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Bifidobacterium breve attenuates murine dextran sodium sulfate-induced colitis and increases regulatory T cell responses.

Zheng B, van Bergenhenegouwen J, Overbeek S, van de Kant HJ, Garssen J, Folkerts G, Vos P, Morgan ME, Kraneveld AD - PLoS ONE (2014)

Bottom Line: In addition, B. breve incubation was also able to reduce Th1 and increase Treg cell subsets in contrast to L. rhamnosus.In addition, intervention with B. breve led to increases of Treg and decreases of Th17 cell subsets in Peyer's patches of DSS-treated mice.B. breve modulates T cell polarization towards Th2 and Treg cell-associated responses in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Utrecht Institute for Pharmaceutical Science, Faculty of Science, Utrecht University, Utrecht, The Netherlands.

ABSTRACT
While some probiotics have shown beneficial effects on preventing or treating colitis development, others have shown no effects. In this study, we have assessed the immunomodulating effects of two probiotic strains, Lactobacillus rhamnosus (L. rhamnosus) and Bifidobacterium breve (B. breve) on T cell polarization in vitro, using human peripheral blood mononuclear cells (PBMC), and in vivo, using murine dextran sodium sulfate (DSS) colitis model. With respect to the latter, the mRNA expression of T cell subset-associated transcription factors and cytokines in the colon was measured and the T helper type (Th) 17 and regulatory T cell (Treg) subsets were determined in the Peyer's patches. Both L. rhamnosus and B. breve incubations in vitro reduced Th17 and increased Th2 cell subsets in human PBMCs. In addition, B. breve incubation was also able to reduce Th1 and increase Treg cell subsets in contrast to L. rhamnosus. In vivo intervention with B. breve, but not L. rhamnosus, significantly attenuated the severity of DSS-induced colitis. In DSS-treated C57BL/6 mice, intervention with B. breve increased the expression of mRNA encoding for Th2- and Treg-associated cytokines in the distal colon. In addition, intervention with B. breve led to increases of Treg and decreases of Th17 cell subsets in Peyer's patches of DSS-treated mice. B. breve modulates T cell polarization towards Th2 and Treg cell-associated responses in vitro and in vivo. In vivo B. breve intervention ameliorates DSS-induced colitis symptoms and this protective effect may mediated by its effects on the T-cell composition.

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B. breve, but not L. rhamnosus, ameliorates DSS-induced colitis.C57BL/6 mice with or without probiotics treatment received either normal drinking water or drinking water with DSS for 5 days. A) The fecal condition was calculated on day 0, day 3 and day 5 after DSS treatment. On day 6, the mice were sacrificed and B) the colon length of each mouse was measured. Results are expressed as mean ± SEM, n = 6 mice per group, pooled from two independent experiments. Colons were collected and examined for histological score as described in materials and methods. C) The histological scoring graph and D) representative H&E staining photos are shown. Results are expressed as mean + SEM, n = 3 mice per group, pooled from two independent experiments. E) The presence of Ly-6B+ cells was visualized in the proximal (p) and distal (d) colons using immunohistochemistry. The pictures are representative of 3 separate mice per group obtained from two experiments. F) The concentration of MPO was measured in colon homogenates of each group. Results are expressed as mean + SEM, n = 4 mice per group, pooled from two independent experiments. * p<0.05; ** p<0.01.
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pone-0095441-g002: B. breve, but not L. rhamnosus, ameliorates DSS-induced colitis.C57BL/6 mice with or without probiotics treatment received either normal drinking water or drinking water with DSS for 5 days. A) The fecal condition was calculated on day 0, day 3 and day 5 after DSS treatment. On day 6, the mice were sacrificed and B) the colon length of each mouse was measured. Results are expressed as mean ± SEM, n = 6 mice per group, pooled from two independent experiments. Colons were collected and examined for histological score as described in materials and methods. C) The histological scoring graph and D) representative H&E staining photos are shown. Results are expressed as mean + SEM, n = 3 mice per group, pooled from two independent experiments. E) The presence of Ly-6B+ cells was visualized in the proximal (p) and distal (d) colons using immunohistochemistry. The pictures are representative of 3 separate mice per group obtained from two experiments. F) The concentration of MPO was measured in colon homogenates of each group. Results are expressed as mean + SEM, n = 4 mice per group, pooled from two independent experiments. * p<0.05; ** p<0.01.

Mentions: To study the effect of the L. rhamnosus and B. breve strains in vivo, the murine DSS-induced colitis model was used. Mice received L. rhamnosus or B. breve 9 days prior to colitis induction and the bacterial administration was continued until the end of the experiment. Control mice receiving bacteria did not display any clinical changes (data not shown). DSS treatment increased feces condition score, histology score and mildly reduced body weight and the colon length. Intervention with B. breve, but not L. rhamnosus led to improvement of feces condition and to a significant reduction of DSS-induced colon shortening, colon epithelial damage and cellular infiltration as compared to mice with DSS treatment alone (Figure 2A–D).


