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Interleukin-27 is differentially associated with HIV viral load and CD4+ T cell counts in therapy-naïve HIV-mono-infected and HIV/HCV-co-infected Chinese.

He L, Zhao J, Wang MH, Siu KK, Gan YX, Chen L, Zee BC, Yang L, Kung HF, Yang ZR, He ML - PLoS ONE (2014)

Bottom Line: Human Immunodeficiency Virus (HIV) infection and the resultant Acquired Immunodeficiency Syndrome (AIDS) epidemic are major global health challenges; hepatitis C virus (HCV) co-infection has made the HIV/AIDS epidemic even worse.We show that: plasma IL-27 level was significantly upregulated in HIV-mono-infected and HIV/HCV-co-infected Chinese; HIV viral load was negatively correlated with IL-27 titer in HIV-mono-infected subjects whereas the relationship was opposite in HIV/HCV-co-infected subjects; and the relationships between HIV viral loads, IL-27 titers and CD4+ T cell counts in the HIV mono-infection and HIV/HCV co-infection groups were dramatically different.Overall, our results suggest that IL-27 differs in treatment-naïve groups with HIV mono-infections and HIV/HCV co-infections, thereby providing critical information to be considered when caring and treating those with HIV mono-infection and HIV/HCV co-infection.

View Article: PubMed Central - PubMed

Affiliation: Stanley Ho Center for Emerging Infectious Diseases, School of Public Health and Primary Care, the Chinese University of Hong Kong, Hong Kong SAR, China.

ABSTRACT
Human Immunodeficiency Virus (HIV) infection and the resultant Acquired Immunodeficiency Syndrome (AIDS) epidemic are major global health challenges; hepatitis C virus (HCV) co-infection has made the HIV/AIDS epidemic even worse. Interleukin-27 (IL-27), a cytokine which inhibits HIV and HCV replication in vitro, associates with HIV infection and HIV/HCV co-infection in clinical settings. However, the impact of HIV and HCV viral loads on plasma IL-27 expression levels has not been well characterized. In this study, 155 antiretroviral therapy-naïve Chinese were recruited. Among them 80 were HIV- and HCV-negative healthy controls, 45 were HIV-mono-infected and 30 were HIV/HCV-co-infected. Plasma level HIV, HCV, IL-27 and CD4+ number were counted and their correlation, regression relationships were explored. We show that: plasma IL-27 level was significantly upregulated in HIV-mono-infected and HIV/HCV-co-infected Chinese; HIV viral load was negatively correlated with IL-27 titer in HIV-mono-infected subjects whereas the relationship was opposite in HIV/HCV-co-infected subjects; and the relationships between HIV viral loads, IL-27 titers and CD4+ T cell counts in the HIV mono-infection and HIV/HCV co-infection groups were dramatically different. Overall, our results suggest that IL-27 differs in treatment-naïve groups with HIV mono-infections and HIV/HCV co-infections, thereby providing critical information to be considered when caring and treating those with HIV mono-infection and HIV/HCV co-infection.

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A. Linear regression of IL-27 on HIV viral load in low HIV-mono-infected subjects. In HIV-mono-infected individuals, the IL-27 had a negative relationship with natural log of HIV viral count. The linear regresion estimate was IL27  = −80.1 log(HIV) +1,401.8, and p-value  = 0.0485. B. Linear regression of IL-27 on HIV viral load in HIV/HCV-co-infected subjects. Contrary to the significant negative relation resulting in the HIV-mono-infected group, in the HIV/HCV-co-infected individuals the IL-27 had a positive relationship with the natural log of HIV viral count. The linear regresion estimate was IL27  = 57.59 log(HIV) +97.73, and p = 0.0495.
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pone-0096792-g004: A. Linear regression of IL-27 on HIV viral load in low HIV-mono-infected subjects. In HIV-mono-infected individuals, the IL-27 had a negative relationship with natural log of HIV viral count. The linear regresion estimate was IL27  = −80.1 log(HIV) +1,401.8, and p-value  = 0.0485. B. Linear regression of IL-27 on HIV viral load in HIV/HCV-co-infected subjects. Contrary to the significant negative relation resulting in the HIV-mono-infected group, in the HIV/HCV-co-infected individuals the IL-27 had a positive relationship with the natural log of HIV viral count. The linear regresion estimate was IL27  = 57.59 log(HIV) +97.73, and p = 0.0495.

Mentions: HIV was taken the natural log transformation for variance stablization, while IL-27 titers had a normal distribution and no adjustment is needed. In the mono-infected group, the IL-27 titers correlated to log HIV viral load negatively (r = −0.04, p = 0.3). In the low HIV-mono-infected group (35 subjects), IL-27 level negatively correlated with log HIV titers significantly (r = −0.34, p = 0.024). A scatter plot of IL-27 against log HIV was in Figure 4A (low HIV group); the regression p-value was 0.0485. On the contrary, in the co-infected group, the same pair of quantities had a significant positive correlation (r = 0.36, p = 0.025). The scatter plot was in Figure 4B, and regression p-value was 0.0495. In both the low HCV group and the low HIV group of the co-infected, the IL-27 and HIV titers had a positive correlation: the low HIV group had 23 subjects; the correlation co-efficient was 0.20 and the p-value was 0.18; the low HCV group had 25 subjects and the correlation co-efficient was 0.38 and the p-value was 0.029. In sum, the IL-27 level and the HIV viral load had a negative relationship in the HIV-mono-infected individuals and a positive relationship in the HIV/HCV-co-infected individuals.


