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Crude oil treatment leads to shift of bacterial communities in soils from the deep active layer and upper permafrost along the China-Russia Crude Oil Pipeline route.

Yang S, Wen X, Zhao L, Shi Y, Jin H - PLoS ONE (2014)

Bottom Line: The result showed that bacterial diversity was reduced after 8-weeks contamination.The contamination led to enrichment of indigenous bacterial taxa like Novosphingobium, Sphingobium, Caulobacter, Phenylobacterium, Alicylobacillus and Arthrobacter, which are generally capable of degrading polycyclic aromatic hydrocarbons (PAHs).The community shift highlighted the resilience of PAH degraders and their potential for in-situ degradation of crude oil under favorable conditions in the deep soils.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Frozen Soils Engineering (SKLFSE), Cold and Arid Regions Environmental and Engineering Research Institute (CAREERI), Chinese Academy of Sciences, Lanzhou, Gansu, China.

ABSTRACT
The buried China-Russia Crude Oil Pipeline (CRCOP) across the permafrost-associated cold ecosystem in northeastern China carries a risk of contamination to the deep active layers and upper permafrost in case of accidental rupture of the embedded pipeline or migration of oil spills. As many soil microbes are capable of degrading petroleum, knowledge about the intrinsic degraders and the microbial dynamics in the deep subsurface could extend our understanding of the application of in-situ bioremediation. In this study, an experiment was conducted to investigate the bacterial communities in response to simulated contamination to deep soil samples by using 454 pyrosequencing amplicons. The result showed that bacterial diversity was reduced after 8-weeks contamination. A shift in bacterial community composition was apparent in crude oil-amended soils with Proteobacteria (esp. α-subdivision) being the dominant phylum, together with Actinobacteria and Firmicutes. The contamination led to enrichment of indigenous bacterial taxa like Novosphingobium, Sphingobium, Caulobacter, Phenylobacterium, Alicylobacillus and Arthrobacter, which are generally capable of degrading polycyclic aromatic hydrocarbons (PAHs). The community shift highlighted the resilience of PAH degraders and their potential for in-situ degradation of crude oil under favorable conditions in the deep soils.

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Correspondence analysis (CA) (left) and non-metric multidimensional scaling (NMDS) ordination (right) for bacterial communities of both innate and contaminated samples.Note: The configuration of NMDs was rotated to have the greatest variation along the first axis.
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pone-0096552-g005: Correspondence analysis (CA) (left) and non-metric multidimensional scaling (NMDS) ordination (right) for bacterial communities of both innate and contaminated samples.Note: The configuration of NMDs was rotated to have the greatest variation along the first axis.

Mentions: The CA ordination showed that the indigenous bacterial profiles either from the deep active layer or permafrost mapped together in the upper left of the ordination space, whereas the communities after crude oil treatment scattered away from them. Moreover, the oiled samples clustered as two separate groups. One is composed of the samples from the south (TY and JQ) and the other is made up of soils from north parts (WN and WL) along the CRCOP pipeline (Fig. 5 left). The NMDs ordination based on the Bray-Curtis distance (Fig. 5, right) generally agree with the CA analysis, showing clear bacterial shifts of bacterial populations in response to oil treatment. The oiled samples in ordination appeared to shift away from the corresponding clean/intact samples which grouped in the left part. Like the CA chart, the north samples (WN and WL) clustered together, while the south samples (TY and JQ) grouped more closely to each other, although not as strong as in the CA ordination. Furthermore, the overall microbial community structures were distinct site by site, but samples after treatment were generally clustered by geographic locations.


Crude oil treatment leads to shift of bacterial communities in soils from the deep active layer and upper permafrost along the China-Russia Crude Oil Pipeline route.

Yang S, Wen X, Zhao L, Shi Y, Jin H - PLoS ONE (2014)

Correspondence analysis (CA) (left) and non-metric multidimensional scaling (NMDS) ordination (right) for bacterial communities of both innate and contaminated samples.Note: The configuration of NMDs was rotated to have the greatest variation along the first axis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008593&req=5

pone-0096552-g005: Correspondence analysis (CA) (left) and non-metric multidimensional scaling (NMDS) ordination (right) for bacterial communities of both innate and contaminated samples.Note: The configuration of NMDs was rotated to have the greatest variation along the first axis.
Mentions: The CA ordination showed that the indigenous bacterial profiles either from the deep active layer or permafrost mapped together in the upper left of the ordination space, whereas the communities after crude oil treatment scattered away from them. Moreover, the oiled samples clustered as two separate groups. One is composed of the samples from the south (TY and JQ) and the other is made up of soils from north parts (WN and WL) along the CRCOP pipeline (Fig. 5 left). The NMDs ordination based on the Bray-Curtis distance (Fig. 5, right) generally agree with the CA analysis, showing clear bacterial shifts of bacterial populations in response to oil treatment. The oiled samples in ordination appeared to shift away from the corresponding clean/intact samples which grouped in the left part. Like the CA chart, the north samples (WN and WL) clustered together, while the south samples (TY and JQ) grouped more closely to each other, although not as strong as in the CA ordination. Furthermore, the overall microbial community structures were distinct site by site, but samples after treatment were generally clustered by geographic locations.

Bottom Line: The result showed that bacterial diversity was reduced after 8-weeks contamination.The contamination led to enrichment of indigenous bacterial taxa like Novosphingobium, Sphingobium, Caulobacter, Phenylobacterium, Alicylobacillus and Arthrobacter, which are generally capable of degrading polycyclic aromatic hydrocarbons (PAHs).The community shift highlighted the resilience of PAH degraders and their potential for in-situ degradation of crude oil under favorable conditions in the deep soils.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Frozen Soils Engineering (SKLFSE), Cold and Arid Regions Environmental and Engineering Research Institute (CAREERI), Chinese Academy of Sciences, Lanzhou, Gansu, China.

ABSTRACT
The buried China-Russia Crude Oil Pipeline (CRCOP) across the permafrost-associated cold ecosystem in northeastern China carries a risk of contamination to the deep active layers and upper permafrost in case of accidental rupture of the embedded pipeline or migration of oil spills. As many soil microbes are capable of degrading petroleum, knowledge about the intrinsic degraders and the microbial dynamics in the deep subsurface could extend our understanding of the application of in-situ bioremediation. In this study, an experiment was conducted to investigate the bacterial communities in response to simulated contamination to deep soil samples by using 454 pyrosequencing amplicons. The result showed that bacterial diversity was reduced after 8-weeks contamination. A shift in bacterial community composition was apparent in crude oil-amended soils with Proteobacteria (esp. α-subdivision) being the dominant phylum, together with Actinobacteria and Firmicutes. The contamination led to enrichment of indigenous bacterial taxa like Novosphingobium, Sphingobium, Caulobacter, Phenylobacterium, Alicylobacillus and Arthrobacter, which are generally capable of degrading polycyclic aromatic hydrocarbons (PAHs). The community shift highlighted the resilience of PAH degraders and their potential for in-situ degradation of crude oil under favorable conditions in the deep soils.

Show MeSH
Related in: MedlinePlus