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Endothelial lipase and reverse cholesterol transport in type 2 diabetes mellitus.

Shiu SW, Zhou H, Wong Y, Tan KC - J Diabetes Investig (2010)

Bottom Line: Cholesterol efflux to serum mediated through scavenger receptor class B type I was impaired (15.1 ± 2.5%vs 16.7 ± 3.1, respectively, P < 0.01).Linear regression showed that in controls, HDL, serum EL and waist circumference were major independent determinants of cholesterol efflux; whereas in the diabetic cohort, the major independent determinants of cholesterol efflux were HDL, CRP and age.PWV was increased in the diabetic patients (P < 0.01), but no association between serum EL and PWV was seen in either groups.   Serum EL was increased in diabetic patients, but impaired serum capacity to induce cholesterol efflux in these patients was mainly related to low HDL and subclinical inflammation. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00016.x, 2010).

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, University of Hong Kong, Hong Kong.

ABSTRACT

Unlabelled: Aims/Introduction:  Endothelial lipase (EL) plays an important role in high-density lipoprotein (HDL) metabolism and experimental data suggest that EL might be proatherogenic. We have investigated whether serum EL concentration is associated with changes in serum capacity to induce cholesterol efflux and arterial stiffness in type 2 diabetes.

Materials and methods:   Serum EL was assayed by ELISA in 172 diabetic patients and 175 controls. The ability of serum to induce cholesterol efflux was measured using a cell culture system and arterial stiffness was determined by measuring pulse wave velocity (PWV) between carotid and femoral arteries.

Results:   Diabetic patients had significantly higher C-reactive protein (CRP) and EL (27.7 ± 16.6 ng/mL vs 24.0 ± 11.3, P < 0.05). Cholesterol efflux to serum mediated through scavenger receptor class B type I was impaired (15.1 ± 2.5%vs 16.7 ± 3.1, respectively, P < 0.01). In controls, serum EL correlated with cholesterol efflux to serum (r = -0.16, P = 0.025), but only a trend was seen in the diabetic patients. Linear regression showed that in controls, HDL, serum EL and waist circumference were major independent determinants of cholesterol efflux; whereas in the diabetic cohort, the major independent determinants of cholesterol efflux were HDL, CRP and age. PWV was increased in the diabetic patients (P < 0.01), but no association between serum EL and PWV was seen in either groups.

Conclusions:   Serum EL was increased in diabetic patients, but impaired serum capacity to induce cholesterol efflux in these patients was mainly related to low HDL and subclinical inflammation. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00016.x, 2010).

No MeSH data available.


Related in: MedlinePlus

 Correlation between serum endothelial lipase and scavenger receptor class B type I mediated cholesterol efflux to serum in (a) controls and (b) diabetic patients. SR‐BI, scavenger receptor class B type I.
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f1:  Correlation between serum endothelial lipase and scavenger receptor class B type I mediated cholesterol efflux to serum in (a) controls and (b) diabetic patients. SR‐BI, scavenger receptor class B type I.

Mentions: Univariate analysis was carried out to investigate which clinical parameters were associated with cholesterol efflux. In the control subjects, serum EL correlated inversely with SR‐BI mediated cholesterol efflux (Figure 1a) but not with ABCA1 mediated cholesterol efflux. SR‐BI mediated cholesterol efflux to serum also correlated with HDL (r = 0.59, P < 0.001), APOAI (r = 0.60, P < 0.001), waist circumference (r = −0.16, P < 0.05), but not with logCRP. However, in the diabetic patients, there was only a trend towards an association between serum EL and SR‐BI mediated cholesterol efflux (Figure 1b) and there was no correlation with ABCA1 mediated cholesterol efflux. In the diabetic cohort, SR‐BI mediated cholesterol efflux to serum correlated with HDL (r = 0.62, P < 0.001), APOAI (r = 0.51, P < 0.001), waist circumference (r = −0.19, P < 0.05) and logCRP (r = −0.32, P < 0.001), whereas ABCA1 mediated cholesterol efflux to serum correlated only with HDL (r = 0.23, P < 0.01).


