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Comparative scanning electron microscopy of bullous diseases.

Almeida HL, Rossi G, Karam OR, Rocha NM, Silva RM - An Bras Dermatol (2014 Mar-Apr)

Bottom Line: The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa).In bullous pemphigoid, the epidermis had a solid aspect.In dystrophic epidermolysis bullosa a net was seen in the blister roof.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Pelotas, Pelotas, RS, Brazil.

ABSTRACT
The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa). In pemphigus foliaceus, acantholytic phenomena were readily demonstrated. In bullous pemphigoid, the epidermis had a solid aspect. In dystrophic epidermolysis bullosa a net was seen in the blister roof.

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SEM of dystrophic epidermolysis bullosa. a) Border of the examined fragment, withartifactitious detachment of the collagen net (large arrow) showing the solidaspect of the cell membrane, with intercellular clefts similar to those shown inFigure 4b (original magnification x3,000). b) adhesion of the collagen net to the cell membrane, showing normalhemidesmosomal function (arrows) (original magnification x5,000)
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f06: SEM of dystrophic epidermolysis bullosa. a) Border of the examined fragment, withartifactitious detachment of the collagen net (large arrow) showing the solidaspect of the cell membrane, with intercellular clefts similar to those shown inFigure 4b (original magnification x3,000). b) adhesion of the collagen net to the cell membrane, showing normalhemidesmosomal function (arrows) (original magnification x5,000)

Mentions: Dystrophic Epidermolysis Bullosa - examination of the inverted blister roofin a DEB case allowed identification of a collagen net attached to the roof,corroborating immunomapping findings, which demonstrated the basal membrane - collagenIV on the blister roof (Figure 5). At highermagnification, an artifactitious detachment of this collagen net was observed in theborder of the examined fragment, showing its interwoven and reticular characteristics(Figure 6A). In the area located behind thedetachment, the basal cell membrane could be observed (similar to Figure 4B). In some areas, under very high magnification, theattachment of the collagen net to the cell membrane could be seen (Figure 6B).


Comparative scanning electron microscopy of bullous diseases.

Almeida HL, Rossi G, Karam OR, Rocha NM, Silva RM - An Bras Dermatol (2014 Mar-Apr)

SEM of dystrophic epidermolysis bullosa. a) Border of the examined fragment, withartifactitious detachment of the collagen net (large arrow) showing the solidaspect of the cell membrane, with intercellular clefts similar to those shown inFigure 4b (original magnification x3,000). b) adhesion of the collagen net to the cell membrane, showing normalhemidesmosomal function (arrows) (original magnification x5,000)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008074&req=5

f06: SEM of dystrophic epidermolysis bullosa. a) Border of the examined fragment, withartifactitious detachment of the collagen net (large arrow) showing the solidaspect of the cell membrane, with intercellular clefts similar to those shown inFigure 4b (original magnification x3,000). b) adhesion of the collagen net to the cell membrane, showing normalhemidesmosomal function (arrows) (original magnification x5,000)
Mentions: Dystrophic Epidermolysis Bullosa - examination of the inverted blister roofin a DEB case allowed identification of a collagen net attached to the roof,corroborating immunomapping findings, which demonstrated the basal membrane - collagenIV on the blister roof (Figure 5). At highermagnification, an artifactitious detachment of this collagen net was observed in theborder of the examined fragment, showing its interwoven and reticular characteristics(Figure 6A). In the area located behind thedetachment, the basal cell membrane could be observed (similar to Figure 4B). In some areas, under very high magnification, theattachment of the collagen net to the cell membrane could be seen (Figure 6B).

Bottom Line: The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa).In bullous pemphigoid, the epidermis had a solid aspect.In dystrophic epidermolysis bullosa a net was seen in the blister roof.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Pelotas, Pelotas, RS, Brazil.

ABSTRACT
The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa). In pemphigus foliaceus, acantholytic phenomena were readily demonstrated. In bullous pemphigoid, the epidermis had a solid aspect. In dystrophic epidermolysis bullosa a net was seen in the blister roof.

Show MeSH
Related in: MedlinePlus