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Hydrogen-rich saline ameliorates the retina against light-induced damage in rats.

Tian L, Zhang L, Xia F, An J, Sugita Y, Zhang Z - Med Gas Res (2013)

Bottom Line: Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining.The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage.These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Medicine, Faculty of Aerospace Medicine, Key Laboratory of Aerospace Medicine of the National Education Ministry, Fourth Military University, 17 Changle West Road, Xi'an 710032, China. zzmfmmu@gmail.com.

ABSTRACT
Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague-Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per day for 5 days, after intensive light exposure), respectively. The right eye of each rat was exposed to 5000 lux constant white light-emitting diode (LED) light for 3 h, and the left eye was covered to serve as the blank control. Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining. The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage. These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

No MeSH data available.


Related in: MedlinePlus

Morphometric analysis across the vertical meridians of retinas in SD rats reared in dim cyclic light. Black lines: unexposed to intense light; red lines: rats exposed at P56 and then maintained in darkness for 5 days; blue lines: HRS was given after light exposure; green lines: after HRS was given for 30 min, rats were exposed to light. The sample size was six rats per condition, with six ONL measurements per data point.
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Figure 4: Morphometric analysis across the vertical meridians of retinas in SD rats reared in dim cyclic light. Black lines: unexposed to intense light; red lines: rats exposed at P56 and then maintained in darkness for 5 days; blue lines: HRS was given after light exposure; green lines: after HRS was given for 30 min, rats were exposed to light. The sample size was six rats per condition, with six ONL measurements per data point.

Mentions: Figure 4 shows the ONL thickness in unexposed eyes and the eyes of the light damage, HRS prevention, and HRS treatment groups using morphometry across the vertical meridian. There were significant differences of two groups comparison between the damage group and the other groups(P < 0.001). HRS showed significant protective effect both injected to the rats either before or after the light exposure. In the light damage group, there was a large loss of photoreceptors over most of the superior half and the central portion of the inferior half. In the prevention group, the reduction of ONL thickness was restricted to the middle of the superior retina (P <0.01, compared with blank control), which previously has been identified as a sensitive region [24]. In the treatment group, did not decrease significantly after exposure to light for 5 days HRS injection, especially the sensitive region, but it did not achieve normal levels (P < 0.01, compared with blank control).


Hydrogen-rich saline ameliorates the retina against light-induced damage in rats.

Tian L, Zhang L, Xia F, An J, Sugita Y, Zhang Z - Med Gas Res (2013)

Morphometric analysis across the vertical meridians of retinas in SD rats reared in dim cyclic light. Black lines: unexposed to intense light; red lines: rats exposed at P56 and then maintained in darkness for 5 days; blue lines: HRS was given after light exposure; green lines: after HRS was given for 30 min, rats were exposed to light. The sample size was six rats per condition, with six ONL measurements per data point.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016026&req=5

Figure 4: Morphometric analysis across the vertical meridians of retinas in SD rats reared in dim cyclic light. Black lines: unexposed to intense light; red lines: rats exposed at P56 and then maintained in darkness for 5 days; blue lines: HRS was given after light exposure; green lines: after HRS was given for 30 min, rats were exposed to light. The sample size was six rats per condition, with six ONL measurements per data point.
Mentions: Figure 4 shows the ONL thickness in unexposed eyes and the eyes of the light damage, HRS prevention, and HRS treatment groups using morphometry across the vertical meridian. There were significant differences of two groups comparison between the damage group and the other groups(P < 0.001). HRS showed significant protective effect both injected to the rats either before or after the light exposure. In the light damage group, there was a large loss of photoreceptors over most of the superior half and the central portion of the inferior half. In the prevention group, the reduction of ONL thickness was restricted to the middle of the superior retina (P <0.01, compared with blank control), which previously has been identified as a sensitive region [24]. In the treatment group, did not decrease significantly after exposure to light for 5 days HRS injection, especially the sensitive region, but it did not achieve normal levels (P < 0.01, compared with blank control).

Bottom Line: Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining.The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage.These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Medicine, Faculty of Aerospace Medicine, Key Laboratory of Aerospace Medicine of the National Education Ministry, Fourth Military University, 17 Changle West Road, Xi'an 710032, China. zzmfmmu@gmail.com.

ABSTRACT
Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague-Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per day for 5 days, after intensive light exposure), respectively. The right eye of each rat was exposed to 5000 lux constant white light-emitting diode (LED) light for 3 h, and the left eye was covered to serve as the blank control. Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining. The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage. These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

No MeSH data available.


Related in: MedlinePlus