Bifidobacterium breve attenuates murine dextran sodium sulfate-induced colitis and increases regulatory T cell responses.

Zheng B, van Bergenhenegouwen J, Overbeek S, van de Kant HJ, Garssen J, Folkerts G, Vos P, Morgan ME, Kraneveld AD - PLoS ONE (2014)

B. breve, but not L. rhamnosus, ameliorates DSS-induced colitis.C57BL/6 mice with or without probiotics treatment received either normal drinking water or drinking water with DSS for 5 days. A) The fecal condition was calculated on day 0, day 3 and day 5 after DSS treatment. On day 6, the mice were sacrificed and B) the colon length of each mouse was measured. Results are expressed as mean ± SEM, n = 6 mice per group, pooled from two independent experiments. Colons were collected and examined for histological score as described in materials and methods. C) The histological scoring graph and D) representative H&E staining photos are shown. Results are expressed as mean + SEM, n = 3 mice per group, pooled from two independent experiments. E) The presence of Ly-6B+ cells was visualized in the proximal (p) and distal (d) colons using immunohistochemistry. The pictures are representative of 3 separate mice per group obtained from two experiments. F) The concentration of MPO was measured in colon homogenates of each group. Results are expressed as mean + SEM, n = 4 mice per group, pooled from two independent experiments. * p<0.05; ** p<0.01.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4008378&req=5

pone-0095441-g002: B. breve, but not L. rhamnosus, ameliorates DSS-induced colitis.C57BL/6 mice with or without probiotics treatment received either normal drinking water or drinking water with DSS for 5 days. A) The fecal condition was calculated on day 0, day 3 and day 5 after DSS treatment. On day 6, the mice were sacrificed and B) the colon length of each mouse was measured. Results are expressed as mean ± SEM, n = 6 mice per group, pooled from two independent experiments. Colons were collected and examined for histological score as described in materials and methods. C) The histological scoring graph and D) representative H&E staining photos are shown. Results are expressed as mean + SEM, n = 3 mice per group, pooled from two independent experiments. E) The presence of Ly-6B+ cells was visualized in the proximal (p) and distal (d) colons using immunohistochemistry. The pictures are representative of 3 separate mice per group obtained from two experiments. F) The concentration of MPO was measured in colon homogenates of each group. Results are expressed as mean + SEM, n = 4 mice per group, pooled from two independent experiments. * p<0.05; ** p<0.01.
Mentions: To study the effect of the L. rhamnosus and B. breve strains in vivo, the murine DSS-induced colitis model was used. Mice received L. rhamnosus or B. breve 9 days prior to colitis induction and the bacterial administration was continued until the end of the experiment. Control mice receiving bacteria did not display any clinical changes (data not shown). DSS treatment increased feces condition score, histology score and mildly reduced body weight and the colon length. Intervention with B. breve, but not L. rhamnosus led to improvement of feces condition and to a significant reduction of DSS-induced colon shortening, colon epithelial damage and cellular infiltration as compared to mice with DSS treatment alone (Figure 2A–D).

Bottom Line: In addition, B. breve incubation was also able to reduce Th1 and increase Treg cell subsets in contrast to L. rhamnosus.In addition, intervention with B. breve led to increases of Treg and decreases of Th17 cell subsets in Peyer's patches of DSS-treated mice.B. breve modulates T cell polarization towards Th2 and Treg cell-associated responses in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Utrecht Institute for Pharmaceutical Science, Faculty of Science, Utrecht University, Utrecht, The Netherlands.

ABSTRACT
While some probiotics have shown beneficial effects on preventing or treating colitis development, others have shown no effects. In this study, we have assessed the immunomodulating effects of two probiotic strains, Lactobacillus rhamnosus (L. rhamnosus) and Bifidobacterium breve (B. breve) on T cell polarization in vitro, using human peripheral blood mononuclear cells (PBMC), and in vivo, using murine dextran sodium sulfate (DSS) colitis model. With respect to the latter, the mRNA expression of T cell subset-associated transcription factors and cytokines in the colon was measured and the T helper type (Th) 17 and regulatory T cell (Treg) subsets were determined in the Peyer's patches. Both L. rhamnosus and B. breve incubations in vitro reduced Th17 and increased Th2 cell subsets in human PBMCs. In addition, B. breve incubation was also able to reduce Th1 and increase Treg cell subsets in contrast to L. rhamnosus. In vivo intervention with B. breve, but not L. rhamnosus, significantly attenuated the severity of DSS-induced colitis. In DSS-treated C57BL/6 mice, intervention with B. breve increased the expression of mRNA encoding for Th2- and Treg-associated cytokines in the distal colon. In addition, intervention with B. breve led to increases of Treg and decreases of Th17 cell subsets in Peyer's patches of DSS-treated mice. B. breve modulates T cell polarization towards Th2 and Treg cell-associated responses in vitro and in vivo. In vivo B. breve intervention ameliorates DSS-induced colitis symptoms and this protective effect may mediated by its effects on the T-cell composition.

Show MeSH
Related in: MedlinePlus