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Beneficial effects of pioglitazone and metformin in murine model of polycystic ovaries via improvement of chemerin gene up-regulation.

Kabiri N, Tabandeh MR, Tabatabaie SR - Daru (2014)

Bottom Line: Ovarian chemerin expression was analyzed by real time PCR and western blotting.Our results demonstrated that PCO induction resulted in elevation of chemerin mRNA and protein levels in ovary in concomitant with incidence of insulin resistance and increasing androgen and progesterone production.Based on data presented here we concluded that alteration of ovarian chemerin expression may has important role in PCO development and manipulation of chemerin expression or signaling by pioglitazone or metformin can be a novel therapeutic mechanism in the treatment of PCO patients by these drugs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. m.tabandeh@scu.ac.ir.

ABSTRACT

Background: Polycystic ovary syndrome (PCO) is recognized as the most common endocrinopathy in female. Chemerin is a novel adipocytokine that is expressed in ovary and upregulated in adipose tissue of obese, PCO patients. To date there is no report about the regulation of ovarian chemerin gene expression after PCO induction and treatment by insulin sensitizing drugs including pioglitazone and metformin. Thirty female rats were divided into six experimental groups with five rats in each group including control group, PCO group (i.m injection of 4 mg estradiol benzoate for 40 days), metformin treated (200 mg/kg/day for 21 days), pioglitazone treated (20 mg/kg/day, for 21 days), PCO + metformin and PCO + pioglitazone. PCO was detected by microscopic observation of vaginal smear and treatment by metformin and pioglitazone was initiated one week after that. Ovarian chemerin expression was analyzed by real time PCR and western blotting.

Results: Our results demonstrated that PCO induction resulted in elevation of chemerin mRNA and protein levels in ovary in concomitant with incidence of insulin resistance and increasing androgen and progesterone production. We observed that metformin and pioglitazone attenuated ovarian chemerin expression and improved insulin resistance and abnormal steroid production in PCO rats.

Conclusion: Based on data presented here we concluded that alteration of ovarian chemerin expression may has important role in PCO development and manipulation of chemerin expression or signaling by pioglitazone or metformin can be a novel therapeutic mechanism in the treatment of PCO patients by these drugs.

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Related in: MedlinePlus

Serum insulin levels (ug/L) in normal rats (n = 5), PCO induced rats (n = 5) and treated rats with metformin and pioglitazone (n = 5). Serum insulin level was considerably higher in PCO rats compared with that in normal animals. Treatment of PCO rats with metformin and pioglitazone decreased the over-secretion of insulin in relation to PCO untreated rats. Data were presented as the mean ± SD. Different letters denote differences among groups at P < 0.05.
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Figure 4: Serum insulin levels (ug/L) in normal rats (n = 5), PCO induced rats (n = 5) and treated rats with metformin and pioglitazone (n = 5). Serum insulin level was considerably higher in PCO rats compared with that in normal animals. Treatment of PCO rats with metformin and pioglitazone decreased the over-secretion of insulin in relation to PCO untreated rats. Data were presented as the mean ± SD. Different letters denote differences among groups at P < 0.05.

Mentions: As shown in Figures 3 and 4 plasma glucose and insulin levels were higher in the PCO induced rats (1.92 ± 0.38 fold) when compared with normal untreated group (p <0.05) after controlling for the effect of treatment. Treatment of PCO rats with metformin and pioglitazone resulted in reduction of glucose (1.36 ± 0.27) and insulin (1.82 ± 0.36) levels in relation to PCO untreated rats (p < 0.05) (Figures 3 and 4). In healthy treated groups plasma insulin and glucose levels had no differences with healthy untreated animals (Figures 3 and 4), (p > 0.05) after controlling for PCO status.


Beneficial effects of pioglitazone and metformin in murine model of polycystic ovaries via improvement of chemerin gene up-regulation.

Kabiri N, Tabandeh MR, Tabatabaie SR - Daru (2014)

Serum insulin levels (ug/L) in normal rats (n = 5), PCO induced rats (n = 5) and treated rats with metformin and pioglitazone (n = 5). Serum insulin level was considerably higher in PCO rats compared with that in normal animals. Treatment of PCO rats with metformin and pioglitazone decreased the over-secretion of insulin in relation to PCO untreated rats. Data were presented as the mean ± SD. Different letters denote differences among groups at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4008382&req=5

Figure 4: Serum insulin levels (ug/L) in normal rats (n = 5), PCO induced rats (n = 5) and treated rats with metformin and pioglitazone (n = 5). Serum insulin level was considerably higher in PCO rats compared with that in normal animals. Treatment of PCO rats with metformin and pioglitazone decreased the over-secretion of insulin in relation to PCO untreated rats. Data were presented as the mean ± SD. Different letters denote differences among groups at P < 0.05.
Mentions: As shown in Figures 3 and 4 plasma glucose and insulin levels were higher in the PCO induced rats (1.92 ± 0.38 fold) when compared with normal untreated group (p <0.05) after controlling for the effect of treatment. Treatment of PCO rats with metformin and pioglitazone resulted in reduction of glucose (1.36 ± 0.27) and insulin (1.82 ± 0.36) levels in relation to PCO untreated rats (p < 0.05) (Figures 3 and 4). In healthy treated groups plasma insulin and glucose levels had no differences with healthy untreated animals (Figures 3 and 4), (p > 0.05) after controlling for PCO status.

Bottom Line: Ovarian chemerin expression was analyzed by real time PCR and western blotting.Our results demonstrated that PCO induction resulted in elevation of chemerin mRNA and protein levels in ovary in concomitant with incidence of insulin resistance and increasing androgen and progesterone production.Based on data presented here we concluded that alteration of ovarian chemerin expression may has important role in PCO development and manipulation of chemerin expression or signaling by pioglitazone or metformin can be a novel therapeutic mechanism in the treatment of PCO patients by these drugs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. m.tabandeh@scu.ac.ir.

ABSTRACT

Background: Polycystic ovary syndrome (PCO) is recognized as the most common endocrinopathy in female. Chemerin is a novel adipocytokine that is expressed in ovary and upregulated in adipose tissue of obese, PCO patients. To date there is no report about the regulation of ovarian chemerin gene expression after PCO induction and treatment by insulin sensitizing drugs including pioglitazone and metformin. Thirty female rats were divided into six experimental groups with five rats in each group including control group, PCO group (i.m injection of 4 mg estradiol benzoate for 40 days), metformin treated (200 mg/kg/day for 21 days), pioglitazone treated (20 mg/kg/day, for 21 days), PCO + metformin and PCO + pioglitazone. PCO was detected by microscopic observation of vaginal smear and treatment by metformin and pioglitazone was initiated one week after that. Ovarian chemerin expression was analyzed by real time PCR and western blotting.

Results: Our results demonstrated that PCO induction resulted in elevation of chemerin mRNA and protein levels in ovary in concomitant with incidence of insulin resistance and increasing androgen and progesterone production. We observed that metformin and pioglitazone attenuated ovarian chemerin expression and improved insulin resistance and abnormal steroid production in PCO rats.

Conclusion: Based on data presented here we concluded that alteration of ovarian chemerin expression may has important role in PCO development and manipulation of chemerin expression or signaling by pioglitazone or metformin can be a novel therapeutic mechanism in the treatment of PCO patients by these drugs.

Show MeSH
Related in: MedlinePlus