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Bone regeneration of calvarial defect using marine calcareous-derived beta-tricalcium phosphate macrospheres.

Chou J, Hao J, Kuroda S, Ben-Nissan B, Milthopre B, Otsuka M - J Tissue Eng (2014)

Bottom Line: The rate of newly formed bone was measured radiologically by X-ray and micro-computed tomography and by histologic examination.It was observed that the newly regenerated bone thickened over the course of the experiment in the beta-tricalcium phosphate group.These results showed that repair of the calvarial defect can be achieved by biomimetic beta-tricalcium phosphate macrospheres, which hold potential for application as bone grafts for bone augmentation surgeries.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, Tokyo, Japan ; Faculty of Science, University of Technology, Sydney, Sydney, NSW, Australia.

ABSTRACT
The aim of this study was to examine the bone regeneration properties of beta-tricalcium phosphate hydrothermally converted from foraminifera carbonate exoskeleton in the repair of rat calvarial defect. These natural materials possess unique interconnected porous network with uniform pore size distribution, which can be potentially advantageous. In total, 20 adult male Wistar rats received full-thickness calvarial defect with a diameter of 5 mm. The rate of newly formed bone was measured radiologically by X-ray and micro-computed tomography and by histologic examination. After 2 weeks, the beta-tricalcium phosphate group exhibited full closure of the defect site, while control group remained unrestored at the end of the 6-week experimentation. It was observed that the newly regenerated bone thickened over the course of the experiment in the beta-tricalcium phosphate group. No soft tissue reaction was observed around the beta-tricalcium phosphate implant and the rats remained healthy. These results showed that repair of the calvarial defect can be achieved by biomimetic beta-tricalcium phosphate macrospheres, which hold potential for application as bone grafts for bone augmentation surgeries.

No MeSH data available.


Related in: MedlinePlus

Software reconstructed coronal image of the center of the defect shows the empty control to achieve only minor bone restoration, while β-TCP showed defect closure by 2 weeks and thickening of the bone continuing till 6 weeks.β-TCP: beta-tricalcium phosphate.
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fig6-2041731414523441: Software reconstructed coronal image of the center of the defect shows the empty control to achieve only minor bone restoration, while β-TCP showed defect closure by 2 weeks and thickening of the bone continuing till 6 weeks.β-TCP: beta-tricalcium phosphate.

Mentions: Figure 5 shows progressive bone restoration at the defect site viewed from the top, and Figure 6 shows coronal view of the progressive bone restoration at the defect site. The restoration of bone at the defect site in the control group plateaued around 4 weeks with no significant changes observed at 6 weeks. This again is in agreement with the presented BMC and BV/TV data. It can be clearly seen from the images that in the β-TCP group, at 2 weeks, the empty space in the defect has been covered, and at 4 weeks, the bone restored was able to cover the entire defect, which again correlates with the corresponding BMC and BV/TV data.


Bone regeneration of calvarial defect using marine calcareous-derived beta-tricalcium phosphate macrospheres.

Chou J, Hao J, Kuroda S, Ben-Nissan B, Milthopre B, Otsuka M - J Tissue Eng (2014)

Software reconstructed coronal image of the center of the defect shows the empty control to achieve only minor bone restoration, while β-TCP showed defect closure by 2 weeks and thickening of the bone continuing till 6 weeks.β-TCP: beta-tricalcium phosphate.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4012694&req=5

fig6-2041731414523441: Software reconstructed coronal image of the center of the defect shows the empty control to achieve only minor bone restoration, while β-TCP showed defect closure by 2 weeks and thickening of the bone continuing till 6 weeks.β-TCP: beta-tricalcium phosphate.
Mentions: Figure 5 shows progressive bone restoration at the defect site viewed from the top, and Figure 6 shows coronal view of the progressive bone restoration at the defect site. The restoration of bone at the defect site in the control group plateaued around 4 weeks with no significant changes observed at 6 weeks. This again is in agreement with the presented BMC and BV/TV data. It can be clearly seen from the images that in the β-TCP group, at 2 weeks, the empty space in the defect has been covered, and at 4 weeks, the bone restored was able to cover the entire defect, which again correlates with the corresponding BMC and BV/TV data.

Bottom Line: The rate of newly formed bone was measured radiologically by X-ray and micro-computed tomography and by histologic examination.It was observed that the newly regenerated bone thickened over the course of the experiment in the beta-tricalcium phosphate group.These results showed that repair of the calvarial defect can be achieved by biomimetic beta-tricalcium phosphate macrospheres, which hold potential for application as bone grafts for bone augmentation surgeries.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, Tokyo, Japan ; Faculty of Science, University of Technology, Sydney, Sydney, NSW, Australia.

ABSTRACT
The aim of this study was to examine the bone regeneration properties of beta-tricalcium phosphate hydrothermally converted from foraminifera carbonate exoskeleton in the repair of rat calvarial defect. These natural materials possess unique interconnected porous network with uniform pore size distribution, which can be potentially advantageous. In total, 20 adult male Wistar rats received full-thickness calvarial defect with a diameter of 5 mm. The rate of newly formed bone was measured radiologically by X-ray and micro-computed tomography and by histologic examination. After 2 weeks, the beta-tricalcium phosphate group exhibited full closure of the defect site, while control group remained unrestored at the end of the 6-week experimentation. It was observed that the newly regenerated bone thickened over the course of the experiment in the beta-tricalcium phosphate group. No soft tissue reaction was observed around the beta-tricalcium phosphate implant and the rats remained healthy. These results showed that repair of the calvarial defect can be achieved by biomimetic beta-tricalcium phosphate macrospheres, which hold potential for application as bone grafts for bone augmentation surgeries.

No MeSH data available.


Related in: MedlinePlus