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Differential serum protein markers and the clinical severity of asthma.

Meyer N, Nuss SJ, Rothe T, Siebenhüner A, Akdis CA, Menz G - J Asthma Allergy (2014)

Bottom Line: During airway inflammation, many cytokines and chemokines are released and some are detectable in the sera.Serum proteins were measured by multiplex analysis.ROC analysis for serum proteins calculated a sensitivity of 55.9% and specificity of 75.8% for discriminating between them.

View Article: PubMed Central - PubMed

Affiliation: Hochgebirgsklinik Davos, Davos-Wolfgang, Switzerland ; Swiss Institute of Allergy and Asthma Research (SIAF), Davos Platz, Switzerland.

ABSTRACT

Background: Asthma is a heterogeneous disease characterized by different clinical phenotypes and the involvement of multiple inflammatory pathways. During airway inflammation, many cytokines and chemokines are released and some are detectable in the sera.

Objective: Serum chemokines and cytokines, involved in airway inflammation in asthma patients, were investigated.

Methods: A total of 191 asthma patients were classified by hierarchical cluster analysis, including the following parameters: forced expiratory volume in 1 second (FEV1), eosinophil cationic protein (ECP) serum levels, blood eosinophils, Junipers asthma symptom score, and the change in FEV1, ECP serum levels, and blood eosinophils after 3 weeks of asthma therapy. Serum proteins were measured by multiplex analysis. Receiver operating characteristic (ROC) curves were used to evaluate the validity of serum proteins for discriminating between asthma clusters.

Results: Classification of asthma patients identified one cluster with high ECP serum levels, increased blood eosinophils, low FEV1 values, and good FEV1 improvement in response to asthma therapy (n=60) and one cluster with low ECP serum levels, low numbers of blood eosinophils, higher FEV1 values, and no FEV1 improvement in response to asthma therapy (n=131). Serum interleukin (IL)-8, eotaxin, vascular endothelial growth factor (VEGF), cutaneous T-cell-attracting chemokine (CTACK), growth-related oncogene (GRO)-α, and hepatocyte growth factor (HGF) were significantly different between the two clusters of asthma patients. ROC analysis for serum proteins calculated a sensitivity of 55.9% and specificity of 75.8% for discriminating between them.

Conclusion: Serum cytokine and chemokine levels might be predictors for the severity of asthmatic inflammation, asthma control, and response to therapy, and therefore might be useful for treatment optimization.

No MeSH data available.


Related in: MedlinePlus

Classification of asthma patients according to airway inflammation.Notes: 191 asthma patients were classified by hierarchical cluster analysis with indicated parameters. (A) Dendrogram. (B) At entry to the high-altitude clinic, FEV1 values, ECP and blood eosinophil levels, and Junipers asthma symptom score were evaluated for cluster 1 and 2. (C) Changes in FEV1, ECP, and blood eosinophils after 3 weeks of asthma therapy from cluster 1 and 2. C1: asthma patients in cluster 1; C2: asthma patients in cluster 2; *P<0.05, ***P<0.001; unpaired t-test was used.Abbreviations: ECP, eosinophil cationic protein; FEV1, forced expiratory volume in 1 second; n, number of asthma patients.
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f1-jaa-7-067: Classification of asthma patients according to airway inflammation.Notes: 191 asthma patients were classified by hierarchical cluster analysis with indicated parameters. (A) Dendrogram. (B) At entry to the high-altitude clinic, FEV1 values, ECP and blood eosinophil levels, and Junipers asthma symptom score were evaluated for cluster 1 and 2. (C) Changes in FEV1, ECP, and blood eosinophils after 3 weeks of asthma therapy from cluster 1 and 2. C1: asthma patients in cluster 1; C2: asthma patients in cluster 2; *P<0.05, ***P<0.001; unpaired t-test was used.Abbreviations: ECP, eosinophil cationic protein; FEV1, forced expiratory volume in 1 second; n, number of asthma patients.

Mentions: Asthma patients were classified according to Junipers symptom score, FEV1, serum ECP, circulating eosinophils, and the improvement in FEV1, ECP, and circulating eosinophils after 3 weeks of therapy in the high-altitude clinic in Davos-Wolfgang. Using hierarchical cluster analysis, two clusters of asthma patients were obtained (Figure 1A). Asthma patients in cluster 1 (n=60) had significantly higher Junipers symptom scores, lower FEV1 values, higher ECP serum levels, and a tendency towards higher blood eosinophils than asthma patients in cluster 2 (n=131) on the day they arrived in the clinic (Figure 1B). The response to asthma therapy differed between these two groups. Asthma patients in cluster 1 showed a higher FEV1 improvement, blood eosinophil decrease, and serum ECP decrease after 3 weeks of intensive asthma therapy (Figure 1C).


