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Excretory/secretory products from Trichinella spiralis adult worms ameliorate DSS-induced colitis in mice.

Yang X, Yang Y, Wang Y, Zhan B, Gu Y, Cheng Y, Zhu X - PLoS ONE (2014)

Bottom Line: To determine the T. spiralis AES product-induced immunological response, Th1, Th2, Th17 and regulatory cytokine profiles were measured in lymphocytes isolated from colon, mesenteric lymph nodes (MLN), and the spleen of treated mice.Mice treated with AES significantly ameliorated the severity of the DSS-induced colitis indicated by the reduced disease manifestations, improved macroscopic and microscopic inflammation correlated with the up-regulation of Treg response (increased regulatory cytokines IL-10, TGF-beta and regulatory T cells) and down-regulation of pro-inflammatory cytokines (IFN-gamma, IL-6 and IL-17) in the spleens, MLN and colon of treated mice.This effect is possibly mediated by the immunomodulation of regulatory T cells to produce regulatory and anti-inflammatory cytokines and inhibit pro-inflammatory cytokines.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China; Department of Microbiology and Parasitology, Bengbu Medical College; Anhui Key Laboratory of Infection and Immunity, Bengbu, Anhui, China.

ABSTRACT

Background: Many evidences show the inverse correlation between helminth infection and allergic or autoimmune diseases. Identification and characterization of the active helminth-derived products responsible for the beneficial effects on allergic or inflammatory diseases will provide another feasible approach to treat these diseases.

Methods and findings: Colitis was induced in C57BL/6 mice by giving 3% DSS orally for 7 days. During this period, the mice were treated daily with the excretory/secretory products from T. spiralis adult worms (AES) intraperitoneally. The severity of colitis was monitored by measuring body weight, stool consistency or bleeding, colon length and inflammation. To determine the T. spiralis AES product-induced immunological response, Th1, Th2, Th17 and regulatory cytokine profiles were measured in lymphocytes isolated from colon, mesenteric lymph nodes (MLN), and the spleen of treated mice. The CD4+ CD25+ FOXP3+ regulatory T cells (Tregs) were also measured in the spleens and MLN of treated mice. Mice treated with AES significantly ameliorated the severity of the DSS-induced colitis indicated by the reduced disease manifestations, improved macroscopic and microscopic inflammation correlated with the up-regulation of Treg response (increased regulatory cytokines IL-10, TGF-beta and regulatory T cells) and down-regulation of pro-inflammatory cytokines (IFN-gamma, IL-6 and IL-17) in the spleens, MLN and colon of treated mice.

Conclusions: Our results provide direct evidences that T. spiralis AES have a therapeutic potential for alleviating inflammatory colitis in mice. This effect is possibly mediated by the immunomodulation of regulatory T cells to produce regulatory and anti-inflammatory cytokines and inhibit pro-inflammatory cytokines.

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Related in: MedlinePlus

Treatment with the T. spiralis AES reduced the levels of the DSS-induced pro-inflammatory cytokines IFN-γ, IL-6 and IL-17 in the spleens, MLN and colon lymphocytes.The data are presented as the mean ± SE. The asterisks* indicate statistical significance at P<0.05; SFU, spot-forming units; for spleens and MLN, n = 16 in each group (for IL-17, n = 12); for LPMC, n = 15 in each group (3 mice LPMC pooled).
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pone-0096454-g003: Treatment with the T. spiralis AES reduced the levels of the DSS-induced pro-inflammatory cytokines IFN-γ, IL-6 and IL-17 in the spleens, MLN and colon lymphocytes.The data are presented as the mean ± SE. The asterisks* indicate statistical significance at P<0.05; SFU, spot-forming units; for spleens and MLN, n = 16 in each group (for IL-17, n = 12); for LPMC, n = 15 in each group (3 mice LPMC pooled).

Mentions: Compared to the non-treated group (PBS), the levels of the pro-inflammatory Th1 cytokines such as IFN-γ and IL-6 were significantly increased in the spleens, MLN and colon of the mice treated with DSS (PBS-DSS) (P<0.05). Similarly, Th17 cytokine level was strikingly increased in the colons of the DSS-receiving groups, but the increase of IL-17 in spleens and MLN of the DSS-induced mice was not statistically significant compared to the non-treated mice (Fig. 3). Treatment with T. spiralis AES during the DSS induction (AES-DSS) significantly reduced the spleens, MLN and colon secreted pro-inflammatory cytokines IFN-γ and IL-6 compared to non-treated control group (PBS-DSS) (P<0.05). The IL-17 level was also significantly down-regulated by treatment with the T. spiralis AES in the colon and MLN of the mice with DSS-induced colitis (Fig. 3).


