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The impact of extracellular and intracellular Ca2+ on ethanol-induced smooth muscle contraction.

Döndaş NY, Kaplan M, Kaya D, Singirik E - Acta Pharmacol. Sin. (2009)

Bottom Line: Although lidocaine (50 and 100 micromol/L), a local anesthetic agent, and hexamethonium (100 and 500 micromol/L), a ganglionic blocking agent, failed to affect these contractions, verapamil (1-50 micromol/L) and nifedipine (1-50 micromol/L), selective blockers of L-type Ca2+ channels, significantly inhibited the contractile responses of ethanol.Ryanodine (1-50 micromol/L) and ruthenium red (10-100 micromol/L), selective blockers of intracellular Ca2+ channels/ryanodine receptors; cyclopiazonic acid (CPA; 1-10 mumol/L), a selective inhibitor of sarcoplasmic reticulum (SR) Ca(2+)-ATPase; and caffeine (0.5-5 mmol/L), a depleting agent of intracellular Ca2+ stores, significantly inhibited the contractile responses induced by ethanol.This inhibition was significantly different from those associated with caffeine, ryanodine or CPA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Cukurova University, 01330 Adana, Turkey. yakdas25@cu.edu.tr

ABSTRACT

Aim: To evaluate the impact of extracellular and intracellular Ca2+ on contractions induced by ethanol in smooth muscle.

Methods: Longitudinal smooth muscle strips were prepared from the gastric fundi of mice. The contractions of smooth muscle strips were recorded with an isometric force displacement transducer.

Results: Ethanol (164 mmol/L) produced reproducible contractions in isolated gastric fundal strips of mice. Although lidocaine (50 and 100 micromol/L), a local anesthetic agent, and hexamethonium (100 and 500 micromol/L), a ganglionic blocking agent, failed to affect these contractions, verapamil (1-50 micromol/L) and nifedipine (1-50 micromol/L), selective blockers of L-type Ca2+ channels, significantly inhibited the contractile responses of ethanol. Using a Ca(2+)-free medium nearly eliminated these contractions in the same tissue. Ryanodine (1-50 micromol/L) and ruthenium red (10-100 micromol/L), selective blockers of intracellular Ca2+ channels/ryanodine receptors; cyclopiazonic acid (CPA; 1-10 mumol/L), a selective inhibitor of sarcoplasmic reticulum (SR) Ca(2+)-ATPase; and caffeine (0.5-5 mmol/L), a depleting agent of intracellular Ca2+ stores, significantly inhibited the contractile responses induced by ethanol. In addition, the combination of caffeine (5 mmol/L) plus CPA (10 micromol/L), and ryanodine (10 micromol/L) plus CPA (10 micromol/L), caused further inhibition of contractions in response to ethanol. This inhibition was significantly different from those associated with caffeine, ryanodine or CPA. Furthermore the combination of caffeine (5 mmol/L), ryanodine (10 micromol/L) and CPA(10 micromol/L) eliminated the contractions induced by ethanol in isolated gastric fundal strips of mice.

Conclusion: Both extracellular and intracellular Ca2+ may have important roles in regulating contractions induced by ethanol in the mouse gastric fundus.

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A typical tracing showing reproducible contractile responses induced by ethanol (164 mmol/L) in isolated gastric fundal strips of mice (general control group). EtOH: ethanol, W: wash, mN: milli Newton.
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fig1: A typical tracing showing reproducible contractile responses induced by ethanol (164 mmol/L) in isolated gastric fundal strips of mice (general control group). EtOH: ethanol, W: wash, mN: milli Newton.

Mentions: Ethanol produced reproducible contractions in the isolated gastric fundal strips of mice. In each experiment, two contractile responses were analyzed (Table 1, Figure 1). In all experiments, after the experimental protocol was completed, the preparation was washed and the third contractile response was obtained.


The impact of extracellular and intracellular Ca2+ on ethanol-induced smooth muscle contraction.

Döndaş NY, Kaplan M, Kaya D, Singirik E - Acta Pharmacol. Sin. (2009)

A typical tracing showing reproducible contractile responses induced by ethanol (164 mmol/L) in isolated gastric fundal strips of mice (general control group). EtOH: ethanol, W: wash, mN: milli Newton.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4007325&req=5

fig1: A typical tracing showing reproducible contractile responses induced by ethanol (164 mmol/L) in isolated gastric fundal strips of mice (general control group). EtOH: ethanol, W: wash, mN: milli Newton.
Mentions: Ethanol produced reproducible contractions in the isolated gastric fundal strips of mice. In each experiment, two contractile responses were analyzed (Table 1, Figure 1). In all experiments, after the experimental protocol was completed, the preparation was washed and the third contractile response was obtained.

Bottom Line: Although lidocaine (50 and 100 micromol/L), a local anesthetic agent, and hexamethonium (100 and 500 micromol/L), a ganglionic blocking agent, failed to affect these contractions, verapamil (1-50 micromol/L) and nifedipine (1-50 micromol/L), selective blockers of L-type Ca2+ channels, significantly inhibited the contractile responses of ethanol.Ryanodine (1-50 micromol/L) and ruthenium red (10-100 micromol/L), selective blockers of intracellular Ca2+ channels/ryanodine receptors; cyclopiazonic acid (CPA; 1-10 mumol/L), a selective inhibitor of sarcoplasmic reticulum (SR) Ca(2+)-ATPase; and caffeine (0.5-5 mmol/L), a depleting agent of intracellular Ca2+ stores, significantly inhibited the contractile responses induced by ethanol.This inhibition was significantly different from those associated with caffeine, ryanodine or CPA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Cukurova University, 01330 Adana, Turkey. yakdas25@cu.edu.tr

ABSTRACT

Aim: To evaluate the impact of extracellular and intracellular Ca2+ on contractions induced by ethanol in smooth muscle.

Methods: Longitudinal smooth muscle strips were prepared from the gastric fundi of mice. The contractions of smooth muscle strips were recorded with an isometric force displacement transducer.

Results: Ethanol (164 mmol/L) produced reproducible contractions in isolated gastric fundal strips of mice. Although lidocaine (50 and 100 micromol/L), a local anesthetic agent, and hexamethonium (100 and 500 micromol/L), a ganglionic blocking agent, failed to affect these contractions, verapamil (1-50 micromol/L) and nifedipine (1-50 micromol/L), selective blockers of L-type Ca2+ channels, significantly inhibited the contractile responses of ethanol. Using a Ca(2+)-free medium nearly eliminated these contractions in the same tissue. Ryanodine (1-50 micromol/L) and ruthenium red (10-100 micromol/L), selective blockers of intracellular Ca2+ channels/ryanodine receptors; cyclopiazonic acid (CPA; 1-10 mumol/L), a selective inhibitor of sarcoplasmic reticulum (SR) Ca(2+)-ATPase; and caffeine (0.5-5 mmol/L), a depleting agent of intracellular Ca2+ stores, significantly inhibited the contractile responses induced by ethanol. In addition, the combination of caffeine (5 mmol/L) plus CPA (10 micromol/L), and ryanodine (10 micromol/L) plus CPA (10 micromol/L), caused further inhibition of contractions in response to ethanol. This inhibition was significantly different from those associated with caffeine, ryanodine or CPA. Furthermore the combination of caffeine (5 mmol/L), ryanodine (10 micromol/L) and CPA(10 micromol/L) eliminated the contractions induced by ethanol in isolated gastric fundal strips of mice.

Conclusion: Both extracellular and intracellular Ca2+ may have important roles in regulating contractions induced by ethanol in the mouse gastric fundus.

Show MeSH
Related in: MedlinePlus