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Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients.

He HC, Han ZD, Dai QS, Ling XH, Fu X, Lin ZY, Deng YH, Qin GQ, Cai C, Chen JH, Jiang FN, Liu X, Zhong WD - BMC Genomics (2013)

Bottom Line: The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level.The current studies also identified 15 specific expressed miRNAs in Chinese patients.The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China. xingyinliu@hotmail.com.

ABSTRACT

Background: Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.

Results: A total of 28 miRNAs (fold change ≥ 1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.

Conclusions: Our findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.

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The representative in situ hybridization images of miRNA-374b and miR-19a. The miR-19a in-situ hybridization images in benign tissue (A) and cancer tissue (B); The miR-394b in-situ hybridization images in benign tissue (C) and cancer tissue (D); (E) is the positive control U6 RNA in-situ hybridization images and (F) is the blank control by replacing the RNA probe with PBS solution.
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Figure 5: The representative in situ hybridization images of miRNA-374b and miR-19a. The miR-19a in-situ hybridization images in benign tissue (A) and cancer tissue (B); The miR-394b in-situ hybridization images in benign tissue (C) and cancer tissue (D); (E) is the positive control U6 RNA in-situ hybridization images and (F) is the blank control by replacing the RNA probe with PBS solution.

Mentions: Using in situ hybridization to analyze the expression level of the chosen miRNAs in 104 PCa tissues and 25 adjacent benign prostate tissues, we found that the miR-19a showed up-regulated expression (staining score: PCa = 4.83 ± 0.76 vs. Benign = 4.15 ± 0.55, P = 0.004, Figure 5A–B). Conversely, the expression levels of miRNA-374b and miR-193a-5p in PCa tissues were significantly lower than those in adjacent benign prostate tissues (miRNA-374b staining score: PCa = 3.97 ± 1.17 vs. Benign = 4.70 ± 0.71, P = 0.032, Figure 5C–D; miR-193a-5p staining score: PCa = 3.21 ± 0.60 vs. Benign = 3.60 ± 0.55, P = 0.003). However, miR-188-5p showed no significant expression (data not shown, staining score: PCa = 3.49 ± 1.18 vs. Benign = 3.16 ± 0.78, P = 0.09).Figure 5 shows the representative images of miRNA-374b and miR-19a expression through in situ hybridization.


Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients.

He HC, Han ZD, Dai QS, Ling XH, Fu X, Lin ZY, Deng YH, Qin GQ, Cai C, Chen JH, Jiang FN, Liu X, Zhong WD - BMC Genomics (2013)

The representative in situ hybridization images of miRNA-374b and miR-19a. The miR-19a in-situ hybridization images in benign tissue (A) and cancer tissue (B); The miR-394b in-situ hybridization images in benign tissue (C) and cancer tissue (D); (E) is the positive control U6 RNA in-situ hybridization images and (F) is the blank control by replacing the RNA probe with PBS solution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008360&req=5

Figure 5: The representative in situ hybridization images of miRNA-374b and miR-19a. The miR-19a in-situ hybridization images in benign tissue (A) and cancer tissue (B); The miR-394b in-situ hybridization images in benign tissue (C) and cancer tissue (D); (E) is the positive control U6 RNA in-situ hybridization images and (F) is the blank control by replacing the RNA probe with PBS solution.
Mentions: Using in situ hybridization to analyze the expression level of the chosen miRNAs in 104 PCa tissues and 25 adjacent benign prostate tissues, we found that the miR-19a showed up-regulated expression (staining score: PCa = 4.83 ± 0.76 vs. Benign = 4.15 ± 0.55, P = 0.004, Figure 5A–B). Conversely, the expression levels of miRNA-374b and miR-193a-5p in PCa tissues were significantly lower than those in adjacent benign prostate tissues (miRNA-374b staining score: PCa = 3.97 ± 1.17 vs. Benign = 4.70 ± 0.71, P = 0.032, Figure 5C–D; miR-193a-5p staining score: PCa = 3.21 ± 0.60 vs. Benign = 3.60 ± 0.55, P = 0.003). However, miR-188-5p showed no significant expression (data not shown, staining score: PCa = 3.49 ± 1.18 vs. Benign = 3.16 ± 0.78, P = 0.09).Figure 5 shows the representative images of miRNA-374b and miR-19a expression through in situ hybridization.

Bottom Line: The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level.The current studies also identified 15 specific expressed miRNAs in Chinese patients.The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China. xingyinliu@hotmail.com.

ABSTRACT

Background: Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.

Results: A total of 28 miRNAs (fold change ≥ 1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.

Conclusions: Our findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.

Show MeSH
Related in: MedlinePlus