Differential serum protein markers and the clinical severity of asthma.

Meyer N, Nuss SJ, Rothe T, Siebenhüner A, Akdis CA, Menz G - J Asthma Allergy (2014)

Classification of asthma patients according to airway inflammation.Notes: 191 asthma patients were classified by hierarchical cluster analysis with indicated parameters. (A) Dendrogram. (B) At entry to the high-altitude clinic, FEV1 values, ECP and blood eosinophil levels, and Junipers asthma symptom score were evaluated for cluster 1 and 2. (C) Changes in FEV1, ECP, and blood eosinophils after 3 weeks of asthma therapy from cluster 1 and 2. C1: asthma patients in cluster 1; C2: asthma patients in cluster 2; *P<0.05, ***P<0.001; unpaired t-test was used.Abbreviations: ECP, eosinophil cationic protein; FEV1, forced expiratory volume in 1 second; n, number of asthma patients.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008293&req=5

f1-jaa-7-067: Classification of asthma patients according to airway inflammation.Notes: 191 asthma patients were classified by hierarchical cluster analysis with indicated parameters. (A) Dendrogram. (B) At entry to the high-altitude clinic, FEV1 values, ECP and blood eosinophil levels, and Junipers asthma symptom score were evaluated for cluster 1 and 2. (C) Changes in FEV1, ECP, and blood eosinophils after 3 weeks of asthma therapy from cluster 1 and 2. C1: asthma patients in cluster 1; C2: asthma patients in cluster 2; *P<0.05, ***P<0.001; unpaired t-test was used.Abbreviations: ECP, eosinophil cationic protein; FEV1, forced expiratory volume in 1 second; n, number of asthma patients.
Mentions: Asthma patients were classified according to Junipers symptom score, FEV1, serum ECP, circulating eosinophils, and the improvement in FEV1, ECP, and circulating eosinophils after 3 weeks of therapy in the high-altitude clinic in Davos-Wolfgang. Using hierarchical cluster analysis, two clusters of asthma patients were obtained (Figure 1A). Asthma patients in cluster 1 (n=60) had significantly higher Junipers symptom scores, lower FEV1 values, higher ECP serum levels, and a tendency towards higher blood eosinophils than asthma patients in cluster 2 (n=131) on the day they arrived in the clinic (Figure 1B). The response to asthma therapy differed between these two groups. Asthma patients in cluster 1 showed a higher FEV1 improvement, blood eosinophil decrease, and serum ECP decrease after 3 weeks of intensive asthma therapy (Figure 1C).

Bottom Line: During airway inflammation, many cytokines and chemokines are released and some are detectable in the sera.Serum proteins were measured by multiplex analysis.ROC analysis for serum proteins calculated a sensitivity of 55.9% and specificity of 75.8% for discriminating between them.

View Article: PubMed Central - PubMed

Affiliation: Hochgebirgsklinik Davos, Davos-Wolfgang, Switzerland ; Swiss Institute of Allergy and Asthma Research (SIAF), Davos Platz, Switzerland.

ABSTRACT

Background: Asthma is a heterogeneous disease characterized by different clinical phenotypes and the involvement of multiple inflammatory pathways. During airway inflammation, many cytokines and chemokines are released and some are detectable in the sera.

Objective: Serum chemokines and cytokines, involved in airway inflammation in asthma patients, were investigated.

Methods: A total of 191 asthma patients were classified by hierarchical cluster analysis, including the following parameters: forced expiratory volume in 1 second (FEV1), eosinophil cationic protein (ECP) serum levels, blood eosinophils, Junipers asthma symptom score, and the change in FEV1, ECP serum levels, and blood eosinophils after 3 weeks of asthma therapy. Serum proteins were measured by multiplex analysis. Receiver operating characteristic (ROC) curves were used to evaluate the validity of serum proteins for discriminating between asthma clusters.

Results: Classification of asthma patients identified one cluster with high ECP serum levels, increased blood eosinophils, low FEV1 values, and good FEV1 improvement in response to asthma therapy (n=60) and one cluster with low ECP serum levels, low numbers of blood eosinophils, higher FEV1 values, and no FEV1 improvement in response to asthma therapy (n=131). Serum interleukin (IL)-8, eotaxin, vascular endothelial growth factor (VEGF), cutaneous T-cell-attracting chemokine (CTACK), growth-related oncogene (GRO)-α, and hepatocyte growth factor (HGF) were significantly different between the two clusters of asthma patients. ROC analysis for serum proteins calculated a sensitivity of 55.9% and specificity of 75.8% for discriminating between them.

Conclusion: Serum cytokine and chemokine levels might be predictors for the severity of asthmatic inflammation, asthma control, and response to therapy, and therefore might be useful for treatment optimization.

No MeSH data available.


Related in: MedlinePlus