Limits...
Influenza A(H5N2) virus antibodies in humans after contact with infected poultry, Taiwan, 2012.

Wu HS, Yang JR, Liu MT, Yang CH, Cheng MC, Chang FY - Emerging Infect. Dis. (2014)

Bottom Line: Six persons in Taiwan who had contact with poultry infected with influenza A(H5N2) showed seroconversion for the virus by hemagglutinin inhibition or microneutralization testing.We developed an ELISA based on nonstructural protein 1 of the virus to differentiate natural infection from cross-reactivity after vaccination; 2 persons also showed seroconversion by this test.

View Article: PubMed Central - PubMed

ABSTRACT
Six persons in Taiwan who had contact with poultry infected with influenza A(H5N2) showed seroconversion for the virus by hemagglutinin inhibition or microneutralization testing. We developed an ELISA based on nonstructural protein 1 of the virus to differentiate natural infection from cross-reactivity after vaccination; 2 persons also showed seroconversion by this test.

Show MeSH

Related in: MedlinePlus

Antibody responses to 2 influenza A(H5N2) nonstructural protein 1 (NS1) peptides for paired serum samples from: A) influenza virus–infected ferrets; B) influenza virus–infected persons; C) influenza virus–vaccinated persons; D) persons in Taiwan who had contact with infected poultry during January–March 2012 and who showed seroconversion for influenza A(H5N2) virus exposure; and E) persons in Taiwan who had contact with infected poultry during January–March 2012 and who did not show seroconversion. Responses for each group were plotted by -fold increase from to second sample against NS1-pA (circles) and NS1-pB (triangles); numbers and percentages of positive responses for each sample set are indicated above each plot. Dashed lines indicate cutoff value for defining a positive response: results for the second sample in each pair 30% higher than those for first sample.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4012807&req=5

Figure 1: Antibody responses to 2 influenza A(H5N2) nonstructural protein 1 (NS1) peptides for paired serum samples from: A) influenza virus–infected ferrets; B) influenza virus–infected persons; C) influenza virus–vaccinated persons; D) persons in Taiwan who had contact with infected poultry during January–March 2012 and who showed seroconversion for influenza A(H5N2) virus exposure; and E) persons in Taiwan who had contact with infected poultry during January–March 2012 and who did not show seroconversion. Responses for each group were plotted by -fold increase from to second sample against NS1-pA (circles) and NS1-pB (triangles); numbers and percentages of positive responses for each sample set are indicated above each plot. Dashed lines indicate cutoff value for defining a positive response: results for the second sample in each pair 30% higher than those for first sample.

Mentions: For controls, we simultaneously analyzed 3 groups of paired serum samples with seroconversion (data not shown): 1) samples from 7 ferrets infected with different influenza virus strains (H1N1, n = 3; H3N2, n = 1; H5N1 [A/Vietnam/1194/2004], n = 1; and A[H1N1]pdm09, n = 2); 2) samples from 8 persons infected with influenza A(H1N1)pdm09 virus; and 3) samples from 9 persons who received vaccinations against influenza A(H5N1) virus. The resulting NS1 antibody responses were plotted (Figure). Five (71.4%) of 7 ferrets showed positive NS1 response against NS1-pA and all against NS1-pB (Figure, panel A), which indicates that influenza virus infection can cause a measurable anti-NS1 response after virus challenge. For influenza virus–infected persons, 3 (37.5%) of 8 showed responses against NS1-pA and NS1-pB (Figure, panel B), but for vaccinated persons, 1 (11.1%) of 9 showed responses against NS1-pA and none against NS1-pB (Figure, panel C). These patterns suggest that anti-NS1 response elicited by natural infection is stronger than that induced by vaccination.


Influenza A(H5N2) virus antibodies in humans after contact with infected poultry, Taiwan, 2012.

Wu HS, Yang JR, Liu MT, Yang CH, Cheng MC, Chang FY - Emerging Infect. Dis. (2014)

Antibody responses to 2 influenza A(H5N2) nonstructural protein 1 (NS1) peptides for paired serum samples from: A) influenza virus–infected ferrets; B) influenza virus–infected persons; C) influenza virus–vaccinated persons; D) persons in Taiwan who had contact with infected poultry during January–March 2012 and who showed seroconversion for influenza A(H5N2) virus exposure; and E) persons in Taiwan who had contact with infected poultry during January–March 2012 and who did not show seroconversion. Responses for each group were plotted by -fold increase from to second sample against NS1-pA (circles) and NS1-pB (triangles); numbers and percentages of positive responses for each sample set are indicated above each plot. Dashed lines indicate cutoff value for defining a positive response: results for the second sample in each pair 30% higher than those for first sample.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4012807&req=5

Figure 1: Antibody responses to 2 influenza A(H5N2) nonstructural protein 1 (NS1) peptides for paired serum samples from: A) influenza virus–infected ferrets; B) influenza virus–infected persons; C) influenza virus–vaccinated persons; D) persons in Taiwan who had contact with infected poultry during January–March 2012 and who showed seroconversion for influenza A(H5N2) virus exposure; and E) persons in Taiwan who had contact with infected poultry during January–March 2012 and who did not show seroconversion. Responses for each group were plotted by -fold increase from to second sample against NS1-pA (circles) and NS1-pB (triangles); numbers and percentages of positive responses for each sample set are indicated above each plot. Dashed lines indicate cutoff value for defining a positive response: results for the second sample in each pair 30% higher than those for first sample.
Mentions: For controls, we simultaneously analyzed 3 groups of paired serum samples with seroconversion (data not shown): 1) samples from 7 ferrets infected with different influenza virus strains (H1N1, n = 3; H3N2, n = 1; H5N1 [A/Vietnam/1194/2004], n = 1; and A[H1N1]pdm09, n = 2); 2) samples from 8 persons infected with influenza A(H1N1)pdm09 virus; and 3) samples from 9 persons who received vaccinations against influenza A(H5N1) virus. The resulting NS1 antibody responses were plotted (Figure). Five (71.4%) of 7 ferrets showed positive NS1 response against NS1-pA and all against NS1-pB (Figure, panel A), which indicates that influenza virus infection can cause a measurable anti-NS1 response after virus challenge. For influenza virus–infected persons, 3 (37.5%) of 8 showed responses against NS1-pA and NS1-pB (Figure, panel B), but for vaccinated persons, 1 (11.1%) of 9 showed responses against NS1-pA and none against NS1-pB (Figure, panel C). These patterns suggest that anti-NS1 response elicited by natural infection is stronger than that induced by vaccination.

Bottom Line: Six persons in Taiwan who had contact with poultry infected with influenza A(H5N2) showed seroconversion for the virus by hemagglutinin inhibition or microneutralization testing.We developed an ELISA based on nonstructural protein 1 of the virus to differentiate natural infection from cross-reactivity after vaccination; 2 persons also showed seroconversion by this test.

View Article: PubMed Central - PubMed

ABSTRACT
Six persons in Taiwan who had contact with poultry infected with influenza A(H5N2) showed seroconversion for the virus by hemagglutinin inhibition or microneutralization testing. We developed an ELISA based on nonstructural protein 1 of the virus to differentiate natural infection from cross-reactivity after vaccination; 2 persons also showed seroconversion by this test.

Show MeSH
Related in: MedlinePlus