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Comparative scanning electron microscopy of bullous diseases.

Almeida HL, Rossi G, Karam OR, Rocha NM, Silva RM - An Bras Dermatol (2014 Mar-Apr)

Bottom Line: The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa).In bullous pemphigoid, the epidermis had a solid aspect.In dystrophic epidermolysis bullosa a net was seen in the blister roof.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Pelotas, Pelotas, RS, Brazil.

ABSTRACT
The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa). In pemphigus foliaceus, acantholytic phenomena were readily demonstrated. In bullous pemphigoid, the epidermis had a solid aspect. In dystrophic epidermolysis bullosa a net was seen in the blister roof.

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Schematic representation of cleavage levels (arrows) in a normal epidermis stainedwith the APAAP technique. The black line represents the basement membrane (BM).a) Intraepidermal lesion in desmosomal defects. b)Dermoepidermal cleavage in hemidesmosomal defects, with persistence of the BM inthe blister floor. c) Dysfunction of type VII collagen (representedby thin black lines) with cleavage under the BM, which remains attached to theblister roof.
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f01: Schematic representation of cleavage levels (arrows) in a normal epidermis stainedwith the APAAP technique. The black line represents the basement membrane (BM).a) Intraepidermal lesion in desmosomal defects. b)Dermoepidermal cleavage in hemidesmosomal defects, with persistence of the BM inthe blister floor. c) Dysfunction of type VII collagen (representedby thin black lines) with cleavage under the BM, which remains attached to theblister roof.

Mentions: Bullous diseases have different pathogenic factors, and may be due to mutations ofproteins involved in intercellular or dermal-epidermal adhesion or to autoimmune,acquired damage to these proteins.5,6,7The cleavage level depends on the proteins involved and their resulting dysfunctions(Figure 1).


Comparative scanning electron microscopy of bullous diseases.

Almeida HL, Rossi G, Karam OR, Rocha NM, Silva RM - An Bras Dermatol (2014 Mar-Apr)

Schematic representation of cleavage levels (arrows) in a normal epidermis stainedwith the APAAP technique. The black line represents the basement membrane (BM).a) Intraepidermal lesion in desmosomal defects. b)Dermoepidermal cleavage in hemidesmosomal defects, with persistence of the BM inthe blister floor. c) Dysfunction of type VII collagen (representedby thin black lines) with cleavage under the BM, which remains attached to theblister roof.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4008074&req=5

f01: Schematic representation of cleavage levels (arrows) in a normal epidermis stainedwith the APAAP technique. The black line represents the basement membrane (BM).a) Intraepidermal lesion in desmosomal defects. b)Dermoepidermal cleavage in hemidesmosomal defects, with persistence of the BM inthe blister floor. c) Dysfunction of type VII collagen (representedby thin black lines) with cleavage under the BM, which remains attached to theblister roof.
Mentions: Bullous diseases have different pathogenic factors, and may be due to mutations ofproteins involved in intercellular or dermal-epidermal adhesion or to autoimmune,acquired damage to these proteins.5,6,7The cleavage level depends on the proteins involved and their resulting dysfunctions(Figure 1).

Bottom Line: The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa).In bullous pemphigoid, the epidermis had a solid aspect.In dystrophic epidermolysis bullosa a net was seen in the blister roof.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Pelotas, Pelotas, RS, Brazil.

ABSTRACT
The purpose of this study is to compare scanning electron microscopy findings of the blister roof in three distinct bullous diseases: one intraepidermal acantholytic (pemphigus foliaceus); one due to hemidesmosomal dysfunction (bullous pemphigoid); and one secondary to anchoring fibril dysfunction - type VII collagen (dystrophic epidermolysis bullosa). In pemphigus foliaceus, acantholytic phenomena were readily demonstrated. In bullous pemphigoid, the epidermis had a solid aspect. In dystrophic epidermolysis bullosa a net was seen in the blister roof.

Show MeSH
Related in: MedlinePlus