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Hydrogen-rich saline ameliorates the retina against light-induced damage in rats.

Tian L, Zhang L, Xia F, An J, Sugita Y, Zhang Z - Med Gas Res (2013)

Bottom Line: Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining.The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage.These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Medicine, Faculty of Aerospace Medicine, Key Laboratory of Aerospace Medicine of the National Education Ministry, Fourth Military University, 17 Changle West Road, Xi'an 710032, China. zzmfmmu@gmail.com.

ABSTRACT
Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague-Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per day for 5 days, after intensive light exposure), respectively. The right eye of each rat was exposed to 5000 lux constant white light-emitting diode (LED) light for 3 h, and the left eye was covered to serve as the blank control. Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining. The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage. These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

No MeSH data available.


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Annular illumination box for light-induced retinal damage in rats.
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Figure 1: Annular illumination box for light-induced retinal damage in rats.

Mentions: After adaptation for 12 h in the dark, rats were exposed to intense light randomly for each group. The right eye of rats with natural pupil size was exposed to 5000 lux white LED light for 3 h. For light exposure, the rats were kept in the light box with LED light sources in six directions and a ring-shaped apparatus inside to allow rats to move one way in an anticlockwise direction (Figure 1). The distance between the right eye of the rat and the light source was kept relatively steady, and only one rat was exposed to intense light each time to avoid disturbance between rats. The light exposure experiment was performed between 6 PM and 8 AM the next day. The rats were intraperitoneally injected with HBS (5 ml/kg) 30 min before light exposure in the prevention group or 5 days after light exposure in the treatment group. In the light damage group, medical saline was injected (5 ml/kg per day for 5 days, after intensive light exposure) instead of HRS. After light exposure, the rats were returned to the dim cyclic light environment. Five days later, electroretinography (ERG) recordings were obtained and retinal morphology was observed.


Hydrogen-rich saline ameliorates the retina against light-induced damage in rats.

Tian L, Zhang L, Xia F, An J, Sugita Y, Zhang Z - Med Gas Res (2013)

Annular illumination box for light-induced retinal damage in rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4016026&req=5

Figure 1: Annular illumination box for light-induced retinal damage in rats.
Mentions: After adaptation for 12 h in the dark, rats were exposed to intense light randomly for each group. The right eye of rats with natural pupil size was exposed to 5000 lux white LED light for 3 h. For light exposure, the rats were kept in the light box with LED light sources in six directions and a ring-shaped apparatus inside to allow rats to move one way in an anticlockwise direction (Figure 1). The distance between the right eye of the rat and the light source was kept relatively steady, and only one rat was exposed to intense light each time to avoid disturbance between rats. The light exposure experiment was performed between 6 PM and 8 AM the next day. The rats were intraperitoneally injected with HBS (5 ml/kg) 30 min before light exposure in the prevention group or 5 days after light exposure in the treatment group. In the light damage group, medical saline was injected (5 ml/kg per day for 5 days, after intensive light exposure) instead of HRS. After light exposure, the rats were returned to the dim cyclic light environment. Five days later, electroretinography (ERG) recordings were obtained and retinal morphology was observed.

Bottom Line: Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining.The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage.These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Medicine, Faculty of Aerospace Medicine, Key Laboratory of Aerospace Medicine of the National Education Ministry, Fourth Military University, 17 Changle West Road, Xi'an 710032, China. zzmfmmu@gmail.com.

ABSTRACT
Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague-Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per day for 5 days, after intensive light exposure), respectively. The right eye of each rat was exposed to 5000 lux constant white light-emitting diode (LED) light for 3 h, and the left eye was covered to serve as the blank control. Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining. The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage. These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

No MeSH data available.


Related in: MedlinePlus