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Serological cross-reactivity between Merkel cell polyomavirus and two closely related chimpanzee polyomaviruses.

Nicol JT, Liais E, Potier R, Mazzoni E, Tognon M, Coursaget P, Touzé A - PLoS ONE (2014)

Bottom Line: Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related.In contrast, cross-reactivity was not observed between TSPyV and OraPyV1.Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

View Article: PubMed Central - PubMed

Affiliation: Université François Rabelais, Virologie Immunologie Moléculaires, Tours, France; INRA UMR 1282, Infectiologie et Santé Publique, Tours, France.

ABSTRACT
Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related. The existence of cross-reactivity between these polyomaviruses was therefore investigated. The findings indicated serological identity between the two chimpanzee polyomaviruses investigated and a high level of cross-reactivity with Merkel cell polyomavirus. In contrast, cross-reactivity was not observed between TSPyV and OraPyV1. Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

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Phylogenetic relationships between human and closely related simian polyomaviruses based on VP1 protein.Human polyomaviruses are in black and simian polyomaviruses are in gray. Sequence accession numbers used are NC_001538 for BKPyV, NC_001699 for JCPyV, NC_009238 for KIPyV, NC_009539 for WuPyV, NC_010277 for MCPyV, NC_014406 for HPyV6, NC_014407 for HPyV7, NC_014361 for TSPyV, HQ696595 for HPyV9, NC_018102 for MWPyV, JX463183 for STLPyV, JX308829 for HPyV12, NC_001669 for SV40, NC_004763 for LPyV, AY691168 for ChPyV, HQ385747 for PtvPyV1b, HQ385750 for PtvPyV2c, FN356900 for OraPyV1 and FN356901 for OraPyV2.
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pone-0097030-g001: Phylogenetic relationships between human and closely related simian polyomaviruses based on VP1 protein.Human polyomaviruses are in black and simian polyomaviruses are in gray. Sequence accession numbers used are NC_001538 for BKPyV, NC_001699 for JCPyV, NC_009238 for KIPyV, NC_009539 for WuPyV, NC_010277 for MCPyV, NC_014406 for HPyV6, NC_014407 for HPyV7, NC_014361 for TSPyV, HQ696595 for HPyV9, NC_018102 for MWPyV, JX463183 for STLPyV, JX308829 for HPyV12, NC_001669 for SV40, NC_004763 for LPyV, AY691168 for ChPyV, HQ385747 for PtvPyV1b, HQ385750 for PtvPyV2c, FN356900 for OraPyV1 and FN356901 for OraPyV2.

Mentions: As MCPyV is phylogenitically close to recently discovered chimpanzee polyomaviruses (PtvPyV1 and PtvPyV2 [26]) and similarly TSPyV is closely related to orangutan polyomavirus 1 (OraPyV1 [27]) (Figure 1), we investigated in this study the possible existence of cross-reactivity between these closely related polyomaviruses.


Serological cross-reactivity between Merkel cell polyomavirus and two closely related chimpanzee polyomaviruses.

Nicol JT, Liais E, Potier R, Mazzoni E, Tognon M, Coursaget P, Touzé A - PLoS ONE (2014)

Phylogenetic relationships between human and closely related simian polyomaviruses based on VP1 protein.Human polyomaviruses are in black and simian polyomaviruses are in gray. Sequence accession numbers used are NC_001538 for BKPyV, NC_001699 for JCPyV, NC_009238 for KIPyV, NC_009539 for WuPyV, NC_010277 for MCPyV, NC_014406 for HPyV6, NC_014407 for HPyV7, NC_014361 for TSPyV, HQ696595 for HPyV9, NC_018102 for MWPyV, JX463183 for STLPyV, JX308829 for HPyV12, NC_001669 for SV40, NC_004763 for LPyV, AY691168 for ChPyV, HQ385747 for PtvPyV1b, HQ385750 for PtvPyV2c, FN356900 for OraPyV1 and FN356901 for OraPyV2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016208&req=5

pone-0097030-g001: Phylogenetic relationships between human and closely related simian polyomaviruses based on VP1 protein.Human polyomaviruses are in black and simian polyomaviruses are in gray. Sequence accession numbers used are NC_001538 for BKPyV, NC_001699 for JCPyV, NC_009238 for KIPyV, NC_009539 for WuPyV, NC_010277 for MCPyV, NC_014406 for HPyV6, NC_014407 for HPyV7, NC_014361 for TSPyV, HQ696595 for HPyV9, NC_018102 for MWPyV, JX463183 for STLPyV, JX308829 for HPyV12, NC_001669 for SV40, NC_004763 for LPyV, AY691168 for ChPyV, HQ385747 for PtvPyV1b, HQ385750 for PtvPyV2c, FN356900 for OraPyV1 and FN356901 for OraPyV2.
Mentions: As MCPyV is phylogenitically close to recently discovered chimpanzee polyomaviruses (PtvPyV1 and PtvPyV2 [26]) and similarly TSPyV is closely related to orangutan polyomavirus 1 (OraPyV1 [27]) (Figure 1), we investigated in this study the possible existence of cross-reactivity between these closely related polyomaviruses.

Bottom Line: Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related.In contrast, cross-reactivity was not observed between TSPyV and OraPyV1.Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

View Article: PubMed Central - PubMed

Affiliation: Université François Rabelais, Virologie Immunologie Moléculaires, Tours, France; INRA UMR 1282, Infectiologie et Santé Publique, Tours, France.

ABSTRACT
Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related. The existence of cross-reactivity between these polyomaviruses was therefore investigated. The findings indicated serological identity between the two chimpanzee polyomaviruses investigated and a high level of cross-reactivity with Merkel cell polyomavirus. In contrast, cross-reactivity was not observed between TSPyV and OraPyV1. Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.

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