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Increase in sphingolipid catabolic enzyme activity during aging.

Sacket SJ, Chung HY, Okajima F, Im DS - Acta Pharmacol. Sin. (2009)

Bottom Line: The activities of sphingolipid metabolic enzymes were significantly elevated in all tested tissues during development and aging, although the onset of significant increase in activity varied on the tissue and enzyme type.During aging, 18 out of 21 enzyme activities were further increased on day 720 compared to day 180.Differential increases in sphingolipid metabolic enzyme activities suggest that sphingolipids including ceramide and sphingosine might play important and dynamic roles in proliferation, differentiation and apoptosis during development and aging.

View Article: PubMed Central - PubMed

Affiliation: Laboratories of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Institute of Life Science and Technology, Pusan National University, Busan 609-735, Republic of Korea.

ABSTRACT

Aim: To understand the contribution of sphingolipid metabolism and its metabolites to development and aging.

Methods: A systemic analysis on the changes in activity of sphingolipid metabolic enzymes in kidney, liver and brain tissues during development and aging was conducted. The study was conducted using tissues from 1-day-old to 720-day-old rats.

Results: Catabolic enzyme activities as well as the level of sphingomyelinase (SMase) and ceramidase (CDase) were higher than that of anabolic enzyme activities, sphingomyelin synthase and ceramide synthase. This suggested an accumulation of ceramide and sphingosine during development and aging. The liver showed the highest neutral-SMase activity among the tested enzymes while the kidney and brain exhibited higher neutral-SMase and ceramidase activities, indicating a high production of ceramide in liver and ceramide/sphingosine in the kidney and brain. The activities of sphingolipid metabolic enzymes were significantly elevated in all tested tissues during development and aging, although the onset of significant increase in activity varied on the tissue and enzyme type. During aging, 18 out of 21 enzyme activities were further increased on day 720 compared to day 180.

Conclusion: Differential increases in sphingolipid metabolic enzyme activities suggest that sphingolipids including ceramide and sphingosine might play important and dynamic roles in proliferation, differentiation and apoptosis during development and aging.

Show MeSH
Ceramide synthase activity in rat kidney, liver and brain tissues. Ceramide synthase activities were measured from 1-day, 7-day, 21-day, 42-day, 180-day, 510-day, and 720-day-old rat kidney, liver and brain tissues. Rat tissue homogenates were incubated with [3H]-sphingosine. Lipids were extracted and [3H]-ceramide isolated on TLC by autoradiography and quantitated using a liquid scintillation counter.
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fig4: Ceramide synthase activity in rat kidney, liver and brain tissues. Ceramide synthase activities were measured from 1-day, 7-day, 21-day, 42-day, 180-day, 510-day, and 720-day-old rat kidney, liver and brain tissues. Rat tissue homogenates were incubated with [3H]-sphingosine. Lipids were extracted and [3H]-ceramide isolated on TLC by autoradiography and quantitated using a liquid scintillation counter.

Mentions: Next, we measured the ceramide synthase activity in the same set of tissues. We found that the enzyme activity was increased with development from day 21 in the rat kidney, liver and brain (Figure 4). We also observed that ceramide synthase activity in those tissues was drastically lower compared to other sphingolipid catabolic enzyme activities like SMase and CDase; however, a further increase in ceramide synthase was observed in three tissues from day 180 (Figures 4 and 5). Thus, the rate of ceramide generation from sphingosine might be low and comparable to the rate of sphingomyelin generation from ceramide.


Increase in sphingolipid catabolic enzyme activity during aging.

Sacket SJ, Chung HY, Okajima F, Im DS - Acta Pharmacol. Sin. (2009)

Ceramide synthase activity in rat kidney, liver and brain tissues. Ceramide synthase activities were measured from 1-day, 7-day, 21-day, 42-day, 180-day, 510-day, and 720-day-old rat kidney, liver and brain tissues. Rat tissue homogenates were incubated with [3H]-sphingosine. Lipids were extracted and [3H]-ceramide isolated on TLC by autoradiography and quantitated using a liquid scintillation counter.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4007327&req=5

fig4: Ceramide synthase activity in rat kidney, liver and brain tissues. Ceramide synthase activities were measured from 1-day, 7-day, 21-day, 42-day, 180-day, 510-day, and 720-day-old rat kidney, liver and brain tissues. Rat tissue homogenates were incubated with [3H]-sphingosine. Lipids were extracted and [3H]-ceramide isolated on TLC by autoradiography and quantitated using a liquid scintillation counter.
Mentions: Next, we measured the ceramide synthase activity in the same set of tissues. We found that the enzyme activity was increased with development from day 21 in the rat kidney, liver and brain (Figure 4). We also observed that ceramide synthase activity in those tissues was drastically lower compared to other sphingolipid catabolic enzyme activities like SMase and CDase; however, a further increase in ceramide synthase was observed in three tissues from day 180 (Figures 4 and 5). Thus, the rate of ceramide generation from sphingosine might be low and comparable to the rate of sphingomyelin generation from ceramide.

Bottom Line: The activities of sphingolipid metabolic enzymes were significantly elevated in all tested tissues during development and aging, although the onset of significant increase in activity varied on the tissue and enzyme type.During aging, 18 out of 21 enzyme activities were further increased on day 720 compared to day 180.Differential increases in sphingolipid metabolic enzyme activities suggest that sphingolipids including ceramide and sphingosine might play important and dynamic roles in proliferation, differentiation and apoptosis during development and aging.

View Article: PubMed Central - PubMed

Affiliation: Laboratories of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Institute of Life Science and Technology, Pusan National University, Busan 609-735, Republic of Korea.

ABSTRACT

Aim: To understand the contribution of sphingolipid metabolism and its metabolites to development and aging.

Methods: A systemic analysis on the changes in activity of sphingolipid metabolic enzymes in kidney, liver and brain tissues during development and aging was conducted. The study was conducted using tissues from 1-day-old to 720-day-old rats.

Results: Catabolic enzyme activities as well as the level of sphingomyelinase (SMase) and ceramidase (CDase) were higher than that of anabolic enzyme activities, sphingomyelin synthase and ceramide synthase. This suggested an accumulation of ceramide and sphingosine during development and aging. The liver showed the highest neutral-SMase activity among the tested enzymes while the kidney and brain exhibited higher neutral-SMase and ceramidase activities, indicating a high production of ceramide in liver and ceramide/sphingosine in the kidney and brain. The activities of sphingolipid metabolic enzymes were significantly elevated in all tested tissues during development and aging, although the onset of significant increase in activity varied on the tissue and enzyme type. During aging, 18 out of 21 enzyme activities were further increased on day 720 compared to day 180.

Conclusion: Differential increases in sphingolipid metabolic enzyme activities suggest that sphingolipids including ceramide and sphingosine might play important and dynamic roles in proliferation, differentiation and apoptosis during development and aging.

Show MeSH