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Characterization of multidrug-resistant Salmonella enterica serovars Indiana and Enteritidis from chickens in Eastern China.

Lu Y, Zhao H, Sun J, Liu Y, Zhou X, Beier RC, Wu G, Hou X - PLoS ONE (2014)

Bottom Line: Antimicrobial sensitivity results showed that the isolates were generally resistant to sulfamethoxazole, ampicillin, tetracycline, doxycycline and trimethoprim, and 95% of the isolates sensitive to amikacin and polymyxin.The increasing frequency of multidrug resistance rate in Salmonella was associated with increasing prevalence of int1 genes (rs = 0.938, P = 0.00039).Some isolates with similar antimicrobial resistance patterns represented a variety of Salmonella enterica serovar Indiana genotypes, and were derived from a different clone.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.

ABSTRACT
A total of 310 Salmonella isolates were isolated from 6 broiler farms in Eastern China, serotyped according to the Kauffmann-White classification. All isolates were examined for susceptibility to 17 commonly used antimicrobial agents, representative isolates were examined for resistance genes and class I integrons using PCR technology. Clonality was determined by pulsed-field gel electrophoresis (PFGE). There were two serotypes detected in the 310 Salmonella strains, which included 133 Salmonella enterica serovar Indiana isolates and 177 Salmonella enterica serovar Enteritidis isolates. Antimicrobial sensitivity results showed that the isolates were generally resistant to sulfamethoxazole, ampicillin, tetracycline, doxycycline and trimethoprim, and 95% of the isolates sensitive to amikacin and polymyxin. Among all Salmonella enterica serovar Indiana isolates, 108 (81.2%) possessed the blaTEM, floR, tetA, strA and aac (6')-Ib-cr resistance genes. The detected carriage rate of class 1 integrons was 66.5% (206/310), with 6 strains carrying gene integron cassette dfr17-aadA5. The increasing frequency of multidrug resistance rate in Salmonella was associated with increasing prevalence of int1 genes (rs = 0.938, P = 0.00039). The int1, blaTEM, floR, tetA, strA and aac (6')-Ib-cr positive Salmonella enterica serovar Indiana isolates showed five major patterns as determined by PFGE. Most isolates exhibited the common PFGE patterns found from the chicken farms, suggesting that many multidrug-resistant isolates of Salmonella enterica serovar Indiana prevailed in these sources. Some isolates with similar antimicrobial resistance patterns represented a variety of Salmonella enterica serovar Indiana genotypes, and were derived from a different clone.

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PFGE pattern of 104 Salmonella enterica serovars Indiana.Chromosomal DNA of 104 Salmonella enterica serovar Indiana isolates carrying the int1, blaTEM, floR, tetA, strA, and aac(6′)-Ib-cr genes were digested with the restriction enzyme XbaI and then subjected to PFGE analysis. The results showed five major patterns as determined by PFGE.
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pone-0096050-g002: PFGE pattern of 104 Salmonella enterica serovars Indiana.Chromosomal DNA of 104 Salmonella enterica serovar Indiana isolates carrying the int1, blaTEM, floR, tetA, strA, and aac(6′)-Ib-cr genes were digested with the restriction enzyme XbaI and then subjected to PFGE analysis. The results showed five major patterns as determined by PFGE.

Mentions: The int1, blaTEM, floR, tetA, strA and aac (6') -Ib-cr positive Salmonella enterica serovar Indiana isolates showed five major patterns as determined by PFGE (Figure 2). Most isolates had showed the common PFGE patterns in all 6 chicken farms, suggesting that many multidrug resistant Salmonella enterica serovar Indiana isolates prevailed in the three sources. Some were not derived from a specific clone, but represented a variety of different genotypes.


Characterization of multidrug-resistant Salmonella enterica serovars Indiana and Enteritidis from chickens in Eastern China.

Lu Y, Zhao H, Sun J, Liu Y, Zhou X, Beier RC, Wu G, Hou X - PLoS ONE (2014)

PFGE pattern of 104 Salmonella enterica serovars Indiana.Chromosomal DNA of 104 Salmonella enterica serovar Indiana isolates carrying the int1, blaTEM, floR, tetA, strA, and aac(6′)-Ib-cr genes were digested with the restriction enzyme XbaI and then subjected to PFGE analysis. The results showed five major patterns as determined by PFGE.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008530&req=5

pone-0096050-g002: PFGE pattern of 104 Salmonella enterica serovars Indiana.Chromosomal DNA of 104 Salmonella enterica serovar Indiana isolates carrying the int1, blaTEM, floR, tetA, strA, and aac(6′)-Ib-cr genes were digested with the restriction enzyme XbaI and then subjected to PFGE analysis. The results showed five major patterns as determined by PFGE.
Mentions: The int1, blaTEM, floR, tetA, strA and aac (6') -Ib-cr positive Salmonella enterica serovar Indiana isolates showed five major patterns as determined by PFGE (Figure 2). Most isolates had showed the common PFGE patterns in all 6 chicken farms, suggesting that many multidrug resistant Salmonella enterica serovar Indiana isolates prevailed in the three sources. Some were not derived from a specific clone, but represented a variety of different genotypes.

Bottom Line: Antimicrobial sensitivity results showed that the isolates were generally resistant to sulfamethoxazole, ampicillin, tetracycline, doxycycline and trimethoprim, and 95% of the isolates sensitive to amikacin and polymyxin.The increasing frequency of multidrug resistance rate in Salmonella was associated with increasing prevalence of int1 genes (rs = 0.938, P = 0.00039).Some isolates with similar antimicrobial resistance patterns represented a variety of Salmonella enterica serovar Indiana genotypes, and were derived from a different clone.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.

ABSTRACT
A total of 310 Salmonella isolates were isolated from 6 broiler farms in Eastern China, serotyped according to the Kauffmann-White classification. All isolates were examined for susceptibility to 17 commonly used antimicrobial agents, representative isolates were examined for resistance genes and class I integrons using PCR technology. Clonality was determined by pulsed-field gel electrophoresis (PFGE). There were two serotypes detected in the 310 Salmonella strains, which included 133 Salmonella enterica serovar Indiana isolates and 177 Salmonella enterica serovar Enteritidis isolates. Antimicrobial sensitivity results showed that the isolates were generally resistant to sulfamethoxazole, ampicillin, tetracycline, doxycycline and trimethoprim, and 95% of the isolates sensitive to amikacin and polymyxin. Among all Salmonella enterica serovar Indiana isolates, 108 (81.2%) possessed the blaTEM, floR, tetA, strA and aac (6')-Ib-cr resistance genes. The detected carriage rate of class 1 integrons was 66.5% (206/310), with 6 strains carrying gene integron cassette dfr17-aadA5. The increasing frequency of multidrug resistance rate in Salmonella was associated with increasing prevalence of int1 genes (rs = 0.938, P = 0.00039). The int1, blaTEM, floR, tetA, strA and aac (6')-Ib-cr positive Salmonella enterica serovar Indiana isolates showed five major patterns as determined by PFGE. Most isolates exhibited the common PFGE patterns found from the chicken farms, suggesting that many multidrug-resistant isolates of Salmonella enterica serovar Indiana prevailed in these sources. Some isolates with similar antimicrobial resistance patterns represented a variety of Salmonella enterica serovar Indiana genotypes, and were derived from a different clone.

Show MeSH
Related in: MedlinePlus