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Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources.

Santamauro F, Whiffin FM, Scott RJ, Chuck CJ - Biotechnol Biofuels (2014)

Bottom Line: This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes.We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source.M. pulcherrima also grows well in a minimal media containing no yeast extract.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK. R.J.Scott@bath.ac.uk.

ABSTRACT

Background: The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production.

Results: Cultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, 'pulcherrima' cell type. This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500 L, open air, tank reactor without any significant contamination.

Conclusions: The production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.

No MeSH data available.


Related in: MedlinePlus

Total biomass and lipid produced (shaded area) using an optimised media.
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Figure 5: Total biomass and lipid produced (shaded area) using an optimised media.

Mentions: Following these promising results we modified SMP to optimise lipid accumulation. The new medium, optimised M. pulcherrima (OMP) medium lacked manganese and iron, and had lower levels of sulphur. Cultures grown in this medium achieved higher lipid content whilst retaining biomass yields of around 7 g/L. In a typical experiment M. pulcherrima was grown in OMP at 25°C for the first 3 days, than at 15°C for a further 12 days. After 15 days a 7.4 g⋅L-1 biomass yield was achieved, which had a total lipid content of 40% (Figure 5). This demonstrates that with an appropriate combination of medium and temperature regime, the oil productivity of M. pulcherrima is well into the oleaginous range.


Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources.

Santamauro F, Whiffin FM, Scott RJ, Chuck CJ - Biotechnol Biofuels (2014)

Total biomass and lipid produced (shaded area) using an optimised media.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016040&req=5

Figure 5: Total biomass and lipid produced (shaded area) using an optimised media.
Mentions: Following these promising results we modified SMP to optimise lipid accumulation. The new medium, optimised M. pulcherrima (OMP) medium lacked manganese and iron, and had lower levels of sulphur. Cultures grown in this medium achieved higher lipid content whilst retaining biomass yields of around 7 g/L. In a typical experiment M. pulcherrima was grown in OMP at 25°C for the first 3 days, than at 15°C for a further 12 days. After 15 days a 7.4 g⋅L-1 biomass yield was achieved, which had a total lipid content of 40% (Figure 5). This demonstrates that with an appropriate combination of medium and temperature regime, the oil productivity of M. pulcherrima is well into the oleaginous range.

Bottom Line: This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes.We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source.M. pulcherrima also grows well in a minimal media containing no yeast extract.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK. R.J.Scott@bath.ac.uk.

ABSTRACT

Background: The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production.

Results: Cultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, 'pulcherrima' cell type. This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500 L, open air, tank reactor without any significant contamination.

Conclusions: The production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.

No MeSH data available.


Related in: MedlinePlus