Endothelial lipase and reverse cholesterol transport in type 2 diabetes mellitus.

Shiu SW, Zhou H, Wong Y, Tan KC - J Diabetes Investig (2010)

 Correlation between serum endothelial lipase and scavenger receptor class B type I mediated cholesterol efflux to serum in (a) controls and (b) diabetic patients. SR‐BI, scavenger receptor class B type I.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4008025&req=5

f1:  Correlation between serum endothelial lipase and scavenger receptor class B type I mediated cholesterol efflux to serum in (a) controls and (b) diabetic patients. SR‐BI, scavenger receptor class B type I.
Mentions: Univariate analysis was carried out to investigate which clinical parameters were associated with cholesterol efflux. In the control subjects, serum EL correlated inversely with SR‐BI mediated cholesterol efflux (Figure 1a) but not with ABCA1 mediated cholesterol efflux. SR‐BI mediated cholesterol efflux to serum also correlated with HDL (r = 0.59, P < 0.001), APOAI (r = 0.60, P < 0.001), waist circumference (r = −0.16, P < 0.05), but not with logCRP. However, in the diabetic patients, there was only a trend towards an association between serum EL and SR‐BI mediated cholesterol efflux (Figure 1b) and there was no correlation with ABCA1 mediated cholesterol efflux. In the diabetic cohort, SR‐BI mediated cholesterol efflux to serum correlated with HDL (r = 0.62, P < 0.001), APOAI (r = 0.51, P < 0.001), waist circumference (r = −0.19, P < 0.05) and logCRP (r = −0.32, P < 0.001), whereas ABCA1 mediated cholesterol efflux to serum correlated only with HDL (r = 0.23, P < 0.01).

Bottom Line: Cholesterol efflux to serum mediated through scavenger receptor class B type I was impaired (15.1 ± 2.5%vs 16.7 ± 3.1, respectively, P < 0.01).Linear regression showed that in controls, HDL, serum EL and waist circumference were major independent determinants of cholesterol efflux; whereas in the diabetic cohort, the major independent determinants of cholesterol efflux were HDL, CRP and age.PWV was increased in the diabetic patients (P < 0.01), but no association between serum EL and PWV was seen in either groups.   Serum EL was increased in diabetic patients, but impaired serum capacity to induce cholesterol efflux in these patients was mainly related to low HDL and subclinical inflammation. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00016.x, 2010).

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, University of Hong Kong, Hong Kong.

ABSTRACT

Unlabelled: Aims/Introduction:  Endothelial lipase (EL) plays an important role in high-density lipoprotein (HDL) metabolism and experimental data suggest that EL might be proatherogenic. We have investigated whether serum EL concentration is associated with changes in serum capacity to induce cholesterol efflux and arterial stiffness in type 2 diabetes.

Materials and methods:   Serum EL was assayed by ELISA in 172 diabetic patients and 175 controls. The ability of serum to induce cholesterol efflux was measured using a cell culture system and arterial stiffness was determined by measuring pulse wave velocity (PWV) between carotid and femoral arteries.

Results:   Diabetic patients had significantly higher C-reactive protein (CRP) and EL (27.7 ± 16.6 ng/mL vs 24.0 ± 11.3, P < 0.05). Cholesterol efflux to serum mediated through scavenger receptor class B type I was impaired (15.1 ± 2.5%vs 16.7 ± 3.1, respectively, P < 0.01). In controls, serum EL correlated with cholesterol efflux to serum (r = -0.16, P = 0.025), but only a trend was seen in the diabetic patients. Linear regression showed that in controls, HDL, serum EL and waist circumference were major independent determinants of cholesterol efflux; whereas in the diabetic cohort, the major independent determinants of cholesterol efflux were HDL, CRP and age. PWV was increased in the diabetic patients (P < 0.01), but no association between serum EL and PWV was seen in either groups.

Conclusions:   Serum EL was increased in diabetic patients, but impaired serum capacity to induce cholesterol efflux in these patients was mainly related to low HDL and subclinical inflammation. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00016.x, 2010).

No MeSH data available.


Related in: MedlinePlus