for PtvPyV were also found to be positive for MCPyV (Figure 3). Correlation analysis between PtvPyV1, PtvPyV2 and MCPyV reactivity was performed with the non-parametric Spearman test (Figure 4). Serological identity between PtvPyV1 and PtvPyV2 was observed (RS = 0.970, p<1.10−4) and a strong correlation was found between MCPyV and the two PtvPyVs (RS = 0.686, p<1.10−4, and RS = 0.711, p<1.10−4).

Bottom Line: Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related.In contrast, cross-reactivity was not observed between TSPyV and OraPyV1.Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

View Article: PubMed Central - PubMed

Affiliation: Université François Rabelais, Virologie Immunologie Moléculaires, Tours, France; INRA UMR 1282, Infectiologie et Santé Publique, Tours, France.

ABSTRACT
Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related. The existence of cross-reactivity between these polyomaviruses was therefore investigated. The findings indicated serological identity between the two chimpanzee polyomaviruses investigated and a high level of cross-reactivity with Merkel cell polyomavirus. In contrast, cross-reactivity was not observed between TSPyV and OraPyV1. Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

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