Interleukin-27 is differentially associated with HIV viral load and CD4+ T cell counts in therapy-naïve HIV-mono-infected and HIV/HCV-co-infected Chinese.

He L, Zhao J, Wang MH, Siu KK, Gan YX, Chen L, Zee BC, Yang L, Kung HF, Yang ZR, He ML - PLoS ONE (2014)

A. Linear regression of IL-27 on HIV viral load in low HIV-mono-infected subjects. In HIV-mono-infected individuals, the IL-27 had a negative relationship with natural log of HIV viral count. The linear regresion estimate was IL27  = −80.1 log(HIV) +1,401.8, and p-value  = 0.0485. B. Linear regression of IL-27 on HIV viral load in HIV/HCV-co-infected subjects. Contrary to the significant negative relation resulting in the HIV-mono-infected group, in the HIV/HCV-co-infected individuals the IL-27 had a positive relationship with the natural log of HIV viral count. The linear regresion estimate was IL27  = 57.59 log(HIV) +97.73, and p = 0.0495.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016030&req=5

pone-0096792-g004: A. Linear regression of IL-27 on HIV viral load in low HIV-mono-infected subjects. In HIV-mono-infected individuals, the IL-27 had a negative relationship with natural log of HIV viral count. The linear regresion estimate was IL27  = −80.1 log(HIV) +1,401.8, and p-value  = 0.0485. B. Linear regression of IL-27 on HIV viral load in HIV/HCV-co-infected subjects. Contrary to the significant negative relation resulting in the HIV-mono-infected group, in the HIV/HCV-co-infected individuals the IL-27 had a positive relationship with the natural log of HIV viral count. The linear regresion estimate was IL27  = 57.59 log(HIV) +97.73, and p = 0.0495.
Mentions: HIV was taken the natural log transformation for variance stablization, while IL-27 titers had a normal distribution and no adjustment is needed. In the mono-infected group, the IL-27 titers correlated to log HIV viral load negatively (r = −0.04, p = 0.3). In the low HIV-mono-infected group (35 subjects), IL-27 level negatively correlated with log HIV titers significantly (r = −0.34, p = 0.024). A scatter plot of IL-27 against log HIV was in Figure 4A (low HIV group); the regression p-value was 0.0485. On the contrary, in the co-infected group, the same pair of quantities had a significant positive correlation (r = 0.36, p = 0.025). The scatter plot was in Figure 4B, and regression p-value was 0.0495. In both the low HCV group and the low HIV group of the co-infected, the IL-27 and HIV titers had a positive correlation: the low HIV group had 23 subjects; the correlation co-efficient was 0.20 and the p-value was 0.18; the low HCV group had 25 subjects and the correlation co-efficient was 0.38 and the p-value was 0.029. In sum, the IL-27 level and the HIV viral load had a negative relationship in the HIV-mono-infected individuals and a positive relationship in the HIV/HCV-co-infected individuals.

Bottom Line: Human Immunodeficiency Virus (HIV) infection and the resultant Acquired Immunodeficiency Syndrome (AIDS) epidemic are major global health challenges; hepatitis C virus (HCV) co-infection has made the HIV/AIDS epidemic even worse.We show that: plasma IL-27 level was significantly upregulated in HIV-mono-infected and HIV/HCV-co-infected Chinese; HIV viral load was negatively correlated with IL-27 titer in HIV-mono-infected subjects whereas the relationship was opposite in HIV/HCV-co-infected subjects; and the relationships between HIV viral loads, IL-27 titers and CD4+ T cell counts in the HIV mono-infection and HIV/HCV co-infection groups were dramatically different.Overall, our results suggest that IL-27 differs in treatment-naïve groups with HIV mono-infections and HIV/HCV co-infections, thereby providing critical information to be considered when caring and treating those with HIV mono-infection and HIV/HCV co-infection.

View Article: PubMed Central - PubMed

Affiliation: Stanley Ho Center for Emerging Infectious Diseases, School of Public Health and Primary Care, the Chinese University of Hong Kong, Hong Kong SAR, China.

ABSTRACT
Human Immunodeficiency Virus (HIV) infection and the resultant Acquired Immunodeficiency Syndrome (AIDS) epidemic are major global health challenges; hepatitis C virus (HCV) co-infection has made the HIV/AIDS epidemic even worse. Interleukin-27 (IL-27), a cytokine which inhibits HIV and HCV replication in vitro, associates with HIV infection and HIV/HCV co-infection in clinical settings. However, the impact of HIV and HCV viral loads on plasma IL-27 expression levels has not been well characterized. In this study, 155 antiretroviral therapy-naïve Chinese were recruited. Among them 80 were HIV- and HCV-negative healthy controls, 45 were HIV-mono-infected and 30 were HIV/HCV-co-infected. Plasma level HIV, HCV, IL-27 and CD4+ number were counted and their correlation, regression relationships were explored. We show that: plasma IL-27 level was significantly upregulated in HIV-mono-infected and HIV/HCV-co-infected Chinese; HIV viral load was negatively correlated with IL-27 titer in HIV-mono-infected subjects whereas the relationship was opposite in HIV/HCV-co-infected subjects; and the relationships between HIV viral loads, IL-27 titers and CD4+ T cell counts in the HIV mono-infection and HIV/HCV co-infection groups were dramatically different. Overall, our results suggest that IL-27 differs in treatment-naïve groups with HIV mono-infections and HIV/HCV co-infections, thereby providing critical information to be considered when caring and treating those with HIV mono-infection and HIV/HCV co-infection.

Show MeSH
Related in: MedlinePlus