Excretory/secretory products from Trichinella spiralis adult worms ameliorate DSS-induced colitis in mice.

Yang X, Yang Y, Wang Y, Zhan B, Gu Y, Cheng Y, Zhu X - PLoS ONE (2014)

Treatment with the T. spiralis AES reduced the levels of the DSS-induced pro-inflammatory cytokines IFN-γ, IL-6 and IL-17 in the spleens, MLN and colon lymphocytes.The data are presented as the mean ± SE. The asterisks* indicate statistical significance at P<0.05; SFU, spot-forming units; for spleens and MLN, n = 16 in each group (for IL-17, n = 12); for LPMC, n = 15 in each group (3 mice LPMC pooled).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008629&req=5

pone-0096454-g003: Treatment with the T. spiralis AES reduced the levels of the DSS-induced pro-inflammatory cytokines IFN-γ, IL-6 and IL-17 in the spleens, MLN and colon lymphocytes.The data are presented as the mean ± SE. The asterisks* indicate statistical significance at P<0.05; SFU, spot-forming units; for spleens and MLN, n = 16 in each group (for IL-17, n = 12); for LPMC, n = 15 in each group (3 mice LPMC pooled).
Mentions: Compared to the non-treated group (PBS), the levels of the pro-inflammatory Th1 cytokines such as IFN-γ and IL-6 were significantly increased in the spleens, MLN and colon of the mice treated with DSS (PBS-DSS) (P<0.05). Similarly, Th17 cytokine level was strikingly increased in the colons of the DSS-receiving groups, but the increase of IL-17 in spleens and MLN of the DSS-induced mice was not statistically significant compared to the non-treated mice (Fig. 3). Treatment with T. spiralis AES during the DSS induction (AES-DSS) significantly reduced the spleens, MLN and colon secreted pro-inflammatory cytokines IFN-γ and IL-6 compared to non-treated control group (PBS-DSS) (P<0.05). The IL-17 level was also significantly down-regulated by treatment with the T. spiralis AES in the colon and MLN of the mice with DSS-induced colitis (Fig. 3).

Bottom Line: To determine the T. spiralis AES product-induced immunological response, Th1, Th2, Th17 and regulatory cytokine profiles were measured in lymphocytes isolated from colon, mesenteric lymph nodes (MLN), and the spleen of treated mice.Mice treated with AES significantly ameliorated the severity of the DSS-induced colitis indicated by the reduced disease manifestations, improved macroscopic and microscopic inflammation correlated with the up-regulation of Treg response (increased regulatory cytokines IL-10, TGF-beta and regulatory T cells) and down-regulation of pro-inflammatory cytokines (IFN-gamma, IL-6 and IL-17) in the spleens, MLN and colon of treated mice.This effect is possibly mediated by the immunomodulation of regulatory T cells to produce regulatory and anti-inflammatory cytokines and inhibit pro-inflammatory cytokines.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China; Department of Microbiology and Parasitology, Bengbu Medical College; Anhui Key Laboratory of Infection and Immunity, Bengbu, Anhui, China.

ABSTRACT

Background: Many evidences show the inverse correlation between helminth infection and allergic or autoimmune diseases. Identification and characterization of the active helminth-derived products responsible for the beneficial effects on allergic or inflammatory diseases will provide another feasible approach to treat these diseases.

Methods and findings: Colitis was induced in C57BL/6 mice by giving 3% DSS orally for 7 days. During this period, the mice were treated daily with the excretory/secretory products from T. spiralis adult worms (AES) intraperitoneally. The severity of colitis was monitored by measuring body weight, stool consistency or bleeding, colon length and inflammation. To determine the T. spiralis AES product-induced immunological response, Th1, Th2, Th17 and regulatory cytokine profiles were measured in lymphocytes isolated from colon, mesenteric lymph nodes (MLN), and the spleen of treated mice. The CD4+ CD25+ FOXP3+ regulatory T cells (Tregs) were also measured in the spleens and MLN of treated mice. Mice treated with AES significantly ameliorated the severity of the DSS-induced colitis indicated by the reduced disease manifestations, improved macroscopic and microscopic inflammation correlated with the up-regulation of Treg response (increased regulatory cytokines IL-10, TGF-beta and regulatory T cells) and down-regulation of pro-inflammatory cytokines (IFN-gamma, IL-6 and IL-17) in the spleens, MLN and colon of treated mice.

Conclusions: Our results provide direct evidences that T. spiralis AES have a therapeutic potential for alleviating inflammatory colitis in mice. This effect is possibly mediated by the immunomodulation of regulatory T cells to produce regulatory and anti-inflammatory cytokines and inhibit pro-inflammatory cytokines.

Show MeSH
Related in: MedlinePlus