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Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.

Kersten B, Ghirardo A, Schnitzler JP, Kanawati B, Schmitt-Kopplin P, Fladung M, Schroeder H - BMC Genomics (2013)

Bottom Line: Next generation RNA sequencing revealed hundreds of genes that exhibited constitutive and/or inducible differential expression in the resistant oak compared to the susceptible oak.We conclude that the resistant oak type seem to prefer a strategy of constitutive defence responses in contrast to more induced defence responses of the susceptible oaks triggered by feeding.These results pave the way for the development of biomarkers for an early determination of potentially green oak leaf roller-resistant genotypes in natural pedunculate oak populations in Europe.

View Article: PubMed Central - HTML - PubMed

Affiliation: Thünen Institute of Forest Genetics, Sieker Landstrasse 2, D-22927, Grosshansdorf, Germany. hilke.schroeder@ti.bund.de.

ABSTRACT

Background: The interaction between insect pests and their host plants is a never-ending race of evolutionary adaption. Plants have developed an armament against insect herbivore attacks, and attackers continuously learn how to address it. Using a combined transcriptomic and metabolomic approach, we investigated the molecular and biochemical differences between Quercus robur L. trees that resisted (defined as resistant oak type) or were susceptible (defined as susceptible oak type) to infestation by the major oak pest, Tortrix viridana L.

Results: Next generation RNA sequencing revealed hundreds of genes that exhibited constitutive and/or inducible differential expression in the resistant oak compared to the susceptible oak. Distinct differences were found in the transcript levels and the metabolic content with regard to tannins, flavonoids, and terpenoids, which are compounds involved in the defence against insect pests. The results of our transcriptomic and metabolomic analyses are in agreement with those of a previous study in which we showed that female moths prefer susceptible oaks due to their specific profile of herbivore-induced volatiles. These data therefore define two oak genotypes that clearly differ on the transcriptomic and metabolomic levels, as reflected by their specific defensive compound profiles.

Conclusions: We conclude that the resistant oak type seem to prefer a strategy of constitutive defence responses in contrast to more induced defence responses of the susceptible oaks triggered by feeding. These results pave the way for the development of biomarkers for an early determination of potentially green oak leaf roller-resistant genotypes in natural pedunculate oak populations in Europe.

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Principle component analysis (PCA) of non-target metabolomics. Metabolic differences between T- (red) and S-oaks (blue) from damaged (Td and Sd) and intact (Ti and Si) leaves. All plants were fed by T.viridana for 32 h and the leaves were 'old’ (6–8 weeks after budbreak) (PCA1 = 8% and PCA2 = 7% of total X-variable variation).
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Figure 6: Principle component analysis (PCA) of non-target metabolomics. Metabolic differences between T- (red) and S-oaks (blue) from damaged (Td and Sd) and intact (Ti and Si) leaves. All plants were fed by T.viridana for 32 h and the leaves were 'old’ (6–8 weeks after budbreak) (PCA1 = 8% and PCA2 = 7% of total X-variable variation).

Mentions: Principal components analysis (PCA) identified clear metabolic differences between T- and S-oaks at 32 h after the onset of feeding by T. viridana larvae (Figure 6) by explaining a total of 15% of the variance in metabolites differences. Furthermore, the distinct metabolic profiles of intact (I) and directly damaged (D) leaves showed that local and/or systemic defence responses were induced in the plant within 32 h of herbivore feeding.


Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.

Kersten B, Ghirardo A, Schnitzler JP, Kanawati B, Schmitt-Kopplin P, Fladung M, Schroeder H - BMC Genomics (2013)

Principle component analysis (PCA) of non-target metabolomics. Metabolic differences between T- (red) and S-oaks (blue) from damaged (Td and Sd) and intact (Ti and Si) leaves. All plants were fed by T.viridana for 32 h and the leaves were 'old’ (6–8 weeks after budbreak) (PCA1 = 8% and PCA2 = 7% of total X-variable variation).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4007517&req=5

Figure 6: Principle component analysis (PCA) of non-target metabolomics. Metabolic differences between T- (red) and S-oaks (blue) from damaged (Td and Sd) and intact (Ti and Si) leaves. All plants were fed by T.viridana for 32 h and the leaves were 'old’ (6–8 weeks after budbreak) (PCA1 = 8% and PCA2 = 7% of total X-variable variation).
Mentions: Principal components analysis (PCA) identified clear metabolic differences between T- and S-oaks at 32 h after the onset of feeding by T. viridana larvae (Figure 6) by explaining a total of 15% of the variance in metabolites differences. Furthermore, the distinct metabolic profiles of intact (I) and directly damaged (D) leaves showed that local and/or systemic defence responses were induced in the plant within 32 h of herbivore feeding.

Bottom Line: Next generation RNA sequencing revealed hundreds of genes that exhibited constitutive and/or inducible differential expression in the resistant oak compared to the susceptible oak.We conclude that the resistant oak type seem to prefer a strategy of constitutive defence responses in contrast to more induced defence responses of the susceptible oaks triggered by feeding.These results pave the way for the development of biomarkers for an early determination of potentially green oak leaf roller-resistant genotypes in natural pedunculate oak populations in Europe.

View Article: PubMed Central - HTML - PubMed

Affiliation: Thünen Institute of Forest Genetics, Sieker Landstrasse 2, D-22927, Grosshansdorf, Germany. hilke.schroeder@ti.bund.de.

ABSTRACT

Background: The interaction between insect pests and their host plants is a never-ending race of evolutionary adaption. Plants have developed an armament against insect herbivore attacks, and attackers continuously learn how to address it. Using a combined transcriptomic and metabolomic approach, we investigated the molecular and biochemical differences between Quercus robur L. trees that resisted (defined as resistant oak type) or were susceptible (defined as susceptible oak type) to infestation by the major oak pest, Tortrix viridana L.

Results: Next generation RNA sequencing revealed hundreds of genes that exhibited constitutive and/or inducible differential expression in the resistant oak compared to the susceptible oak. Distinct differences were found in the transcript levels and the metabolic content with regard to tannins, flavonoids, and terpenoids, which are compounds involved in the defence against insect pests. The results of our transcriptomic and metabolomic analyses are in agreement with those of a previous study in which we showed that female moths prefer susceptible oaks due to their specific profile of herbivore-induced volatiles. These data therefore define two oak genotypes that clearly differ on the transcriptomic and metabolomic levels, as reflected by their specific defensive compound profiles.

Conclusions: We conclude that the resistant oak type seem to prefer a strategy of constitutive defence responses in contrast to more induced defence responses of the susceptible oaks triggered by feeding. These results pave the way for the development of biomarkers for an early determination of potentially green oak leaf roller-resistant genotypes in natural pedunculate oak populations in Europe.

Show MeSH
Related in: MedlinePlus