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Safety and efficacy of suicide gene therapy with adenosine deaminase 5-fluorocytosine silmutaneously in in vitro cultures of melanoma and retinal cell lines.

Sakkas A, Zarogoulidis P, Domvri K, Hohenforst-Schmidt W, Bougiouklis D, Kakolyris S, Zarampoukas T, Kioumis I, Pitsiou G, Huang H, Li Q, Meditskou S, Tsiouda T, Pezirkianidis N, Zarogoulidis K - J Cancer (2014)

Bottom Line: Adenosine Cytosine Deaminase (Ad.CD) was also used in order to convert the pro-drug 5-Flucytosine to the active 5-Fluoracil.At indicated time-points (4h, 8h and 24h) cell viability and apoptosis were measured.Indeed, our results indicated that in every 5-FC administration had mild cytotoxicity for the retinal cells, while increased apoptosis was observed for the melanoma cell line.

View Article: PubMed Central - PubMed

Affiliation: 1. Pulmonary Department-Oncology Unit, ``G. Papanikolaou`` General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.

ABSTRACT
Local treatment as a treatment modality is gaining increased general acceptance over time. Novel drugs and methodologies of local administration are being investigated in an effort to achieve disease local control. Suicide gene therapy is a method that has been investigated as a local treatment with simultaneously distant disease control. In our current experiment we purchased HTB-70 (melanoma cell line, derived from metastatic axillary node) and CRL-2302 (human retinal epithelium) were from ATCC LGC Standards and Ancotil(®), 2.5 g/250 ml (1 g/00ml) (5-Flucytosine) MEDA; Pharmaceuticals Ltd. UK. Adenosine Cytosine Deaminase (Ad.CD) was also used in order to convert the pro-drug 5-Flucytosine to the active 5-Fluoracil. Three different concentrations of 5-Flucytosine (5-FC) were administered (0.2ml, 0.8ml and 1.2ml). At indicated time-points (4h, 8h and 24h) cell viability and apoptosis were measured. Our concept was to investigate whether suicide gene therapy with Ad. CD-5-FC could be used with safety and efficiency as a future local treatment for melanoma located in the eye cavity. Indeed, our results indicated that in every 5-FC administration had mild cytotoxicity for the retinal cells, while increased apoptosis was observed for the melanoma cell line.

No MeSH data available.


Related in: MedlinePlus

A) Melanoma cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, B) Retinal cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, C) Melanoma cell 0.8mg ancotil and viability at 4 hours with annexin, D) Retinal cells 0.8 mg ancotil and viability at 4 hours with annexin.
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Figure 3: A) Melanoma cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, B) Retinal cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, C) Melanoma cell 0.8mg ancotil and viability at 4 hours with annexin, D) Retinal cells 0.8 mg ancotil and viability at 4 hours with annexin.


Safety and efficacy of suicide gene therapy with adenosine deaminase 5-fluorocytosine silmutaneously in in vitro cultures of melanoma and retinal cell lines.

Sakkas A, Zarogoulidis P, Domvri K, Hohenforst-Schmidt W, Bougiouklis D, Kakolyris S, Zarampoukas T, Kioumis I, Pitsiou G, Huang H, Li Q, Meditskou S, Tsiouda T, Pezirkianidis N, Zarogoulidis K - J Cancer (2014)

A) Melanoma cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, B) Retinal cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, C) Melanoma cell 0.8mg ancotil and viability at 4 hours with annexin, D) Retinal cells 0.8 mg ancotil and viability at 4 hours with annexin.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4007525&req=5

Figure 3: A) Melanoma cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, B) Retinal cells 0.8 mg ancotil and viability at 4 hours with 7-AAD, C) Melanoma cell 0.8mg ancotil and viability at 4 hours with annexin, D) Retinal cells 0.8 mg ancotil and viability at 4 hours with annexin.
Bottom Line: Adenosine Cytosine Deaminase (Ad.CD) was also used in order to convert the pro-drug 5-Flucytosine to the active 5-Fluoracil.At indicated time-points (4h, 8h and 24h) cell viability and apoptosis were measured.Indeed, our results indicated that in every 5-FC administration had mild cytotoxicity for the retinal cells, while increased apoptosis was observed for the melanoma cell line.

View Article: PubMed Central - PubMed

Affiliation: 1. Pulmonary Department-Oncology Unit, ``G. Papanikolaou`` General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.

ABSTRACT
Local treatment as a treatment modality is gaining increased general acceptance over time. Novel drugs and methodologies of local administration are being investigated in an effort to achieve disease local control. Suicide gene therapy is a method that has been investigated as a local treatment with simultaneously distant disease control. In our current experiment we purchased HTB-70 (melanoma cell line, derived from metastatic axillary node) and CRL-2302 (human retinal epithelium) were from ATCC LGC Standards and Ancotil(®), 2.5 g/250 ml (1 g/00ml) (5-Flucytosine) MEDA; Pharmaceuticals Ltd. UK. Adenosine Cytosine Deaminase (Ad.CD) was also used in order to convert the pro-drug 5-Flucytosine to the active 5-Fluoracil. Three different concentrations of 5-Flucytosine (5-FC) were administered (0.2ml, 0.8ml and 1.2ml). At indicated time-points (4h, 8h and 24h) cell viability and apoptosis were measured. Our concept was to investigate whether suicide gene therapy with Ad. CD-5-FC could be used with safety and efficiency as a future local treatment for melanoma located in the eye cavity. Indeed, our results indicated that in every 5-FC administration had mild cytotoxicity for the retinal cells, while increased apoptosis was observed for the melanoma cell line.

No MeSH data available.


Related in: MedlinePlus